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Citations for GE Life Sciences :
1801 - 1850 of 3098 citations for Anti Cullin 5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The dialyzed samples were applied to a 5 ml HiTrap Q HP anion exchange column (GE Healthcare Life Sciences). The Q anion exchange column was eluted via linear gradients from 0% to 100% elution buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2022Quote: ... The soluble fraction was loaded onto a 5 mL Ni-NTA column connected to an ÄKTA pure (GE Healthcare) chromatography system ...
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bioRxiv - Neuroscience 2022Quote: ... The column was washed with 10 column volumes of Buffer A (10 mM HEPES, pH 7.3, 0.15 M NaCl, 5 mM imidazole) on an Aktapurifier (GE Healthcare) while collecting fractions ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysate was then 0.45 μm filtered and applied to a 5 mL HisTrap HP column (GE Healthcare). The column was washed with five column volumes of wash buffer ...
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bioRxiv - Biophysics 2023Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5 ml column (GE Healthcare, USA) for cation exchange ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µM Drp1 was injected onto a Superose 6 10/300GL column in an ÄKTApure FPLC system (GE Healthcare) connected in line with DAWN Heleos-II 18-angle MALS and Optilab T-rEX differential refractive index (dRI ...
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bioRxiv - Biochemistry 2023Quote: Dimer formation of non-farnesylated GBP1 was analyzed by analytical size-exclusion chromatography (Superdex 200 5/150, GE Healthcare) as described (Ince et al ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Cell Biology 2023Quote: ... PARP14 MD1 was additionally purified by ion exchange chromatography using a HiTrap™ 5 mL SP HP (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed 5 more times and the signal was detected by chemiluminescence using ECL Prime (GE Healthcare) on an Amersham Imager 600 detection system (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... Membranes were exposed to X-ray for 5-10 min using a biomolecular imager (ImageQuant LAS4000, GE Healthcare, USA). Relative cytokine content was determined after quantification of the dot intensity using ImageJ.
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (1-5 mg/ml) were loaded individually on a Superdex 200 Increase 10/300 column (GE Healthcare), and the elution profiles were obtained using a tandem of sequentially connected UV-Vis Prostar 335 (Varian ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered (0.45µm) and loaded onto a Ni2+-affinity column (HisTrap FF, GE Healthcare 5 mL column) equilibrated in low imidazole buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... supernatant filtered through 0.45 µm syringe filter and loaded onto a HiTrap Q HP column (5 ml, GE Healthcare) that was stacked on top of a SP column (5 ml ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE using 4% or 5% polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA ...
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bioRxiv - Bioengineering 2023Quote: ... The flowthrough sample was collected and loaded onto the pre-equilibrated HiTrap SP HP 5 mL column (GE Healthcare) with a flow rate of 2.5 mL/min ...
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bioRxiv - Biophysics 2023Quote: ... Data collection was carried out at 20 °C using a Superdex200 Increase 5/150 analytical SEC column (GE Healthcare) equilibrated in the appropriate buffers (see Table S3 ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins in the soluble fraction were purified by Ni-affinity column chromatography (HisTrap 5 ml column, GE Healthcare). The His6-tag was removed by thrombin digestion (1 μg of thrombin per mg of TelSi3 protein ...
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bioRxiv - Biochemistry 2024Quote: ... uncleaved protein as well as cleaved GST were removed by applying everything onto 5 ml SP column (GE Healthcare) equilibrated with 20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Biophysics 2023Quote: ... The TSP was then circulated through a pre-equilibrated (buffer A) Ni-NTA column (HisTrap, 5 ml, GE HealthCare) for 4 h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Microbiology 2024Quote: ... 30 min) and loaded onto a 5 ml HighTrap MBP column (Cytiva) using an Äkta-Pure system (GE Healthcare). The column was washed with 10 column volumes of lysis buffer and protein eluted with MalE-elution buffer (100 mM Tris-HCl pH7.2 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed with TBST three times and incubated with sheep anti-mouse or donkey anti-rabbit IgG conjugated with horseradish peroxidase (HRP) (1:4,000, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... horseradish peroxidase (HRP)-linked anti-mouse IgG (from sheep) and HRP-linked anti-rabbit IgG (from donkey) (both GE Healthcare) were used as secondary antibodies.
