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Citations for GE Life Sciences :
1801 - 1850 of 2418 citations for 8 9 Didehydro 3'N demethyl 9 deoxo 4'' 6 12 trideoxy 6 9 epoxy 3'N ethylerythromycin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Afterwards the lysates were subjected to SDS-polyacrylamide gel electrophoresis using an 8% polyacrylamide gel and then electroblotted onto a nitrocellulose membrane with a pore size of 0.45 μm (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... beads were washed once in lysis buffer and rotated at 4ºC overnight in wash buffer containing 8 U/ml thrombin (GE healthcare) to remove the GST tag ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were separated by electrophoresis on an 8% polyacrylamide gel and then transferred onto a nitrocellulose membrane (GE Healthcare) for 90 minutes at 100V ...
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bioRxiv - Microbiology 2023Quote: ... elution in buffer A plus 350mM imidazole (pH 8) and gel filtration chromatography on a Superdex 200 column (GE Healthcare) in buffer A.
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were further enriched by centrifugation at room temperature at 695 g for 8 min in 37.5% Percoll (GE Healthcare), prior to washing and resuspension for downstream analysis.
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 18000-20000 rpm at 4 °C and supernatant was allowed to bind to Glutathione resin (GS resin) (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat no. 17-5132-02) in a batch binding mode for overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose 4 Fast flow beads (GE Healthcare Life Sciences) washed twice with wash buffer were added to each IP and incubation was continued for 2 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... and Protein G Sepharose 4 Fast Flow Media (#11524935, GE Healthcare) to pull-down phosphorylated IRF5 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NHS-activated Sepharose 4 Fast Flow was obtained from GE Healthcare and protein ladder from Thermo Fisher Scientific.
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bioRxiv - Cancer Biology 2019Quote: ... 30 µL of protein G sepharose 4 fast flow (GE Healthcare) was bound to 5 µL of anti-HN1 (GeneTex ...
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bioRxiv - Biochemistry 2019Quote: ... GluC was removed with benzamidine Sepharose 4 Fast Flow (GE Healthcare) resin ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant was incubated with Gluthathione Sepharose 4 Fast Flow (GE Healthcare) for 2 hours at 4 °C ...
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bioRxiv - Genetics 2021Quote: ... Glutathione Fast Flow Sepharose 4 beads (GE Healthcare, 17-5132-01) that had been pre-washed in binding buffer were then added to the binding reaction and it was incubated in a rotor for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose 4 Fast Flow (17-0618-01, GE Healthcare), Dynal magnetic beads (110.31 ...
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bioRxiv - Biophysics 2023Quote: ... and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare), and Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Bioengineering 2024Quote: ... at 4 °C using an ÄKTA Pure 25 M (GE Healthcare). The column was washed with 75 mM imidazole [in 20 mM NaH2PO4 and 0.5 M NaCl (pH 7.4)] ...
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bioRxiv - Biophysics 2021Quote: ... The TLC plate was exposed to Fuji film BAS-MS 2025 imaging plate for 12 h and analyzed using Typhoon FLA-9500 (from GE Healthcare). The background obtained from reaction buffer (having no EccA1 and (γ32P ...
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bioRxiv - Cell Biology 2020Quote: ... were acquired every 3 min for 12 h with a 40× oil-immersion 1.3 NA objective using an Olympus DeltaVision Elite microscope (Applied Precision, LLC) equipped with a CoolSNAP HQ2 camera (Roper Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... proteins (10 μL each of lysate or wall fractions) were separated in a 12% polyacrylamide gel before transfer to nitrocellulose (GE Healthcare. For preparation of cell wall extracts for mass spec ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant CLASP2 C-term (300 μg, injection volume of 100 μl) was analyzed on a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated in buffer C ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was cleaved with 3C protease overnight and Nes2G-SR11/12 was further purified by cation exchange chromatography on a HiTrap SP-FF column (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: Approximately 500,000 cells were transfected using a final concentration of 20 nM EPSTI1 (J-015094-09-0020 and J-015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 µL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants of the cell lysates were run on 12% resolving Tris-glycine gels and then transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). To detect PstP-strep-tag II ...
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bioRxiv - Biochemistry 2022Quote: The oligomerization state and molecular mass determinations were carried out by Multiple-Angle Laser Light-Scattering (MALLS) using a miniDawn (Wyatt Technology) coupled to a size–exclusion column (SEC, Superose-12, GE Healthcare), at room temperature and at a 0.3-0.4 mL min−1 flow rate ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on a 12 % denaturing acrylamide gel and subsequently transferred onto a nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membrane was blocked using 1x Roti®Block (Carl Roth ...
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bioRxiv - Cell Biology 2022Quote: ... Cat# 015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 μL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Biophysics 2019Quote: ... Protein samples (each protein at a concentration of 40 μM) were loaded to a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with assay buffer (same with ITC).
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bioRxiv - Molecular Biology 2019Quote: ... Fractions containing the highest concentration of TASK-1 were pooled and concentrated to 12-30 mg/ml ml with a 50 kDa molecular weight cut off spin concentrator (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... Supernatants from the cell lysate were run on 12% resolving Tris-Glycine gels and then transferred onto PVDF membrane (GE Healthcare). Rabbit α-strep antibody (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of lysates containing Laemmli buffer were heated at 95°C and electrophoresed on 12% polyacrylamide gels under standard SDS-PAGE conditions before being transferred onto a polyvinylidene fluoride membranes (GE Healthcare). Protein loaded membranes were blocked with 5% (w/v ...
