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Citations for GE Life Sciences :
1751 - 1800 of 5577 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... or polyclonal IgG affinity-purified with Protein G Sepharose 4 Fast Flow (GE Healthcare) from heat-inactivated and filtered plasma were mixed with 10 TCID50 of SIVmac239 at a 1:1 ratio (5 μl:5 μl ...
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bioRxiv - Immunology 2023Quote: ... filtered and purified using protein G affinity chromatography following the manufacturer’s protocol (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The protein was passed over a size exclusion column (Superdex 200, GE Life Sciences) in buffer GF (buffer A plus 300 mM NaCl and 1 mM dithiothreitol (DTT)) ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to Protein G Sepharose 4 Fast Flow beads (GE Healthcare) preincubated with or without MLCK mouse monoclonal antibody (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then transferred onto a nitrocellulose blotting membrane (10600003, GE Healthcare Life Science).
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bioRxiv - Neuroscience 2023Quote: Protein from NAc tissue punches was isolated using an Illustra TriplePrep kit (GE Healthcare) according to the manufacturer’s instructions and stored at -80°C until use ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the protein bands were detected using a multicolor fluorescence imaging system (GE Healthcare, USA) after infiltration with Enhanced chemiluminescence (ECL ...
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bioRxiv - Biochemistry 2023Quote: ... HisGST-CK1δ ΔC fusion proteins were purified using Glutathione Sepharose 4B resin (GE Healthcare) using standard approaches and eluted from the resin using 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... the eluted protein was further purified by HiTrap Heparin HP 5ml column (GE Healthcare) and size exclusion chromatography (HiLoadTM16/600 Superdex TM pg ...
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bioRxiv - Cell Biology 2024Quote: ... thawed proteins were purified by gel filtration on an AKTA FPLC system (GE Healthcare) using a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 mL column (GE Healthcare), equilibrated with buffer A (50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were visualized by applying Amersham ECL Western Blotting Detection Reagent (GE Healthcare, RPN2236) to the membranes and imaging with the G:BOX Chemi XX6 (Syngene ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was then applied to a 5 mL Q-trap (GE Healthcare) and eluted with a gradient from 100 mM to 1 M NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted protein was bound to Ni2+ resin (Ni Sepharose 6 Fast Flow, GE Healthcare) in 6M guanidine HCl ...
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bioRxiv - Biochemistry 2023Quote: The protein gel was then transferred onto a membrane (GE Healthcare Life Science UK) and the membrane blocked in 5% semi skimmed milk powder in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins in the supernatant were further purified by HiTrap Heparin HP column (GE Healthcare) with A Buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2023Quote: ... Marker proteins were from the isoelectric focusing calibration kit (high pH range, GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was further purified using a Superose 6 increase column (GE Healthcare) pre-equilibrated with the binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... GST fusion proteins or GST alone were bound on glutathione-Sepharose resin (GE Healthcare) and incubated with 5 μg of purified Snu71 or BBP protein in pull-down buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... GP63 and α-Tubulin was performed by transferring proteins to nitrocellulose membranes (GE healthcare). The membranes were blocked ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane (Amersham Protran 0.45 NC, GE HealthCare) and stained with Ponceau red ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were isolated using Nickel beads (His Mag Sepharose Ni, GE Healthcare) in the QIAGEN BioSprint machine ...
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bioRxiv - Microbiology 2023Quote: ... the filtered protein-nanodisc solution was purified using a Superose 6 column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was purified by anion exchange chromatography on a HiTrapQ column (GE Healthcare) via a linear NaCl gradient and twice by size exclusion chromatography using a Superdex S200 26/60 column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of anti-HA monoclonal antibodies crosslinked to protein G MicroBeads (GE Healthcare) were added to the cleared lysate and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted protein solution was quantified using the 2-D Quant Kit (GE Healthcare) following the manufacturer’s instruction.
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bioRxiv - Microbiology 2024Quote: ... Protein bands reacting with antibodies were visualized using chemiluminescent detection reagent (GE Healthcare®) on an iBright FL1500 (ThermoFisher Scientific®).
