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Citations for GE Life Sciences :
1701 - 1750 of 2193 citations for 5 acetyl 2 methylfuran 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and soluble supernatant was purified using HisTrap FF 5 mL immobilized metal ion affinity chromatography columns (GE Healthcare, New Jersey, USA) and eluted with 250 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The buffer salt concentration was diluted to 30 mM NaCl and the solution was flown through a 5 mL HiTrap Q HP (GE Healthcare) and a NiNTA column ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then diluted 10 times with mono Q binding buffer (50 mM HEPES, pH 8.0) and applied to a MonoQ 5/50 GL column (GE Life Sciences) connected to an AKTA Pure system (GE Life Sciences). ...
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bioRxiv - Biophysics 2020Quote: ... The flow-through that contained the protein of interest was then applied to a HiTrap Q 5 mL column (GE Healthcare). The Q column flow-through containing nsp8 or nsp7 was concentrated using a MWCO 3 kDa Amicon Ultra Centrifugal Filter (Merck ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant containing the solubilized proteins was supplemented with 40 μg/ml avidin and subsequently loaded onto a 5-ml StrepTrap HP column (GE Healthcare), which was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... Cleared lysates containing proteins with no His tag were purified by cation exchange chromatography (CIEX) using a 5 ml HiTrap Sepharose SP FF column (GE Healthcare) fitted on an ÄKTA fast protein liquid chromatography (FPLC ...
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bioRxiv - Cell Biology 2020Quote: ... Buffer-exchange into 40 mM Tris-Cl pH 7.0 was carried out using a 5 ml HiTrap™ desalting column (GE Healthcare) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired from 15 fields at 5- or 10-minute intervals using a DeltaVision Elite Deconvolution Microscope (GE Healthcare Biosciences) with a 60x oil immersion objective and a Front Illuminated sCMOS camera ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed with primers 32P-labelled with [alpha-P32]Deoxycytidine 5’-triphosphate (dCTP) using Megaprime DNA labelling system (GE Healthcare) (for primers ...
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bioRxiv - Developmental Biology 2022Quote: ... The media was buffer exchanged with PBS and His-tagged proteins were captured with 5 mL HisTrap Excel columns (GE Healthcare), washed with 20 mM imidazole and eluted with 300 mM imidazole containing PBS buffer ...
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bioRxiv - Microbiology 2022Quote: ... it was passed through a 0.22 µm filter to remove potential precipitates and applied onto a 5 ml HisTrap HP column (GE Healthcare, USA) equilibrated with buffer BZ3 ...
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bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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bioRxiv - Biochemistry 2020Quote: The oligomeric state of proteins and interaction between 14-3-3 and STARD1 (or STARD3) constructs were analyzed by size-exclusion chromatography on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at 0.1 ml/min flow rate using a Varian ProStar 335/ProStar 363 system ...
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bioRxiv - Biochemistry 2020Quote: ... Eluate from GSH affinity chromatography was subjected to heparin affinity chromatography using a HiTrap HP Heparin column (5 mL GE Healthcare) equilibrated with NMR buffer (10 mM MES ...
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bioRxiv - Cancer Biology 2020Quote: Secreted form of the PD-L1 carrying C-terminal 10x His tag was purified using AKTA Start FPLC and Tricorn 5/100 column (both GE Healthcare) filled with HisPur Ni-NTA Resin (Thermo # 88223 ...
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bioRxiv - Biochemistry 2020Quote: ... The elute was concentrated to 5 ml and applied to a gel filtration run on a 16-600 HiLoad Superdex 200 pg column (GE Healthcare), preequilibrated with 20 mM Hepes (pH 7.0) ...
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bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... and the soluble His6-M protein was purified on an Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with 5 μl rabbit polyclonal anti-GM130 serum or 5 μl pre-immune serum for 60 min at 4°C and incubated with protein A-Sepharose (GE Healthcare) for additional 60 min ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation for 1 hour at 60000 x g and then loaded on a 5 mL Histrap FF column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... of the purified hPIV3 L-P complex was determined by analytical size-exclusion chromatography using a Superdex 200 Increase 5/150 GL column (GE healthcare) with buffer B ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Biochemistry 2020Quote: ... After concentration by spin filtration to 4-5 mL pro-rhodesain was loaded onto a HiLoad 16/600 Superdex 75 pg column (GE Healthcare), and purified by SEC (0.5 mL/min ...
