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Citations for GE Life Sciences :
151 - 200 of 2716 citations for 3 Acetyl 5 4 chlorophenyl 2 methylfuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and 2 mM DTT and loaded on a 5 ml HiTrap™ Phenyl Sepharose High-Performance column (GE Healthcare, Munich, Germany). The sample was eluted with a 1.0 to 0.4 M linear gradient of (NH4)2SO4 for 8 CV at a flow rate of 1 ml.min-1 ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Plant Biology 2024Quote: ... at a 1 to 20 ratio in Buffer A overnight at 4°C followed by a subtractive His trap purification using a 5 mL HisTrap HP column (GE Healthcare, Little Chalfont, UK) (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) and peptides focused for 20 kVh ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) diluted 1:50 in 5% glycerol ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was cleaved with Precission™ protease for 2 hours at room temperature and applied to a 5 mL HiTrap Q HP column (GE Healthcare). Protein was eluted from the Q HP column with a 2M NaCl buffer in a 100 mL gradient ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 500 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA FPLC (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3 mL fractions ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 250 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA Purifier (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3-ml fractions ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Immunology 2024Quote: ... to 2 ml and subsequently loaded on a Sepharose 4 Fast Flow resin column (10 mm x 300 mm; GE Healthcare, Little Chalfont, UK), connected to the ÄKTA pure system (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... 17µl (∼ 5 µg) of each Cy-5-labelled (GE Healthcare) cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...
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bioRxiv - Immunology 2024Quote: ... IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare, Life Sciences) dependent on culture volume ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted peak fractions were loaded at 2 mL/min in Buffer A onto a 5 mL HiTrap Heparin column (GE Healthcare Life Sciences) and eluted at 2 mL/min with a 50 mL gradient from 30% to 100% Buffer B ...
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bioRxiv - Microbiology 2021Quote: ... or pGEX-6P-3 (GE Healthcare). Pc3566 (from the −35 box to the TSS ...
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bioRxiv - Microbiology 2023Quote: ... 1.04% PharmalyteTM 3 – 10 (GE Healthcare), 50 mM DTT and trace amounts of bromophenol blue) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 % glycerol into two 5 mL MBP-trap cartridges (GE Healthcare) connected in tandem before removal of the Ni-NTA cartridges ...
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bioRxiv - Cell Biology 2020Quote: ... The mixture was incubated for 2 hours at RT and antibodies were isolated using illustra™ NAP-5 columns (GE Healthcare Life Sciences™), resulting in an average labeling rate between 0.8 and 1.
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated in Buffer B ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was then filtered through a 0.2 µm syringe filter before being loaded onto 2 x 5 mL StrepTrap HP Columns (GE Healthcare Life Sciences 28-9075-47), equilibrated in Step A Buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... 3 ml Ficoll-Paque media (GE Healthcare) was pipetted into the bottom and the diluted 4 ml blood sample was carefully layered on top ...
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bioRxiv - Neuroscience 2023Quote: ... pH 3–10 (GE Healthcare Life Sciences), and bromophenol blue were added and centrifuged (14,000 rpm for 10 min ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
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bioRxiv - Biochemistry 2023Quote: ... Glutathione-sepharose 4 beads (GE Healthcare) were added to remove the cleaved tags and non-cleaved protein for another 2 h at 4°C in a column under gravity flow ...
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bioRxiv - Microbiology 2024Quote: ... 2 µMnt ssDNA (Ovio 45) and 2 mM ATP (GE Healthcare) in a 40 µL reaction solution containing 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... diluted 1:10,000 in 2% TBST with 2% Blocking (GE Healthcare), was applied for 1 h.
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bioRxiv - Biochemistry 2022Quote: ... and then immobilized in 7 cm IPG strips (pH 3-10 : 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG ...
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bioRxiv - Immunology 2024Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0–0.5 M NaCl in 20 mM Tris (pH 8.0) ...
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bioRxiv - Immunology 2024Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0-0.5 M NaCl in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... [3] A HisTrap FF column (Cytiva, GE Healthcare) was used in the Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... Cy®5 (GE Healthcare) anti-mouse secondary antibody was used ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% FBS (Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30-60 min at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% FBS (Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30 min at 37°C and 120 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... and the cleaved DRH-3 CTD and DRH-3 NTD were purified using a Superdex 75 HiLoad 16/600 size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 5-15 mg ml−1 in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sample was then run on an 18 cm 3-10 strip using the Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare). Four voltage steps (50 V for 10 hours ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluate was further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were pooled and protein purity was assessed by SDS–PAGE and Coomassie staining ...