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Citations for GE Life Sciences :
1701 - 1750 of 2181 citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... in running buffer (20 mM Tris, pH 8, 150 mM NaCl, 1 mM DTT) supplemented with 0.05% of P20 surfactant (GE Healthcare). Anti-MBP murin monoclonal antibody (Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... and eluted in superdex buffer (20 mM Tris [pH 8.0], 300 mM NaCl, 1 mM TCEP) using the AKTA Explorer FPLC system (GE Healthcare). The protein was concentrated to 7.5 mg/ml.
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bioRxiv - Plant Biology 2021Quote: ... α-P4 or α-P6 before subsequent addition of secondary horseradish peroxidase (HPR)-conjugated antibodies (1:20.000; Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Microbiology 2021Quote: ... the obtained supernatants were filtered through sterile PVDF filters (pore size 0.22 μm) and loaded on 1 ml His-Trap columns (GE Healthcare), pre-equilibrated with ten column volumes of each Millipore water and sodium phosphate buffer A ...
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bioRxiv - Microbiology 2020Quote: ... The clarified lysate was then passed using an ÄKTA Purifier FPLC through a 1 ml HisTrap HP column (GE Healthcare), pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG secondary antibodies (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... with a JA30.50 Ti rotor at 20,000 ×g and applied to a HiTrap SP HP column (GE Healthcare; 1 ml) equilibrated in buffer A (20 mM Na-phosphate buffer (pH 7.0) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation for 1 hour at 38,700 x g and passed over a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 15 column volumes of 20 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml, GE HealthCare). The column was loaded with a filtered solution of 100 mM nickel chloride ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged at 18500 g for 30 minutes and the supernatant filtered through a 0.22 µm PES filter and loaded onto a 1 mL Ni column (GE healthcare) in buffer L with 10 mM immidazole ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM TCEP) and the sample was loaded onto a 5 mL HiTrap Heparin HP affinity column (GE healthcare) pre-equilibrated with 5% buffer B (50 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... the concentration of cAMP in lysates from infected THP-1 cells was determined using the cAMP enzyme immunoassay (GE Healthcare) as previously described (57) ...
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bioRxiv - Biochemistry 2022Quote: ... to 1/10 of the starting volume and loaded on a 5 ml Ni2+ HiTrap IMAC HP column (GE Healthcare). A linear 0-75 mM histidine gradient with elution buffer was used to elute proMbxA ...
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Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by horseradish peroxidase-conjugated goat anti-rabbit (1:10,000) and detection on Super RX X-ray film (Fujifilm) using ECL Plus reagent (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The lysate was centrifuged at 18,500 × g for 30 min and the supernatant filtered through a 0.22 µm PES filter and loaded onto a 1 mL Ni column (GE healthcare) in buffer L with 10 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with either 1 ml of Amersham ECL substrate western blotting detection reagent (GE Healthcare lot# 9622301) or for higher sensitivity ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed three times for five minutes with 0.1% PBS-Tween20 and incubated for 1 hour at room temperature with anti-rabbit HRP secondary antibody (GE healthcare) in blocking solution ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... and the supernatant injected (using 1 ml injection loop) in a Superdex 75 10/300GL gel filtration column (GE Healthcare), previously equilibrated with 50mM Tris pH8 buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were incubated with the appropriate horseradish peroxidase (HRP)-labeled secondary antibody (1:5000 dilution; GE Healthcare UK Ltd, UK). Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo ...
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bioRxiv - Cell Biology 2020Quote: ... and within 1 h after taking out of the incubator on a commercial structured illumination microscope (DeltaVision|OMXv4.0 BLAZE, GE Healthcare). All the images were acquired using 488 nm laser with power set to either 10% or 31.5% ...
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bioRxiv - Neuroscience 2021Quote: ... free-floating sections were washed in PBS and incubated overnight in Cy3-conjugated streptavidin (1:1000 dilution; PA43001, GE Healthcare) in “Triton-PBS” (PBS containing 0.3% v/v Triton X-100 [Sigma]) ...
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bioRxiv - Immunology 2020Quote: ... IgGs were purified from ∼1-3 ml of serum using equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted off the resin with 0.1 M glycine pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was centrifuged for 10 minutes at 25,000g and the lysate supernatant was incubated for 3 hours with 1 mL of glutathione sepharose beads (GE Healthcare) pre-washed in lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: PeSkC and RoSBC samples were applied to a 1.8×13cm Sephadex G100 column at a flow rate of 1ml min-1 using an ÄKTAprime plus unit (GE Healthcare). Void volume was calculated by running blue dextran ...
