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Citations for GE Life Sciences :
1751 - 1800 of 2887 citations for Tubulin Delta 1 TUBD1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: FH was isolated from human plasma using an affinity column made up of a monoclonal antibody against human FH (MRCOX23; MRC Immunochemistry Unit, University of Oxford) coupled to CNBr-activated Sepharose (GE Healthcare, UK) as described previously (21) ...
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bioRxiv - Microbiology 2023Quote: ... Anti-rabbit immunoglobulin AP-conjugated secondary antibody was used and reaction results were obtained using CDP Start Detection Reagent kits (GE Healthcare, USA) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Results were normalized to the expression of β-actin (cat#: A3854, source: mouse, 1:80,000, Sigma-Aldrich; anti-mouse IgG (GE Healthcare; dilution: 1:25,000). Immunoreactive bands were visualized with an enhanced chemiluminescence detection system (ChemiDoc Imaging System ...
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bioRxiv - Microbiology 2020Quote: ... Enrichments were performed in test tubes containing 5 mL of M63 minimal media and a 1 x 10 cm strip of Whatman #1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA) pressed against the side of the tube (30) ...
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bioRxiv - Bioengineering 2023Quote: ... heated to 60°C to remove aggregates and further purified on two coupled IEX columns 1 mL HiTrap Q FF and 1 mL HiTrap SP FF (GE Healthcare Life Sciences, NSW, Australia) by collecting the flow-through ...
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bioRxiv - Molecular Biology 2023Quote: Fetal bovine serum and cosmic calf serum were mixed in a 1:1 ratio and incubated with pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... Blots were then washed and incubated with horseradish peroxidase-conjugated secondary antibodies and were developed using either ECL Prime (GE Healthcare, Piscataway, NJ) or WesternBright Sirius horseradish peroxidase substrate (Advansta) ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-Scp160 antibodies (Gift of R. Jansen, U. Tuebingen) was performed using the Amersham ECL Western Blotting Detection Kit (GE Healthcare Life Sciences). Quantification of protein bands was performed using ImageJ software.
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bioRxiv - Cell Biology 2021Quote: ... They were then incubated with a horseradish peroxidase-conjugated secondary antibody and proteins were detected using enhanced chemiluminescence (GE Healthcare Life Sciences, RPN2209). Densitometry analysis of bands was performed using Image J software.
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bioRxiv - Microbiology 2022Quote: ... Antibodies were detected using an ECL detection reagent and imaged on the ImageQuant LAS 4000 or Amersham Imager 600 (GE Healthcare Life Sciences). All quantitative analysis derived from western blotting was performed using densitometry analysis in ImageJ.
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: We used a modified version of a previously described protocol (41). Briefly, we immobilized monoclonal 1D4 antibody (generously provided by D. Oprian, Brandeis University) onto Sepharose-4B (GE Healthcare Life Sciences). All steps were done in a darkroom under dim red-light illumination ...
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bioRxiv - Molecular Biology 2019Quote: ... characterization of an N-terminal Aβ peptide DAE-EG (DAEFRHDSGYSGKQKSRNEGKGGC) binding to anti-mouse captured antibodies from hybridoma supernatants was performed using a BIAcore T-200 instrument (GE Healthcare, Marlborough, MA) [36] ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were affinity-purified from filtered cultured supernatant on a High-Trap protein G column (GE Healthcare, Chicago, USA, Cat.Nr 17-0404-01).
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated with horseradish peroxidase-coupled secondary antibodies and imaged using chemiluminescent substrate on an Amersham Imager 600 (GE Healthcare, Chicago, Illinois).
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed for 3x 5 minutes with TBST and incubated for 1 hour at RT with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG, Jackson ImmunoResearch 111-035-144 or sheep anti-mouse IgG, GE Healthcare NA931V) diluted 1:5000 in 5% milk / TBST ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... membranes were washed 3x in PBS-T and then incubated with appropriate secondary antibody conjugated with horseradish peroxidase (GE Healthcare, Chicago, IL, USA). Chemiluminescence was accomplished using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3 times 10 minutes each with TBS-T and then incubated with the corresponding IgG secondary antibodies horseradish peroxidase-conjugated (GE Healthcare Life Sciences) at 1:1000 for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies (anti-UBAP2L or anti-PLK1) as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Biochemistry 2023Quote: ... The antibodies raised against each AtCYT6 domain were purified from the sera using CNBr-activated Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) with immobilized recombinant AtCYT6-NTD and AtCYT6-CTD according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... LC3 and other proteins were determined from cell extracts by quantitative immunoblots analysis using chemiluminescence detection of horseradish peroxidase-conjugated secondary antibodies on the GE Image-Quant LAS-4010 (GE Healthcare, Waukesha, WI). Image-Quant software (GE ...
