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Citations for GE Life Sciences :
151 - 200 of 2374 citations for 1 4 Bromo 3 methylphenyl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Cells were then disrupted by sonication and after centrifugation (30 min at 30000 × g at 4°C) the supernatant (soluble fraction) was purified by FPLC on a 1 ml HisTrap FF column (GE Healthcare; Milan, Italy) by stepwise elution ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated at 4°C for at least 1 hr with ∼40 µL of appropriate bead slurry (nProtein A Sepharose, GE Healthcare, 5280-01). Following incubation ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2024Quote: The OVA protein was first attached to an oligonucleotide using a succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) linker and purified using a NAP column (GE Healthcare Life Sciences). Unconjugated oligonucleotides were removed by 30K Amicon filtration (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mm x 1 mm x 1 mm) and FDG-PET data acquisitions were performed simultaneously on a hybrid 3 T SIGNA™ PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the Service of Nuclear Medicine (CUB Hôpital Erasme ...
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bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared at 26,200g for 30 min at 4°C and supernatants were incubated with pre-washed Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 90 min at 4°C with mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was supplemented with 4 mM CaCl2 and stirred for 2 h in 4°C with CaM-Sepharose beads (GE Healthcare), pre-equilibrated with binding buffer (50 mM Tris/HCl pH 7.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
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bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
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bioRxiv - Developmental Biology 2020Quote: NHS (N-hydroxysuccinimide) activated Sepharose 4 fastflow (GE Healthcare) beads coupled with an RNA aptamer alone control or aptamer fusion (zebrafish ndr1 3’UTR sequences fused to a Tobramycin aptamer ...
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bioRxiv - Molecular Biology 2021Quote: ... and Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads by dimethyl pimelimidate cross-linking as described in (69).
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bioRxiv - Molecular Biology 2021Quote: ... nProtein A Sepharose 4 Fast Flow beads (GE Healthcare) were used ...
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bioRxiv - Biochemistry 2022Quote: ... and Glutathione SepharoseTM 4 Fast Flow beads (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... or Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) following the instructions provided by the manufacturer with appropriate modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... with 4 µl of Cy5 NHS Ester (GE Healthcare; 1 mg dye dissolved in 10 µl of anhydrous DMSO ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μL were spotted onto dry nitrocellulose (GE Healthcare) in 15-second increments ...
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bioRxiv - Genetics 2023Quote: ... or ProteinG SepharoseTM 4 Fast Flow (GE Healthcare, 17061801). The crosslinking was reversed ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C onto 0.2uM PVDF membranes (GE Healthcare Biosciences). After transfer ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2020Quote: ... cDNA constructs were inserted into the pGEX6P-3 plasmid (GE Healthcare) and expressed as GST-fusion proteins in High efficiency BL21(DE3 ...
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bioRxiv - Cell Biology 2022Quote: Early passage HUVECs were coated on Cytodex-3 beads (GE Healthcare) at a density of 10 million cells/40 μl beads and incubated in suspension for 3-4 hours with gentle mixing every hour ...
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bioRxiv - Molecular Biology 2024Quote: ... and applied to 3 × 5-mL StrepTrap HP columns (GE Healthcare), connected in series ...
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bioRxiv - Immunology 2021Quote: Total IgG was initially purified from 4 mL of the multigravidae plasma pool using Protein G Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The pellet was re-suspended in 4 ml PBS prior to layering over 4 ml of Ficoll solution (GE Healthcare life sciences, UK) in a 15 ml centrifuge tube and centrifuged at 1400 x g for 45 minutes ...
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bioRxiv - Pathology 2024Quote: Cardiac MRI was performed prior to treatment at baseline (4 weeks post-MI) and at 4 weeks post-treatment on a 3T Premier scanner (GE Healthcare, Waukesha, WI). Specifically ...
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bioRxiv - Plant Biology 2022Quote: Leaf samples from ino-4 and ino-4 i20 plants were separately pooled and DNA extracted with Nucleon Phytopure DNA extraction kit (GE Healthcare, Pittsburgh, PA), according to the provider’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins from input and eluates were separated on 4-20% or 4-18% home-made SDS-PAGE and transferred onto PVDF membranes (GE Healthcare, Chicago, Illinois, USA) followed by detection using HRP conjugated primary anti-HA (clone 3F10 ...
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bioRxiv - Immunology 2020Quote: ... and 0.1 M 3-(N,N-dimethylamino) propyl-N-ethylcarbonadiimide (GE healthcare) at a flow rate of 5 µL/min ...
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bioRxiv - Microbiology 2021Quote: ... dadantii OutB was cloned into the pGEX-6P-3 vector (GE Healthcare) encoding a cleavable N-terminal Glutathione S-transferase (GST ...
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bioRxiv - Cancer Biology 2024Quote: ... for 3-5 minutes and visualized using Amersham Imager 600 (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... using a multi-frequency linear array transducer (3-12 MHz, GE Healthcare). Participants were instructed to lay on an athletic training table in the supine position 5 minutes prior to imaging ...
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bioRxiv - Cell Biology 2024Quote: ... 3D-SIM was carried out using an OMX Version 3 (Applied Precision) microscope equipped with a UPlan APO 100× 1.4 NA oil-immersion objective (Olympus) ...
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bioRxiv - Biochemistry 2022Quote: ... and 3 mM MgCl2] using a size exclusion column (NAP5; GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... on Protein G Sepharose 4 Fast Flow beads (GE healthcare). The samples were incubated at 4°C overnight and then washed with progressively more stringent conditions ...
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bioRxiv - Biophysics 2021Quote: ... at 4°C using AKTA Pure HPLC system (GE Healthcare). The column was washed with 20 mM NaPi (pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... and Glutathione Sepharose 4 Fast Flow (GE Healthcare Life Sciences), respectively ...