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bioRxiv - Cell Biology 2019Quote: ... Peroxidase-linked anti-mouse immunoglobulin G and peroxidase-linked anti-rabbit immunoglobulin G (both GE Healthcare Bio-Sciences, Pittsburgh, PA). Alexa Fluor 594-conjugated Donkey Anti-Mouse IgG (1:250 ...
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bioRxiv - Biochemistry 2023Quote: ... Both anti-His and anti-Strep immunoblots were developed using Amersham ECL immunoblotting reagents (GE Healthcare Life Sciences, Buckinghamshire, UK).
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse, 1/3.000, #NA9310, GE Healthcare). An immune signal was detected using Clarity Western ECL substrate (#1705061 ...
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bioRxiv - Biochemistry 2020Quote: ... The antibody ligands were captured on a Protein A chip (GE Life Sciences) and human CD45 D1-D4 (amino acids 194-578 ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody-treated samples were next incubated with Protein A-Sepharose beads (GE Healthcare) at 4 °C for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound antibodies were detected with the Enhanced Chemiluminescence (ECL) system (GE Healthcare Amersham) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... horseradish peroxidase-linked secondary antibody (donkey monoclonal, 1:2000, GE Healthcare, Bucks, UK) for 45 min ...
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bioRxiv - Immunology 2021Quote: ... C monoclonal antibody (W6/32) coupled Sepharose-4B resin (GE Healthcare, IL, USA) at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibodies were detected using ECL™Western Blotting Detection Reagents (RPN2209, GE Healthcare). The expression of vinculin (rabbit anti-vinculin ...
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bioRxiv - Molecular Biology 2019Quote: ... The HRP-conjugated secondary antibodies (NA9340 and NA9310) were purchased from GE Healthcare Life Sciences and used at 1:5000 dilution.
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bioRxiv - Pathology 2019Quote: ... The antigen-antibody reaction was detected by ECL incubation (GE Healthcare Life Sciences) for 5 minutes on Licor C-DiGit Blot Scanner ...
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bioRxiv - Microbiology 2019Quote: ... Horseradish peroxidase-conjugated secondary antibodies (1:5000) and enhanced chemiluminescence reagents (GE Healthcare) were used to detect the bands on a Bio-Rad ChemiDoc MP system.
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bioRxiv - Immunology 2021Quote: Serum IgG antibodies were purified by protein G column chromatography (GE Healthcare, USA). The purified IgG antibodies were detected by SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... HRP-conjugated secondary antibodies signal was revealed using ECL Prime kit (GE Healthcare) and captured using the ChemiDoc imager (BioRad ...
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bioRxiv - Cancer Biology 2021Quote: ... HRP-linked secondary antibodies (NAV 930 and 931) were purchased from GE Healthcare UK Limited ...
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bioRxiv - Cancer Biology 2021Quote: ... PON1 monoclonal antibody was coupled to protein G-Sepharose 4B beads (GE Healthcare) and eluted as described previously[69] ...
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bioRxiv - Cancer Biology 2020Quote: ... Horseradish peroxidase-conjugated α-mouse antibody (1:10,000; NA931V, GE Healthcare, IL, USA) was used as a secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were then exposed to horseradish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare) diluted in blocking buffer for 60 min at RT and washed again ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were then exposed to horseradish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare) diluted in blocking buffer for 60 min at RT and washed again ...
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bioRxiv - Systems Biology 2020Quote: ... Chemical luminescence from the second antibody reacted with ECL prime reagent (GE Healthcare) was detected by a lumino image analyzer ...
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bioRxiv - Cell Biology 2020Quote: ... and thence with suitable HRP-conjugated secondary antibodies (GE Healthcare NA931V and NA934V) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... Horseradish peroxidase (HRP)- conjugated secondary antibodies and an electrochemiluminescence system (GE Healthcare, UK) were used to detect specific proteins ...
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bioRxiv - Cancer Biology 2021Quote: ... The KD value of each antibody was calculated using Biacore 8K (GE Healthcare) analysis software ...