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bioRxiv - Cell Biology 2020Quote: ... polymerized from a 1:20 ratio of Cy5-labeled (GE Healthcare) and unlabeled tubulin ...
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bioRxiv - Neuroscience 2021Quote: ... the E domain was subcloned into pGEX-6P-1 (GE healthcare). The GST-gephyrin E domain fusion protein was purified from E ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM EDTA) and loaded onto Superdex S200 column (GE Healthcare). The protein was eluted in buffer C ...
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bioRxiv - Cell Biology 2021Quote: ... and applied to a 1 mL HisTrap HP column (GE Healthcare) using an AKAT Prime Plus (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Cy3-labelled goat-anti-mouse mAb (PA43009, GE Healthcare, 1:3,000) was subsequently used as a secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... and mixed with 125I-Na (1 mCi=37 mBq; GE Healthcare). The reaction was started by adding lactoperoxidase (10μl of 50μg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... A 1 ml HisTrap FF Crude chromatography column (GE Healthcare, USA) was equilibrated with 5 ml of buffer (30 mM sodium phosphate buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1:2000 (Anti-mouse IgG: GE Healthcare Cat#NA931, RRID:AB_772210) and membranes were hybridized 90 minutes at room temperature with agitation on a Nutator (Clay Adams Brand) ...
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bioRxiv - Cancer Biology 2022Quote: ... The PCR product was inserted into pGEX- 5X-1 (GE Healthcare). GST fusion proteins were purified as described (39) ...
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bioRxiv - Molecular Biology 2021Quote: ... equipped with 1-ml HisTrap affinity column (HisTrap HP, GE Healthcare) loaded with Ni (II ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM sodium pyruvate (HyClone, GE Healthcare Life Sciences, catalog # SH3023901), 100 µM β-mercaptoethanol ...
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bioRxiv - Biochemistry 2019Quote: ... and concentrated on a 1 ml HiTrap Q column (GE Healthcare) followed by the isocratic elusion with TEG buffer containing 600 mM KCl (TEG600) ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% (v/v) ampholytes (pH 3-11 NL; GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Aggregated protein (on 1 micron magnetic Sera-Mag beads; GE Healthcare) was then digested with proteomics-grade trypsin (1:75 w/w ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mM DTT) with 10% glycerol using PD10 columns (GE Healthcare), followed by further concentration using an Amicon Ultra 30K device (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... an anion exchange column (HiTrap Q HP 1 ml, GE Healthcare) and another anion exchange column (HiTrap Q FF 5 ml ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 mM DTT) using the AKTA Purifier system (GE Healthcare). The target protein was eluted with 20 ml elution buffer 3 (600 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 mM DTT) using the AKTA Purifier system (GE Healthcare).
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat horseradish peroxidase (GE Healthcare NA935, 1:2,000). Blots were developed by chemiluminescence using Amersham ECL Prime Detection Reagent (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... 1 mM CaCl2 and 10 µg/mL micrococcal nuclease (GE Healthcare) were added and incubated for 12 min at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... 1 mM TCEP) using PD-10 Sephadex desalting column (GE Healthcare). The following samples (50 μL final reaction volume ...
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bioRxiv - Cell Biology 2022Quote: ... or sheep anti-mouse immunoglobulin (GE Healthcare; NA931-1ML; 1:10000) and the enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mL IgG bead suspension (GE Healthcare, cat 17- 0969-01) was washed three times with RP buffer and added to the supernatant ...
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bioRxiv - Genetics 2022Quote: ... and 1 μl Streptavidin Sepharose TM high-performance beads (GE Healthcare) and denatured with a Pyromark Q24 vacuum workstation (Qiagen) ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with anti-mouse IgG (1:10,000; GE Healthcare) for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded in a 1 ml HisTrap (GE Healthcare), equilibrated with 50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 μL Streptavidin sepharose TM high performance beads (GE Healthcare). Next ...