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein fractions were run through a desalting column (PD-10, GE Healthcare), the buffer was exchanged into 3 mL ion-exchange Buffer A (50 mM Bis-tris pH 7.0 ...
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bioRxiv - Bioengineering 2024Quote: ... The eluted protein was further purified on a StrepTrap HP affinity column (GE Healthcare) eluted using 10 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein bands were detected using an Amersham Imager 600 UV (GE Healthcare, Eindhoven, Netherlands) and visualized using ImageJ-Fiji software (version 1.53t ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were concentrated to 10 mg/mL using vivaspin 10 MWCO concentrators (GE healthcare). Crystallisation screens were performed by sitting drop vapour diffusion using 3 Lens Crystallisation Plates (SWISSCI ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were applied onto a Superose 12 10/300 GL column (GE Healthcare), which had been pre-equilibrated with a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Bioengineering 2024Quote: ... Refolded proteins were further purified on a Superdex-200 16/600 column (GE Healthcare) using 20 mM TrisHCl [pH 8.0] ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were purified from the supernatant using a His-trap affinity column (GE Healthcare) with a stepwise elution method ...
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bioRxiv - Microbiology 2024Quote: ... The GST fusion proteins were affinity purified via glutathione-sepharose (GE Healthcare, Dornstadt, Germany) by gravity flow ...
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bioRxiv - Genetics 2024Quote: ... Chromatin fragments bound to antibody were captured on Protein A-Sepharose beads (GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were wet transferred overnight onto a 0.45-μm nitrocellulose membrane (GE Healthcare) in ice-cold Towbin buffer at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were then washed 3 times in TBS-T and bands visualized with Amersham ECL chemiluminescence reagent (GE Healthcare). Blots were stripped (62.5 mM Tris-HCl pH 6.5 ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... sensorchip in HBS-EP buffer (10 mM Hepes pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% surfactant P20) (GE Healthcare). All immobilization steps were performed at a flow rate of 2 µl/min with a final concentration of 100 µg/ml.
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
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bioRxiv - Genetics 2020Quote: ... then washed for 3 × 20 min in 1× SSC and 0.1% SDS and imaged using a phosphorimager (GE Healthcare) and scanned using an Amersham Typhoon 5 scanner ...
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bioRxiv - Bioengineering 2020Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Biochemistry 2021Quote: ... flow rate 0.8 ml/min) or Superdex 200 Increase 5/150 (~3 ml, flow rate 0.45 ml/min) columns (GE Healthcare) were used ...
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bioRxiv - Cell Biology 2021Quote: ... SidK1-278-3×FLAG was further purified with a Superdex 200 10/300 Increase gel filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Samples were concentrated L using a 3 kDa Vivaspin™ 20 filter column (GE Healthcare, Mississauga, ON, μ Canada). The conformational mixtures were purified via size exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2021Quote: ... 3 mM CaCl2 and 0.02% DDM) was injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), equilibrated with buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: Full-length mouse Csk (WT, H21I, K43D, and K222R3) were subcloned into a pGEX-6P-3 vector (GE Healthcare) containing an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Microbiology 2021Quote: ... or denaturing 0.8% agarose gel for large RNA separation (0.2 to 3 kb) and transferred onto membrane Hybond N+ (GE Healthcare). RNAs were probed with γ32P 5’ end-labeled oligonucleotide (Table S3 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Samples were first fully submerged in 3 mL of Ficoll-Plaque Plus endotoxin-free water (GE Healthcare, Uppsala, Sweden) in either a 6-well culture plate or 3-mL test tube for 1 hour ...
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bioRxiv - Biochemistry 2019Quote: ... was analysed at a constant temperature of 25°C under continuous flow of HBS-PE buffer (10mM HEPES pH 7.4, 3 mM EDTA, 0.005% (w/v) Surfactant P20 (GE Healthcare) at 30μl/min (sufficient to prevent mass transfer effects ...