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Citations for GE Life Sciences :
1701 - 1750 of 4008 citations for Japanese Encephalitis Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The tag-removed protein was loaded to a Resource Q anion-exchange column (GE Healthcare) and eluted with a linear NaCl gradient (10 mM to 300 mM) ...
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bioRxiv - Immunology 2020Quote: IgG purification was performed using HiTrap™ Protein G HP (GE Healthcare #17-0404-01) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Protein was column-purified on Nap-5 columns (GE healthcare, Chicago, IL; #17-0853-01) and immunoprecipitated on Sephadex protein A/G beads (Sigma Aldrich ...
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bioRxiv - Pathology 2021Quote: ... and proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (GE Healthcare). After blocking ...
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bioRxiv - Synthetic Biology 2021Quote: ... Eluted protein was further purified on a HiLoadTM 16/600 SuperdexTM 200 column (GE Healthcare) and eluted with Buffer A4 (20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... A second incubation overnight at 4°C with Protein G-magnetic Sepharose beads (GE Healthcare) was used to pull down protein:DNA complexes prior to washing in low salt (120mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were separated on SDS–PAGE and transferred to a Hybond ECL membrane (GE Healthcare) using Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were resolved eletro-trasferred onto polyvinylidene fluoride (PVDF) membranes (GE Healthcare, Chicago, IL, USA). The membranes incubated with the appropriate antibody in Tris buffered with Tween-20 (TTBS) ...
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bioRxiv - Pathology 2020Quote: ... Recombinant proteins were purified with a Ni-NTA resin and Q-Sepharose™ (GE Healthcare). Endotoxin was removed by Pierce High-Capacity Endotoxin Removal Resin (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... SEC was performed by loading the protein onto Superose 6 Increase 10/300GL (GE Healthcare) column with running buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... the proteins were further purified by gel filtration on 200 16/60 columns (GE Healthcare). In 20 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... using multiple runs over a 1 ml Protein-A HiTrap column (GE Healthcare Life Sciences) on an ÄKTA FPLC (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences), equilibrated and eluted in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was applied to a HiLoad Superdex 75 26/600 column (GE Healthcare) equilibrated with SEC Buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... The protein concentration was measured using 2-D Quant Kit (GE Healthcare, Piscataway, NJ, USA).
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bioRxiv - Neuroscience 2020Quote: Protein concentration in sarkosyl extracted samples was determined using the 2D-Quant Kit (GE Healthcare) following the manufacturer’s instructions and 12 µg of protein were loaded into 4-15% gradient gels (Biorad Miniprotean TGX ...
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bioRxiv - Immunology 2021Quote: ... Proteins were transferred by western blot onto PVDF (0.2 µm pore size, Amersham GE Healthcare) using the BioRad wet transfer system (buffer ...
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bioRxiv - Cell Biology 2020Quote: ... protein detection was performed using Amersham ECL Western Blotting detection reagent (GE Healthcare #CA95038-564L). Immunoblot quantifications were performed using ImageJ software (NIH).
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bioRxiv - Immunology 2020Quote: ... the His-tagged proteins were purified using nickel-affinity chromatography (HiTrap chelating, HP; GE Healthcare) with an imidazole gradient (10-250 mM ...
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bioRxiv - Immunology 2021Quote: ... The bound protein was eluted with 100 mM acetic acid on ÄKTA pure (GE healthcare). To remove IgG1 Fc ...
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bioRxiv - Biophysics 2019Quote: ... with the modification that the final protein was purified over a MonoQ column (GE Healthcare) instead of a ResourceQ column ...
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by anion-exchange column and gel filtration column (GE Healthcare). Finally ...
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bioRxiv - Plant Biology 2019Quote: ... proteins were again run in a Superdex 200 increase 10/300 GL column (GE Healthcare) in 20mM citric acid pH 5 ...
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bioRxiv - Biochemistry 2019Quote: ... of pG-ODN was performed using fast protein liquid chromatography (FPLC, ÄKTA Prime, GE Healthcare) with an anion-exchange HiTrap Q HP column (1 mL ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were pre-cleared with 30 μl of protein-G-Sepharose (GE Healthcare Bio-Sciences). Pre-cleared supernatants were incubated with 5 μg of anti-SARS-CoV-2 nucleocapsid antibody mixture (GTX135357 and GTX632269 [6H3] ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred at 70 V for 2 h onto a nitrocellulose membrane (GE Healthcare) using a wet electroblotting system (BIO-RAD) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein detection was performed using Amersham ECL Western Blotting detection reagent (GE Healthcare #CA95038-564L). Immunoblots were quantified using ImageJ software (NIH).
