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Citations for GE Life Sciences :
1701 - 1750 of 4142 citations for Recombinant Human TGFA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the protein complex is purified by binding to a MonoQ Sepharose column (GE Healthcare) at 90 mM (sample dilution with 25 mM HEPES-KOH ...
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bioRxiv - Cell Biology 2024Quote: ... thawed proteins were purified by gel filtration on an AKTA FPLC system (GE Healthcare) using a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were isolated using Nickel beads (His Mag Sepharose Ni, GE Healthcare) in the QIAGEN BioSprint machine ...
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bioRxiv - Microbiology 2023Quote: ... the filtered protein-nanodisc solution was purified using a Superose 6 column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The total proteins were isolated and then purified using glutathione Sepharose 4B (GE Healthcare), Ni Sepharose (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein complex was further purified over a Ni-charged chelating column (GE Healthcare) and eluted by a linear gradient to 500 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... nChIP’ed chromatin bound to Protein G Sepharose beads (GE Healthcare Cat #17-0618-02) were washed 3X with cold 0.5X PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were transferred onto Amersham™ Hybond® P PVDF membranes (GE Healthcare, #10600023) and detected with the following antibodies ...
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bioRxiv - Microbiology 2024Quote: ... 150 µL protein analysed on a Superdex 200 10/300 GL column (GE Healthcare) integrated in an NGC system via constant flow of SEC buffer with known monomeric proteins serving as calibration standards ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were loaded onto a Superdex 200 Increase 100/300 GL column (GE Healthcare) and the correct fractions were pooled ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants of GST-tagged protein homogenates were loaded onto glutathione SepharoseTM beads (GE healthcare). Prior to the elution ...
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bioRxiv - Biophysics 2024Quote: ... The labelled protein was purified on a Superdex 200 16/300 column (GE Healthcare) to separate it from the unreacted dye ...
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bioRxiv - Biochemistry 2024Quote: ... coli as His-tag proteins and were purified using the IMAC principle (GE healthcare). Following purification ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMC) were enriched by density gradient centrifugation of peripheral blood from healthy human donors through a Ficoll-Paque PLUS (GE Healthcare) gradient ...
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bioRxiv - Biochemistry 2019Quote: Human BRG1 AT-BD (residues 1434–1569) or BD (residues 1454-1569) were cloned into a pGEX6p-1 vector (GE Healthcare) from full length BRG1 cDNA obtained from GE Open Biosytems ...
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bioRxiv - Molecular Biology 2021Quote: ... The full-length human eRF3a was expressed in insect Sf21 cells and purified via affinity chromatography using a HisTrap HP column (GE Healthcare) followed by anion-exchange chromatography on a MonoQ column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... Human PBMCs were isolated from leukopheresis packs obtained from the Central Blood Bank (Pittsburgh, PA) via Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... each well in the plate was further incubated with the HRP-sheep anti-mouse or anti-human secondary antibody (1:5000, GE Healthcare) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Microbiology 2021Quote: ... The primary antigen-antibody complex was detected by incubating the blot with goat anti-rabbit IgG HRP (1:10000, Jackson-ImmunoLab) and sheep anti-human IgG HRP (1:5000, GE Healthcare) in 3% milk in PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... lymphocytes and monocytes were isolated from heparinized human peripheral blood by Ficoll-PaqueTM PLUS (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) gradient centrifugation ...
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bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (Human SOX5, clone ID 30343519; mouse Rarb, clone ID 30608242; mouse Rxrg, clone ID 5707723; all purchased from GE Healthcare) were inserted into pCAGIG vector (Addgene plasmid # 11159) ...
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bioRxiv - Microbiology 2022Quote: ... siRNA (Catalog no. L-013607-00-0005) Human DHX9 (1660) siRNA (Catalog no. L-009950-00-0005) were from Ge Healthcare Dharmacon (Colorado ...
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bioRxiv - Biochemistry 2023Quote: The complementary DNA of human ZIP4 (GenBank access number: BC062625) and human ZIP8 (GenBank access number: BC012125) from Mammalian Gene Collection were purchased from GE Healthcare. The ZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Cell Biology 2023Quote: ... was constructed by inserting the cDNA for human SSH1 (869–1049) into the BamHI and NotI sites of pGEX-4T (GE Healthcare). E ...
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bioRxiv - Cancer Biology 2024Quote: ... were isolated from human buffy coats from the New York Blood Center by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare). Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were purified from buffy coats by two gradient centrifugations: PBMCs were initially isolated with Ficoll-Paque (GE Healthcare, Pittsburgh, PA) and subsequently peripheral monocytes were isolated with Percoll (GE Healthcare ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Immunology 2023Quote: FH was isolated from human plasma using an affinity column made up of a monoclonal antibody against human FH (MRCOX23; MRC Immunochemistry Unit, University of Oxford) coupled to CNBr-activated Sepharose (GE Healthcare, UK) as described previously (21) ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2023Quote: Binding of mAbs to human FcRn was performed by measuring surface plasmon resonance (SPR) using a Biacore T200 instrument (GE Healthcare, UK), as described previously (42).
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from heparinised blood and tissue derived mononuclear cells were isolated from mechanically dissociated and filtered human tissue using Ficoll density gradient centrifugation (Ficoll PaquePlus; GE Healthcare, Amersham) or 33% Percoll (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... The labelled protein was applied to a Thiopropyl Sepharose 6B column (GE Healthcare Life Sciences) to remove double labelled protein ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was then loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare), washed with 5 CV of Heparin Wash Buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The eluted protein was purified by Superose6 Increase 10/30 (GE Life Sciences, Cat. #29091596) gel filtration.
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bioRxiv - Biophysics 2020Quote: ... The protein solution was then loaded onto a Superdex 200 10/300 column (GE Healthcare). The peak fractions were pooled and concentrated (Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were passed over an either HisTrap or GstTrap FF 5□ml column (GE Healthcare), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Diluted proteins were subsequently passed over a HiTrap Heparin HP 5□ml column (GE Healthcare). After washing with binding buffer ...
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bioRxiv - Cell Biology 2019Quote: ... and immune complexes were captured using the Protein G Sepharose (17-0618-01, GE Healthcare). Samples for gel electrophoresis were prepared in Laemmli Sample Buffer (LDS ...
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bioRxiv - Cell Biology 2020Quote: ... overnight and proteins further purified with a HiTrap SP HP cation exchange column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... HRP-labeled proteins were detected with Amersham ECL Western Blotting Detection Reagent (GE Healthcare, UK) or Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Cell Biology 2020Quote: ... along with 5µL of protein ladder (Full-Range RPN800E, GE Healthcare Rainbow Molecular Weight Markers). After transfer ...