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Citations for GE Life Sciences :
151 - 200 of 6111 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the mAb was purified using a 1 mL HiTrap Protein A HP column (GE Healthcare). The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and protein was eluted by ca 40 units PreScission protease (GE Healthcare) for 4 hours at 4°C under rotation ...
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bioRxiv - Cell Biology 2021Quote: ... and then for another 1 h following the addition of protein-G Sepharose (GE Healthcare). Protein bound to antibody beads was washed five times with lysis buffer at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The ITC protein sample p52 (1-398) went through desalting column (HiTrap desalting, GE Healthcare) to freshly made ITC buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2020Quote: ... using multiple runs over a 1 ml Protein-A HiTrap column (GE Healthcare Life Sciences) on an ÄKTA FPLC (GE Healthcare Life Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 1 h of incubation with protein A–Sepharose CL-4B beads (GE Healthcare). Immunoprecipitates were washed three times in lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... The protein solution was loaded on to a 1 ml Mono S column (GE Healthcare), eluted with a 20-bed volume linear gradient from 0 to 500 mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM Human DDX5 siRNA pool (GE Healthcare Dharmacon ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Biochemistry 2019Quote: Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... protein G-Sepharose or protein A-Sepharose beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: cDNA encoding human Nup35 was amplified from MGC Human Nup35 sequence-verified cDNA (Accession number BC047029, GE Healthcare, Dharmacon), using primers that add an amino terminal HA tag ...
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bioRxiv - Molecular Biology 2022Quote: ... the PCR amplified eleven positive virus genomes were amplified by RCA (rolling circle amplification) using the RCA-based TempliPhi DNA amplification kit (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
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bioRxiv - Cancer Biology 2023Quote: WDR5 (residues 22-334 or 1-334) and PTENα (residues 1-576) proteins were further purified using a Superdex200 gel-filtration column (GE Healthcare, 28989335) with a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... lysed protein was purified by cation exchange using a 1 mL HiTrap SP HP (GE Healthcare) in protein buffer (50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Systems Biology 2020Quote: ... Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare) to remove free His-tagged GST and TEV as well as remaining impurities ...
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bioRxiv - Molecular Biology 2020Quote: ... and POLGARF bound proteins were eluted by incubation with 1 μl of Prescission Protease (GE Healthcare) in PBS at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein was purified using a 1 ml HisTrap FF column (GE Healthcare, Amersham, United Kingdom) with imidazole gradient elution ...
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bioRxiv - Bioengineering 2020Quote: ... The collected supernatant was purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... pH 7.0) and loaded to a 1-ml HiTrap Protein G Sepharose FF column (GE Healthcare). The column was washed with binding buffer until the UV280 absorbance dropped to a stable baseline ...
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bioRxiv - Microbiology 2021Quote: ... pH 5.0 immediately before purification on a HiTrap Protein G HP 1 mL column (GE Healthcare) using an AKTA pure instrument ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT) and bound protein was eluted directly onto a HiTrap Heparin column (GE Healthcare) using buffer Hep A supplemented with 2.5 mM Desthiobiotin ...
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bioRxiv - Microbiology 2022Quote: ... Filtered lysates with strep-tagged proteins were applied onto StrepTrap HP 1 ml columns (GE Healthcare). Subsequent protein separation was conducted using the ÄKTA start chromatography system (GE ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred (100 V, 1 hr) onto PVDF (polyvinylidene difluoride) membranes (GE Healthcare Life Sciences) using 1X Tris/Glycine buffer (in-house 50X solution containing 250 mM Tris and 1920 mM Glycine) ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were loaded on a 5 ml HisTrap™HP column (1 mL/min; GE Healthcare) in a binding buffer containing 100 mM tris-HCl pH 8 ...
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bioRxiv - Immunology 2022Quote: ... Th gB-1 mRNA was purified by fast protein liquid chromatography (FPLC; Akta purifier; GE Healthcare)79 ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Synthetic Biology 2023Quote: ... Recombinant proteins were then purified by 1 mL Ni Sepharose (17-5318-01, GE Healthcare, USA). Columns were sequentially washed with 20 mL buffer 1 and 10 mL buffer 2 (in mM ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare) for immunoblotting ...
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bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.
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bioRxiv - Cell Biology 2021Quote: ... and proteins were collected on protein G-Sepharose beads (GE Healthcare) for an additional 4 h at 4°C with gentle shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... a mixture of protein A and protein G sepharose (GE Healthcare life sciences ...
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bioRxiv - Developmental Biology 2021Quote: ... nitrocellulose Hybond-C extra (GE Healthcare) membranes were spotted with 0 to 200 μM of PA (Sigma P-9511 ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 1 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Biochemistry 2019Quote: Human BRG1 AT-BD (residues 1434–1569) or BD (residues 1454-1569) were cloned into a pGEX6p-1 vector (GE Healthcare) from full length BRG1 cDNA obtained from GE Open Biosytems ...
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bioRxiv - Microbiology 2020Quote: ... each well in the plate was further incubated with the HRP-sheep anti-mouse or anti-human secondary antibody (1:5000, GE Healthcare) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Cell Biology 2020Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over Nap10 (GE Healthcare, 17-0854-01) and PD10 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over NAP- 10 (GE Healthcare, 17-0854-01) and PD-10 (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...