Labshake search
Citations for GE Life Sciences :
1651 - 1700 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Immunoreactive proteins were identified with HRP-conjugated anti-rabbit IgG (GE Healthcare) and anti-mouse IgG (GE Healthcare) ...
-
bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by Ficoll-Hypaque density gradient centrifugation (GE healthcare). Monocytes were isolated from PBMCs using magnetic CD14+ isolation beads (Miltenyi) ...
-
bioRxiv - Biochemistry 2022Quote: ... The complementary DNA of human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare. The ZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
-
bioRxiv - Cell Biology 2023Quote: ... Fibronectin was isolated from human plasma using Gelatin-Sepharose 4B (Cat No. 17-0956-01, GE Healthcare, Sydney, Australia) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats using Ficoll-Paque PLUS (GE Healthcare Bioscience). The PBMCs were cryopreserved in 40% heat-inactivated fetal bovine serum (FCS ...
-
bioRxiv - Biochemistry 2024Quote: The complementary DNA of human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare. The hZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
-
bioRxiv - Immunology 2024Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from the blood of healthy donors using Ficoll-Paque (GE Healthcare) and CD14+ monocytes were then positively selected using magnetic beads (Miltenyi) ...
-
bioRxiv - Biochemistry 2024Quote: ... Commercial human fibrinogen (America Diagnostica Inc) was desalted on a 5-ml HiTrap desalting column (GE Healthcare, IL, USA), eluted at a constant flow rate (1 ml/min ...
-
bioRxiv - Biophysics 2021Quote: Proteins were purified on a Ni-NTA HiTrap HP column (GE Life Sciences). The purification tag was cleaved by the appropriate protease (see Construct Preparation ...
-
bioRxiv - Biochemistry 2020Quote: ... The antibody ligands were captured on a Protein A chip (GE Life Sciences) and human CD45 D1-D4 (amino acids 194-578 ...
-
bioRxiv - Bioengineering 2021Quote: ... on a Äkta Express fast protein liquid chromatography system (FPLC, GE Healthcare, USA). The lysate was loaded on the column which was afterwards washed with 12 column volumes Ni-wash buffer (50 mM NaH2PO4 ...
-
bioRxiv - Biophysics 2020Quote: ... hTRPC5 protein was further purified by size exclusion chromatography (Superose-6, GE Healthcare) in buffer containing TBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... were coupled 2 h at RT to protein A Sepharose beads (GE Healthcare). Coupled beads were covalently crosslinked using dimethyl pimelimidate according to standard procedures ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare). Chemiluminescence was detected with ChemiDoc Touch Imaging System (Bio-Rad Laboratories) ...
-
bioRxiv - Cell Biology 2020Quote: ... The proteins were visualized by enhanced chemiluminescence using a LAS-4000 (GE Healthcare).
-
bioRxiv - Cell Biology 2020Quote: ... freshly purified GST-RILP protein bound to GSH-Sepharose 4B beads (GE Healthcare) was incubated with extracts from transfected HEK293T cells (one 10-cm diameter dish per assay ...
-
bioRxiv - Microbiology 2020Quote: ... The protein was further purified by a Mono Q 5/50 (GE Healthcare; a linear gradient of 0–1 M NaCl in 20 mM Tris-HCl (pH 8.0 ...
-
bioRxiv - Microbiology 2020Quote: ... The protein was then loaded into a 1-mL HisTrap column (GE Healthcare) that had been equilibrated with buffer A [100 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Developmental Biology 2021Quote: ... and proteins blotted onto a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences). The membranes were then blocked with 5% skimmed milk in Tris-Buffered Saline Tween (TBS-T ...
-
bioRxiv - Developmental Biology 2020Quote: ... and the proteins were then purified by using Ni-Sepharose beads (GE Healthcare) or GST Sepharose beads (GE Healthcare) ...
-
bioRxiv - Cell Biology 2022Quote: ... Antibody-treated samples were next incubated with Protein A-Sepharose beads (GE Healthcare) at 4 °C for 1 hour ...
-
bioRxiv - Neuroscience 2021Quote: ... The proteins were detected using enhanced chemiluminescence (ECL) detection kit (GE HealthCare, RPN2235).
-
bioRxiv - Molecular Biology 2020Quote: ... the proteins were loaded into HiLoad 16/60 Superdex 75 pg (GE Healthcare) in Buffer E (20 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein was affinity purified with streptavidin sepharose high performance beads (GE Healthcare), washed three times with l × lysis buffer containing 200 mM NaCl and 0.1% TritonX-100 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The soluble protein was purified by absorption to Ni2+-NTA resin (GE Healthcare), washed with Ni-buffer A (20 mM HEPES ...
