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Citations for GE Life Sciences :
151 - 200 of 4068 citations for Prestained Protein Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC) using an AKTA purifier system (GE Healthcare), eluting with a linear gradient of 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated with standard erythrocyte lysis buffer on ice and separated from myelin and debris by Percoll gradient (GE Healthcare) centrifugation ...
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bioRxiv - Biochemistry 2019Quote: ... and purified using a standard step gradient (300 mM imidazol) immobilized metal affinity chromatography (IMAC) protocol (Ni-Sepharose Fast Flow, GE Healthcare). Afterwards ...
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bioRxiv - Biochemistry 2020Quote: ... cells applied to a thin SD-Ura agar pad on a standard microscopy slide and imaged live on a DeltaVision Elite Widefield Deconvolution Microscope (GE Healthcare) using a 100 x oil immersion objective with a sCMOS camera (Teledyne Photometics) ...
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bioRxiv - Biochemistry 2021Quote: ... Western blotting was performed with standard protocols using the PVDF membrane and signals visualized with ImageQuant LAS 4000 Luminescent Image Analyzer (GE Healthcare).
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bioRxiv - Synthetic Biology 2021Quote: ... 66 kDa) were used to generate a molecular weight standard plot using a Superdex 200 10/300 GL column (GE Healthcare). A 3.2 mg/ml sample of His-PodJ(1-635 ...
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bioRxiv - Bioengineering 2022Quote: Ultrasound images of the incubated arteries were obtained with a standard clinical vascular ultrasound machine with a 10 MHz linear array transducer (LOGIQ V2, GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2021Quote: Western blotting was carried out using standard methods with semi-dry blotting and the nitrocellulose blots were developed using ECL (GE Healthcare). The images were recorded with a BioRad ChemiDoc MP imager ...
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bioRxiv - Microbiology 2020Quote: ... The molecular weight of AmvR in solution was estimated by comparing its elution volume to those of a set of molecular weight standards (HMW and LMW calibration sets, GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Equal amounts of lysates containing Laemmli buffer were heated at 95°C and electrophoresed on 12% polyacrylamide gels under standard SDS-PAGE conditions before being transferred onto a polyvinylidene fluoride membranes (GE Healthcare). Protein loaded membranes were blocked with 5% (w/v ...
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bioRxiv - Immunology 2022Quote: ... Umbilical cord blood mononuclear cells (UCBMC) and plasma were obtained by standard density gradient centrifugation over Ficoll (GE Healthcare, Chicago, IL). UCBMC were frozen in 10% DMSO/FetalPlex (GeminiBio ...
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bioRxiv - Immunology 2022Quote: ... Samples were resolved by SDS-12% polyacrylamide gel electrophoresis under standard conditions and transferred to a polyvinylidene difluoride (PVDF) membrane (Amersham Hybond, GE Healthcare) using the Trans-Blot turbo transfer system (Biorad) ...
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bioRxiv - Microbiology 2022Quote: ... and the supernatant was injected into a Superdex 200 increase 10/300 GL (cytiva) column calibrated with known molecular weight standards (GE healthcare). Size exclusion chromatography was then performed at a flow rate of 0.5 ml/min in a buffer containing 20 mM HEPES pH 7.2 ...
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bioRxiv - Neuroscience 2023Quote: ... and pellets were resuspended in 6 mL 25 % Standard Isotonic Percoll (Percoll: GE Healthcare, 17-5445-02 with 10% 10x PBS) in Medium A ...
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bioRxiv - Immunology 2023Quote: ... Cell lysates and samples were separated by standard SDS–PAGE or Tricine-SDS-PAGE (for HCMV US18/US20 blots) and transferred to PVDF membrane (GE Healthcare) using wet transfer in Towbin blotting buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... MNCs were isolated from each sample using the standard gradient separation approach with Ficoll-Paque PLUS (GE Healthcare Lifesciences, Pittsburgh, PA).
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bioRxiv - Cell Biology 2023Quote: GST and GST-DysSR22-23 were produced in BL21 bacteria using a standard protocol and were purified on Glutathione Sepharose 4B (GE Healthcare) [55] ...
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bioRxiv - Plant Biology 2024Quote: ... gel containing 7 M urea and buffered with 0.5 X TBE using an SE600 standard dual-cooled gel electrophoresis unit (GE Healthcare, USA) until the bromophenol blue dye reached the bottom of the gel ...
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bioRxiv - Biochemistry 2024Quote: ... Origin substrates were amplified using standard PCR protocols and were purified by anion exchange chromatography using a 1 ml RESOURCE Q column (GE healthcare). Peak fractions were ethanol precipitated and resuspended in TE buffer.
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bioRxiv - Developmental Biology 2021Quote: ... and Protein G Mag Sepharose (GE healthcare). The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein A-agarose beads (GE Healthcare, USA) were incubated with the supernatant at 4 °C for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: The beads (G protein Sepharose, GE Healthcare) were washed twice in IP buffer and centrifuged for 2 min at 2’700 x g before blocking with 1 mg/ml BSA in IP buffer for 1 h ...
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bioRxiv - Physiology 2021Quote: ... Protein G Sepharose was from GE Healthcare. Rhodocytin and collagen related peptide (CRP ...
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bioRxiv - Biochemistry 2022Quote: ... and protein A affinity chromatography (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... together with protein A Sepharose (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... Protein A Sepharose (GE Healthcare cat.# 45002982) and Protein G Sepharose (GE Healthcare cat.# 45000118 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose (50 µl, GE Healthcare) was added to the hippocampal lysates and the mixture was rotated at 4°C for 60 min ...
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bioRxiv - Genetics 2019Quote: ... Proteins were detected with ECL (GE Healthcare) using a digital imaging system Image-Lab (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hrs at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies (antibodies listed in Supplementary Table 1) ...
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bioRxiv - Cell Biology 2021Quote: ... or agarose Protein G beads (GE Healthcare) only ...
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bioRxiv - Molecular Biology 2022Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hours at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... or Protein A HRP (GE Healthcare, NA9120V), all at 1:2500 dilutions (Leng ...
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bioRxiv - Cell Biology 2023Quote: ... G-protein coupled sepharose beads (GE Healthcare) were pre-washed in lysis buffer and blocked overnight in the lysis buffer with 1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Protein-DNA immunocomplexes were retrieved by incubation with 60 µl of equilibrated protein G-conjugated agarose beads (GE Healthcare), for 2 h at 4⁰C ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated with a rabbit polyclonal anti-HDA15 antibody and protein G Mag Sepharose beads (GE Healthcare) overnight at 4°C ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
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bioRxiv - Cancer Biology 2022Quote: ... All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). Further details can be found in our previous publication [14].
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and soluble HA trimers were purified from transfected cell supernatants by standard Ni-NTA chromatography using a prepacked HisTrap™ HP column (GE Healthcare) and SEC using a HiLoad® 16/600 Superdex® 200 column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs) using Biacore 8000 (GE Healthcare) and a running buffer of composed of 20mM PB7.4 with 300 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Biophysics 2019Quote: The proteins and protein complexes were analyzed by analytical gel filtration using a Superdex 200 5/150 column (GE Healthcare) and the 1260 Infinity Bio-inert high-performance liquid chromatography system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...