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bioRxiv - Molecular Biology 2022Quote: ... were used at 1:10,000 and blots developed with ChemiGlow detection reagent (ProteinSimple) and visualized using ImageQuant LAS 4000 chemiluminescent camera (GE Healthcare). For RNA-Immunoprecipitations FLAG-Immunoprecipitation from DHX34-GFP-FLAG A5 cells were performed with in NET2 buffer (50 mM Tris-HCl pH7.5 ...
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bioRxiv - Biochemistry 2020Quote: Solid-phase synthesis of oligoribonucleotide was performed on a 1 μmol scale using AKTA Oligopilot plus 10 synthesizer (GE Healthcare) and Custom PrimerSupport™ Ribo A 40 (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... A volume of 50 μl of each sample at 3 mg·ml−1 was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 25 mM Hepes pH 7.8 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were further incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000; GE Healthcare, USA) in PBST-BSA for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation for 1 hour at room temperature proteins were purified using PD MiniTrap™ G-25 columns (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... the myosin was purified by His-tag affinity purification with a 1 ml HisTrap HP nickel-sepharose column (GE Healthcare). The eluted myosin solution was then dialyzed overnight at 4 °C in a low-salt buffer (25 mM imidazole pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Alba proteins were run on an ion-exchange column (HiTrap SP column, 1 ml, GE Healthcare UK Ltd. Buckinghamshire, England) following the manufacturer’s protocol and as described previously (Maruyama et al. ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... The extracellular media was then diluted two-fold with 20 mM HEPES buffer at pH 7.0 and applied to 1 column volume (CV) of DEAE Sepharose Fast Flow Resin (from GE Healthcare) equilibrated with 10 CV of 20 mM HEPES buffer at pH 7.0 (1 CV equaled 2 ml of settled resin for 25 ml cultures and 1 CV equaled 4 ml of settled resin for 250 ml cultures) ...
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bioRxiv - Biophysics 2020Quote: The protein extract was loaded onto a 1 mL HiTRAP Heparin column HP (GE Healthcare Life Sciences, 17-0406-01) equilibrated with Buffer A (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 mL of the clarified lysate was loaded onto a 1 mL HiTrap IMAC Sepharose FF column (GE Healthcare, Illinois) charged with Ni2+ and preequilibrated with buffer A ...
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bioRxiv - Bioengineering 2020Quote: ... and supernatant was filtered with a 0.22 μm filter before loading onto a 1 ml HisTrap HP column (GE Healthcare). Proteins bound to the column were washed with 10 column volumes of washing buffer (50 mM Tris ...
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bioRxiv - Physiology 2021Quote: ... The immunostaining was detected using horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) (GE Healthcare, Buckinghamshire, UK; dilution: 1:5000) or anti-mouse IgG (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... and transferred by western blotting (30V, 1 hour) to polyvinylidene fluoride membrane (P 0.45 μm; 10600023 Amersham Hybond, GE Healthcare). Blots were probed with biotinylated GNA lectin (40 μg/ml ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 1 mg of total cytosolic proteins was injected onto a Superdex® 200 10/300 GL column (GE Healthcare). The SEC elution was performed with the mobile phase [50 mM Hepes/KOH ...
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bioRxiv - Biochemistry 2021Quote: ... and the monomers were isolated in 20 mmol L-1 sodium phosphate pH 8.0 with 0.2 mmol L-1 EDTA by a Superdex 30 column 26/600 (GE Healthcare, UK). The concentration of monomeric Aβ42 was calculated with using an extinction coefficient of 1 424 M-1 cm-1 for (A280−A300) ...
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bioRxiv - Microbiology 2021Quote: ... IgG secondary antibody for 1 h at RT and developed in Amersham ECL Western Blotting Detection Kit according to the manufacturer’s instructions (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
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bioRxiv - Biophysics 2020Quote: ... followed by washing and incubation with the secondary antibody anti-mouse IgG linked to HRP (1:1000, #NA931, GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were soaked with 1:5000-diluted HRP-conjugated anti-rabbit or anti-mouse IgG secondary antibodies (GE Healthcare) in TBST containing 5% skim milk for 30 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... reverse-crosslinked samples were combined and the DNA was purified using solid phase reverse immobilization (SPRI) Sera-Mag SpeedBeads (1.6:1 ratio, GE Healthcare). The final library was eluted with 22 μL ddH2O.
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bioRxiv - Microbiology 2020Quote: ... Clarified cell lysates (following 40 min centrifugation at 11,000 x g and 0.2 μm filtration) were loaded onto prepacked columns (1 ml) of Ni-Sepharose media (His trap, GE Healthcare) using a benchtop peristaltic pump ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A second purification step was performed by size-exclusion chromatography (SEC) with Superdex 75 column (1 x 30) (GE Healthcare) at a flow rate of 0.5 mL/min monitored at 280 nm using a 25 mM Na2HPO4/NaH2PO4 ...