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bioRxiv - Immunology 2022Quote: ... Samples were resolved by SDS-12% polyacrylamide gel electrophoresis under standard conditions and transferred to a polyvinylidene difluoride (PVDF) membrane (Amersham Hybond, GE Healthcare) using the Trans-Blot turbo transfer system (Biorad) ...
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bioRxiv - Plant Biology 2022Quote: ... equal amounts of proteins were separated on a 10% or 12% SDS-polyacrylamide (PA) gel and transferred to nitrocellulose membranes (GE Healthcare). The signal was probed with specific antibodies using Clarity Western ECL Blotting Substrate (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... total protein extracts (10 to 20 μg) were subjected to 12% or 15% SDS-PAGE and then transferred to 0.45 μm nitrocellulose membranes (GE Healthcare, 10600003). The anti-CrATG3 polyclonal antibody was produced by injecting the recombinant wild-type CrATG3 protein into a rabbit using standard immunization protocols at the Animal Resource facility from the University of Sevilla ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant (post-vesicle fraction, PVF) was then fractionated with Superose 12 10/300 GL column (GE Healthcare, Little Chalfont, UK) (flow rate 0.4 mL/min ...
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bioRxiv - Microbiology 2023Quote: ... the recombinant proteins were purified from the supernatant using nickel-affinity chromatography (Ni-NTA agarose, ABT) followed by size exclusion chromatography (Superose 12 10/300 GL, GE Healthcare), except in the cases of AIP56N1-E307 and AIP56N1-E307DTR in which only nickel-affinity chromatography was performed ...
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bioRxiv - Cancer Biology 2023Quote: ... and separated by 4-12% Bis-Tris SDS-PAGE electrophoresis (at 180 V, 300 W, 55 min) and transferred onto a nitrocellulose membrane (GE Healthcare). Membranes were blocked in 5% BSA(Sigma ...
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bioRxiv - Immunology 2022Quote: ... Samples were resolved on 12–15% SDS–PAGE gels and then proteins were wet transferred to Protran nitrocellulose membranes (GE Healthcare) at 400 mA at 4 °C for 1.5 h in 1x Transfer buffer (25 mM Tris base ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were resolved by electrophoresis on 10 or 12% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (GE Healthcare). Total protein labeling was performed using No Stain Labeling Reagent (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... TCA extracts of total yeast or 2 μg of mitochondrial proteins were loaded on a 12 % SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare). UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD) ...
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bioRxiv - Plant Biology 2023Quote: Equal amounts of protein extracts were electrophoresed on 12% sodium dodecyl sulphate (SDS) polyacrylamide gel prior to transfer on Hybond ECL nitrocellulose membranes (GE Healthcare). Homogenous transfer was confirmed by Ponceau Red staining ...
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bioRxiv - Cell Biology 2023Quote: ... Reduced immunoprecipitated proteins were separated on a 10 or 12% SDS-polyacrylamide gel and transferred to a PVDF membrane (GE Healthcare). Details on the blocking of the membranes and detection of the proteins can be found in Table S1 ...
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bioRxiv - Biophysics 2021Quote: ... by injecting 50 µL of 8 µM protein in Protein Buffer onto a 1 mL HiTrap Heparin HP column (GE Healthcare) and eluting with a 10 CV linear gradient from 150 mM – 1 M NaCl in 20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
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bioRxiv - Biochemistry 2021Quote: ... and diluted with an equal volume of 50 mM Tris pH 8 before loading onto HiTrap Heparin HP columns (GE Healthcare). After extensive washing with 50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2019Quote: SEC-MALS was conducted using a Wyatt Dawn 8+ detector (Wyatt Technology) coupled to a Superdex 75 10/300 column (GE Healthcare) and a refractive index detector ...
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bioRxiv - Neuroscience 2020Quote: ... The lysates were centrifuged at 18000 rpm (F21-8×50y rotor) for 45 min and supernatants were purified by Ni2+-affinity chromatography (FPLC system ÄKTA Purifier, GE Healthcare) eluting the protein in 50 mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were separated by denaturing (8 M urea) 10% PAGE and were visualized by using a Typhoon phosphorimager (GE Healthcare). As described previously,48 band intensities were determined and used to estimate the fraction of RNAs bound to ligand ...
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bioRxiv - Biochemistry 2020Quote: ... the concentrated fractions containing ɸEL (eluting at 8–15 mS cm-1) were subjected to size exclusion chromatography (SEC) on Sephacryl S-400 (GE Healthcare) in buffer ...
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bioRxiv - Biophysics 2020Quote: ... eluate fractions from the Ni2+-NTA column containing the nsp7-8-His6 were concentrated and the buffer exchanged with a PD-10 desalting column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... Co-immunoprecipitants were resolved on an 8% SDS-PAGE gel followed by protein transfer on nitrocellulose membrane (GE Healthcare Life Sciences). Western blot analysis was performed as described above.