-
bioRxiv - Cell Biology 2022Quote: Cell lysates were incubated with Quickstain Cy5 protein-dye (GE Healthcare Bio-Sciences) for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... The concentrated medium was purified using a HiTrap protein A column (GE Healthcare) as previously described (41 ...
-
bioRxiv - Genomics 2020Quote: ... Proteins were detected and measured using a LAS-4000 image analyzer (GE Healthcare) in SYBR–green fluorescence detection mode ...
-
bioRxiv - Microbiology 2020Quote: ... Protein quantification was performed using ImageQuant TL 8.1 software (GE Healthcare Life Sciences).
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 100 V for 180 min on ice in the transfer buffer without SDS/EDTA ...
-
bioRxiv - Immunology 2021Quote: ... The protein was subsequently applied to a 1 mL StrepTactin column (GE Healthcare) yielding pure αMβ2 headpiece ...
-
bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins were pull-down with glutathione Sepharose 4B beads (GE Healthcare), pre-washed with ice-cold PBS ...
-
bioRxiv - Developmental Biology 2020Quote: ... Proteins were then transferred to nitrocellulose membranes (Amersham; 0.45 µM NC; GE Healthcare) at 360 mA for one 1 h or 30 mA overnight in blotting buffer (10% v/v 10x running buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were visualized on X-Ray films using ECL detection reagents (GE healthcare) with varying exposure time (0.1 – 2 min).
-
bioRxiv - Immunology 2020Quote: ... with either a research grade Series S Sensor Chip Protein G (GE healthcare) or Series S Sensor Chip CM5 (GE healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... The eluted proteins were concentrated and purified by heparin affinity chromatography (GE Healthcare) using buffer A (30 mM HEPES pH 7.8 ...
-
bioRxiv - Immunology 2021Quote: Serum IgG antibodies were purified by protein G column chromatography (GE Healthcare, USA). The purified IgG antibodies were detected by SDS-PAGE ...
-
bioRxiv - Microbiology 2021Quote: ... The protein was then loaded into a 1-mL HisTrap column (GE Healthcare) that had been pre-equilibrated with buffer A [100 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Microbiology 2021Quote: ... The proteins were further purified using S75 or S200 gel filtration (GE Healthcare). The purity of proteins was accessed on SDS-PAGE ...
-
bioRxiv - Biochemistry 2021Quote: ... All other hexa-histidine-tagged proteins were purified using a HisTrap (GE Healthcare) affinity chromatography step followed by thrombin cleavage ...
-
bioRxiv - Biochemistry 2021Quote: ... the SHP2 protein was further purified by HiTrap Heparin HP column (GE Healthcare). SHP2 containing fractions were pooled and finally polished over a Superdex200 10/300 GL size exclusion column (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein samples were collected and purified by a Superdex 200 (GE Healthcare) column17 ...
-
bioRxiv - Neuroscience 2020Quote: ... The protein was purified by batch purification using TALON affinity resin (GE Healthcare) and treated with HRV-3C protease (Novagen ...
-
bioRxiv - Cancer Biology 2021Quote: ... PON1 monoclonal antibody was coupled to protein G-Sepharose 4B beads (GE Healthcare) and eluted as described previously[69] ...
-
bioRxiv - Genomics 2021Quote: ... protein samples were precipitated onto Sera-Mag SpeevBeads (GE Healthcare, #45152105050250 and #65152105050250) using filter plates (Millipore #MSGVN22 ...
-
bioRxiv - Neuroscience 2021Quote: ... The target protein on the membrane was visualized with ECL Select (GE Healthcare) and a Luminescent Image Analyzer LAS-3000 Mini (Fujifilm).
-
bioRxiv - Plant Biology 2021Quote: ... the protein-DNA complexes were analysed with a Typhoon Trio Imager (GE Healthcare) using the 532 nm laser and 580 nm emission filter.
-
bioRxiv - Biochemistry 2021Quote: ... The protein was bound to a 5 ml HiTrap HP column (GE Healthcare), and a linear gradient of increasing NaCl concentration was used to elute the protein fractions ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was desalted on a HiPrep 26/10 desalting column (GE Healthcare) and loaded on an anion-exchange HiTrap Q HP column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... The dimeric ACE2 protein was purified by GammaBind Plus Sepharose beads (GE Healthcare), followed gel filtration chromatography on a Superdex 200 Increase 10/300 GL column ...