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Citations for GE Life Sciences :
1801 - 1850 of 5568 citations for Human IgM Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The dialyzed protein extract was purified via a His-trap column (GE Healthcare) previously equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was loaded on a SP column (from GE healthcare Life sciences) and eluted by applying a NaCl gradient (from 0.15 to 1 M) ...
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bioRxiv - Cell Biology 2020Quote: ... The eluted protein was fractionated using a Superose10 gel filtration column (GE Healthcare). Protein concentrations were determined using a NanoDrop 1000 spectrophotometer (Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The GST-TTD fusion protein was purified using Glutathione sepharose 4B (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and the protein was bound to a HiTrap heparin HP column (GE Healthcare), before elution with a gradient from 335 mM to 1 M KCl ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 µL of Protein A Sepharose 4 FF (GE Healthcare Japan, Tokyo, Japan) was added and mixed with rotation at 4°C for 3 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Protein purification was performed using a 5 ml StrepTrap HP column (GE Healthcare) followed by SEC using a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were purified sequentially by Ni-NTA affinity chromatography (HisTrap FF, GE Healthcare), heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Biochemistry 2021Quote: ... the expressed proteins were first purified by Glutathione Sepharose 4B beads (GE Healthcare), and then further purified with a HiLoad 16/600 Superdex 200 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was separated using a second HisTrap HP column (GE Healthcare). His6-tag free Ape1 protein was then loaded onto a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Phosphorylated proteins were recovered by gel filtration on Superdex 200 Increase (GE Healthcare) in 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were initially purified using Ni Sepharose 6 Fast Flow resin (GE Healthcare). The protein tags were cleaved with His-tagged TEV-protease overnight at 4 °C while dialyzing against TEV cleavage buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Purified recombinant proteins were loaded onto Superdex 75 10/300 column (GE Healthcare) in 150mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Buffer exchanged protein was applied to a 5mL HiTrap QHP column (GE Healthcare,) using a 30% gradient of QB buffer (20mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated and loaded onto a Superose 6 column (GE Healthcare) pre-equilibrated in purification buffer and peak fractions were pooled and concentrated to 5-6 mg/mL.
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bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were visualized by chemiluminescent detection (ImageQuant LAS 4000, GE Healthcare Life Sciences) of peroxidase substrate activity (SuperSignal West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on SDS– polyacrylamide gel were transferred onto a nitrocellulose membrane (GE Healthcare). The bands were visualized utilizing Enhanced Chemiluminescence (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... and purified by fast protein liquid chromatography using Calmodulin-Sepharose (GE Healthcare, USA). TAT-CaM was expressed in BL21(DE3)pLysS E ...
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bioRxiv - Cell Biology 2021Quote: ... GST-fusion proteins were purified by incubation with glutathione-Sepharose beads(GE Healthcare). The precipitates were washed 3X with lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... Excess dye was removed by purifying protein over NAP-5 columns (GE Healthcare). Labeling was confirmed using NanoDrop 2000c (Thermo Fisher).
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified by Ni2+-IMAC on HisTrap columns (GE Healthcare). Fractions ...
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bioRxiv - Microbiology 2020Quote: ... Spike protein was finally purified using Superose 6 HR10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES (pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... the protein solution was applied to a HiTrapTM Q HP column (GE Healthcare) and eluted with linear gradient of 0.15-2.0 M of NaCl in 10 mM Tris (pH 8.0) ...
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bioRxiv - Immunology 2020Quote: ... The recombinant GST fusion proteins were affinity purified on GSH-Sepharose (GE Healthcare) from bacterial cultures incubated with 0.25 mM isopropyl-β-D-thiogalactopyranoside (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... mRNA was purified by Fast Protein Liquid Chromatography (FPLC) (Akta Purifier, GE Healthcare), as described 74 ...
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bioRxiv - Immunology 2021Quote: ... by incubating sera with a resin of protein G Sepharose beads (GE Healthcare) for 2h ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by gel filtration chromatography (Superdex-200, GE Healthcare) with columns equilibrated in storage buffer (50 mM Tris-HCl 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to nitrocellulose membranes (GE Healthcare Life Sciences – REF 10600003), which were incubated with the appropriate antibodies according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant proteins were purified with a fast flow Ni-NTA column (GE Healthcare) and eluted by 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biophysics 2020Quote: ... the reduced protein was bound to a Ni2+-Sepharose resin (GE Healthcare, UK) and washed with 10 column volumes of 50 mM KPi (pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were wet transferred to nitrocellulose membrane (0.45 μm, GE Healthcare Life Sciences) at 100 V for 75 min ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were visualized using the ImageQuant LAS500 chemidocumenter (GE Healthcare, Chicago, USA). The ECL signal was visualized in the chemiluminescent channel ...
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bioRxiv - Cancer Biology 2022Quote: ... Buffer replacement in protein purification used Column PD 10 desalting column (GE Healthcare). All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were purified on a Ni-NTA HiTrap HP column (GE Life Sciences) and the His tag was removed by overnight cleavage with TEV protease (homemade) ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was desalted using a PD10 column (GE Healthcare, Cat. no. 17085101) in 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... NQO1 proteins were purified using immobilized nickel affinity chromatography columns (IMAC, GE Healthcare) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... Tagged proteins were purified by affinity chromatography (HisTrap resp. StrepTrap, both GE Healthcare) with elution buffer containing 200 mM imidazole respectively 2.5 mM d-desthiobiotin ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified by SEC on a Superdex 75 (GE Healthcare) column ...
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bioRxiv - Microbiology 2022Quote: ... The dimeric ACE2 protein was purified by GammaBind Plus Sepharose beads (GE Healthcare), followed gel filtration chromatography on a Superdex 200 Increase 10/300 GL column ...
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bioRxiv - Microbiology 2022Quote: ... Materials and equipment for protein purification were obtained from GE Healthcare (Munich, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... GST fusion proteins were immuno-precipitated using Glutathione Sepharose 4B beads (GE Healthcare). Fusion protein-bound beads were used directly in GST pull-down assays ...
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bioRxiv - Microbiology 2022Quote: ... protein was loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) with TBS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins separated on gels were electroblotted onto nitrocellulose (Protran 0.2 μm, GE Healthcare) with a constant current of 100 mA for 10 h using wet transfer ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were precleared by incubating with protein G-Sepharose beads (GE Healthcare), and reacted with 100 μL of Strep-Tactin Sepharose beads (IBA Lifesciences ...
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bioRxiv - Molecular Biology 2022Quote: ... on an ÄKTA explorer fast protein liquid chromatography (FPLC) system (GE Life Sciences) in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... were applied along with Protein G Sepharose 4 Fast Flow beads (GE Healthcare). All ChIP extracts were pre-cleaned for 1 hr with 30 μl beads on a rotor at 4°C in absence of the antibody ...
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bioRxiv - Immunology 2022Quote: ... The separated proteins were transferred on Amersham Protran nitrocellulose membrane (GE Healthcare Lifescience). After blocking with 5 % skimmed milk in TBS-T (0.1 % Tween-20 (Sigma-Aldrich ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...