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bioRxiv - Microbiology 2020Quote: ... Diafiltrated cell supernatant was purified by immobilized nickel affinity chromatography (5-ml fast flow crude column and ÄKTA fast protein liquid chromatography [FPLC] system; GE Healthcare) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... coli and purified using our previously reported protocols.17 TNKS2 constructs were cloned into pNIC-MBP expression vectors and an additional purification on an MBPTrap HP 5 ml column (GE Healthcare) prior to cleavage with TEV protease was performed.20 Details of the constructs for each PARP enzyme are listed in Table S1.
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bioRxiv - Cell Biology 2022Quote: ... 5 min at 4 °C followed by an anion exchange chromatography loading the supernatant on a HiTrap Q HP 5 ml column (GE Healthcare) and intensive wash step of 20 column volumes with ion exchange buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT in the presence of TEV protease and subsequently purified using a 5 mL HiTrap HP Heparin column (GE Healthcare), eluting with a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: Purified SMO pCT was concentrated to 5 mg/ml and analyzed via SEC-MALS using a Superdex 75 gel chromatography column (GE Healthcare) equilibrated in gel filtration buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... Uncleaved protein and cleaved hexahistidine tag were removed from the eluted mixture with a HisTrap HP 5 mL column (GE Healthcare) in PBS with 25 mM imidazole ...
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bioRxiv - Immunology 2020Quote: ... Samples containing activity were fractionated by anion exchange chromatography on a Mono Q 5/200 GL column (GE Healthcare, Piscataway NJ) using a gradient from 50mM Tris-HCl (pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... The cell debris was pelleted by centrifugation and the supernatant was loaded onto a 5 ml HisTrap IMAC HP Nickel Sepharose column (GE Healthcare), using an ÄKTA Pure chromatography system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were precipitated the supernatant by addition of ammonium sulfate to 67% saturation and dissolved in buffer D and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer D ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 13,000 g for 60 minutes and the filtered supernatant was loaded onto a 5 mL Ni-NTA column (GE Healthcare, USA) equilibrated with binding buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG-eluted motor domains (wild-type + jordan) were bound to a strong anion exchanger (5/50 MonoQ GL; Cytiva) using a Purifier 10 chromatography system (GE Healthcare). The column was washed with MOPS (10) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved MBP tag and TEV protease were removed by anion exchange chromatography using a Mono Q 5/50GL (GE Healthcare) column pre-equilibrated with buffer-B ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol) to the 50 mM NaCl final concentration and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... and fl-SNED1 (4.5 μM) were injected on Superdex 75 Increase 10-300 GL and Superdex 200 Increase 10/300 GL columns (GE Healthcare) respectively at a flow rate of 0.5 ml/min at 20°C ...
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bioRxiv - Biophysics 2021Quote: ... The pooled fractions were dialyzed against buffer A + 100 mM NaCl before loading onto a 5 mL Hitrap Heparin column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... it was passed through a 0.22 µm filter to remove potential precipitates and applied onto a 5 ml HisTrap HP column (GE Healthcare, USA) equilibrated with buffer BZ3 as described above ...
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bioRxiv - Neuroscience 2022Quote: ... Aβ42 peptides in the filtrate were further concentrated to ∼50 μM at 4°C and 4000×g with a 5 000 Da Vivaspin concentrator (GE Healthcare). The Aβ42 peptide concentration was calculated using an extinction coefficient of 1400 M-1cm-1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Undigested protein and TEV protease were removed by a second Ni-NTA step and flowthrough containing OCS was concentrated to 5 ml for subsequent gel filtration (Hiload 16/60 Superdex 200 pg, GE healthcare). Relevant fractions were pooled together ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein purification was performed on HisTrap FF crude 5 ml Ni-based column using ÄKTA Xpress System (Cytiva, former GE Healthcare). For that a wash buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was first purified by Immobilized Metal Affinity Chromatography (IMAC) by loading the clarified lysate onto a 5 mL Nickel column (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates from 8 L of yeast treated cells were then subjected to a 5 ml Nickel affinity purification using an AKTA purifier system (GE Healthcare) equilibrated in the urea buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The mTORC1 complex and free RAPTOR were separated by anion exchange chromatography on a 5 mL HiTrap Q column (GE Healthcare). The fractions containing mTORC1 complex and free Raptor were concentrated to 1.3 mg/mL and 0.5 mg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed and cleared lysate prepared as described and loaded onto a 5 mL Strep-Trap HP column (GE Healthcare), equilibrated with buffer D ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 or 10 μg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...