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bioRxiv - Physiology 2021Quote: ... A chemiluminescent system was used to visualize marked proteins (GE Healthcare Life Sciences, Pittsburgh, PA). Images were taken and analyzed with the ChemiDoc imaging system (Bio-rad ...
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bioRxiv - Immunology 2020Quote: ... filtered (0.22 µm) and incubated with protein A coated agarose beads (GE Life Sciences, 17519901) for 2 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was cleaved from the GST tag by incubation with PreScission protease (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... IgG1 antibodies were isolated from cell supernatant using a HiTrap protein A column (GE Healthcare) and Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: SEC analysis of purified N protein was performed by Superdex 200 16/600 (GE healthcare) at 4 °C on an AKTA system ...
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bioRxiv - Neuroscience 2021Quote: ... The protease was subsequently separated from the eluted proteins using a Glutathione Sepharose (GE Healthcare) packed column ...
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bioRxiv - Plant Biology 2020Quote: ... GST-SRK2E and GST-SRK2E K50N proteins were expressed using pGEX6p-2 (GE healthcare, IL) and purified using Glutathione Sepharose 4B resin (GE healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further polished using a Superdex S-200 gel filtration column (GE Healthcare) equilibrated in 200 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further polished using a Superdex S-200 gel filtration column (GE Healthcare) equilibrated in 200 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified using Ni-NTA column and ion exchange chromatography (QFF, GE Healthcare). Thrombin (1:1 000 ...
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bioRxiv - Biochemistry 2021Quote: ... the expressed proteins were first purified by Ni Sepharose 6 Fast Flow beads (GE Healthcare). After removal of the His6-SUMO tag using home-made Ulp1 (SUMO protease) ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were further purified on a HiLoad 16/600 Superdex 200 column (GE Healthcare). For purification of the GST-tagged proteins ...
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bioRxiv - Microbiology 2021Quote: ... Fc-fused nanobody was prepared with prepacked HiTrap® Protein A HP column (GE Healthcare). The produced Fc-fusion protein was analyzed by SDS-PAGE using standard protocols for dimerization ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins was further purified using a 5 mL HiTrap HP Heparin column (GE Healthcare) and eluted with a buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was purified on a Ni2+-nitrilotriacetic acid (Ni-NTA) sepharose resin (GE Healthcare), eluting with a linear gradient of imidazole (100 – 400 mM ...
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bioRxiv - Biochemistry 2020Quote: ... The protein of interest was purified from the medium on HisTrap™ Excel (GE Healthcare) as described above ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant of the soluble proteins was then loaded onto the HisTrap™ Excel (GE Healthcare) column in buffer A and eluted with a linear gradient of 0-100% of 1 M imidazole in buffer A in 20 column volumes (CV) ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent protein mass was loaded on SDS-PAGE and transferred to Hybond ECL (GE HealthCare) nitrocellulose membrane ...
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bioRxiv - Biophysics 2021Quote: ... Standard proteins used were from the gel filtration calibration kit LMW and HMW (GE Healthcare) with the exception of Cryptococcus neoformans acyl-CoA binding protein (ACBP ...
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bioRxiv - Biochemistry 2020Quote: ... Tag-removed proteins were further purified by size-exclusion chromatography (Superdex 75; GE Healthcare Lifesciences). Two functional variants of SR proteins used in our experiments were the RNA binding domain (RBD ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... Protein A Sepharose CL-4B and Glutathione Sepharose 4 Fast Flow were from GE Healthcare Life Sciences (Chicago ...
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bioRxiv - Cell Biology 2020Quote: ... Detection of the proteins was digitally acquired using an Amersham™ Imager 600 (GE Healthcare).
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Fate of the M-phase-assembled centrioles during the cell cycle in the TP53;PCNT;CEP215-deleted cellsbioRxiv - Cell Biology 2020Quote: ... Proteins at gels were transferred to Protran BA85 nitrocellulose membranes (10401196; GE Healthcare Life Sciences). The membranes were blocked with a blocking solution (5% nonfat milk in 0.1% Tween 20 in TBS or 5% bovine serum albumin in 0.1% Tween 20 in TBS ...