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Citations for GE Life Sciences :
1651 - 1700 of 2313 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Biochemistry 2023Quote: ... Insoluble fractions were removed by centrifugation at 20000 g during 30 min at 4°C and the supernatant was loaded on a 1 mL HisTrapTM HP column (GE Healthcare) connected to a NGC chromatography system (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were run at 4 °C overnight as described above and scanned with GE Amersham Typhoon-5 fluorescence gel and blot scanner (GE Healthcare) with red laser.
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... coupled with the epi-fluorescence illuminator Nikon Intensilight C-HGFIE and viral particles were purified with heparin (GE Healthcare; Cat.nr. 170406013). The concentration of the virus was checked with qPCR (fwd ITR primer ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... CpxA31-163-GST was purified at 4°C using a protocol modified from the GST Gene Fusion System Handbook (GE Healthcare). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Labelling reactions proceeded for 40 mins at 4°C and the excess dye was removed using NAP-5 desalting columns (17-0853-01, GE Healthcare). Nanobodies to be used for confocal microscopy were labelled with Alexa488 (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF, GE Healthcare) at pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... the protein debris was removed by centrifugation (30000×g, 20 min at 4 °C) and the supernatants were applied to His-Trap chelating column (GE Healthcare), washed by refolding buffer and eluted with the same buffer containing 30 mM imidazole ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with a linear gradient of increasing NaCl concentration from 300 mM to 1 M in 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... SC and the D1 domain of FcμR was first immobilized on the chip: SC was biotinylated at the C-terminal and immobilized on the SA chip (GE Healthcare), whereas FcμR-D1 was immobilized on the CM5 chip to a level of resonance units similar to pIgR ...
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bioRxiv - Microbiology 2023Quote: ... After 2.5 days the diatom cultures were filtered (1.2 µm pore size, GF/C, Whatman, GE Healthcare, Little Chalfont, United Kingdom). The cells were washed off the filter using 5 mL ice-cold methanol/ethanol/chloroform 1:3:1 (methanol ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were filtered and then pelleted at 300 g for 5 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
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bioRxiv - Biophysics 2024Quote: ... The peak elution fractions were pooled and concentrated to 2 mL using a Amicon Ultra 10K MWCO centrifugal filter at 4 °C and was loaded into a HiLoad Superdex 200 16/60 column (GE healthcare) equilibrated with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... The sample was centrifuged (10 min, 20000g, 4 °C) and applied to Superdex 200 10/300 gel-filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4 °C by injecting sample onto a Superose 6 Increase 10/ 300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Biophysics 2024Quote: All SPR binding experiments were performed on a CM5 chip (Cytiva) at 25 °C using a Biacore T200 Instrument (GE Healthcare). The purified CNBH domains were immobilized on the CM5 chip using a standard amine coupling chemistry in the presence of 10 mM sodium acetate buffer at pH 5.5 as the immobilization buffer (buffer used to directly dissolve ligands) ...
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bioRxiv - Bioengineering 2020Quote: ... using the amine coupling kit (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with an ECL-plus kit (GE Healthcare). The intensity of the bands was analyzed by NIH Image J (http://rsbweb.nih.gov/ij/).
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bioRxiv - Cell Biology 2022Quote: ... Cy5-labelling kit purchased from GE Healthcare. Para-aminoblebbistatin (ax-494682 ...
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bioRxiv - Cell Biology 2022Quote: Cy5-labelling kit purchased from GE Healthcare was used for conjugation of human fibronectin (Corning) ...
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bioRxiv - Microbiology 2021Quote: ... and molecular biology kits from GE Healthcare and Promega ...
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bioRxiv - Immunology 2020Quote: ... and Amine coupling kit (GE Healthcare, USA) were used for immobilization of SARS-CoV-2 Spike S1+S2 ECD-His recombinant protein (Sino Biological ...
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bioRxiv - Molecular Biology 2023Quote: ... A PhytoPure DNA Extraction kit (GE Healthcare) was used to complete the DNA extraction ...
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bioRxiv - Bioengineering 2023Quote: ... using amine coupling kit (GE Healthcare, USA). First ...
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bioRxiv - Molecular Biology 2023Quote: ... The Ni-reagent kit (28995043, GE healthcare) method was used to immobilize CP over the Ni-NTA sensor chip (BR100532 ...
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bioRxiv - Immunology 2019Quote: Human neutrophils were isolated under endotoxin-free conditions from heparinized venous blood after density gradient centrifugation by Ficoll-Paque (GE Healthcare Life Sciences, NJ, USA). The neutrophil-rich layer was collected and residual erythrocytes were removed by lysis with an ACK (Ammonium-Chloride-Potassium ...
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bioRxiv - Neuroscience 2021Quote: ... Control human blood samples were processed to remove the most abundant proteins using albumin and IgG depletion SpinTrap columns (GE Healthcare Cat 28-9480-20) following the manufacturers instructions ...
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bioRxiv - Microbiology 2023Quote: HeLa cells grown to 90% confluency were transfected with ON-TARGETplus human Skp1 SMARTpool siRNA or non-targeting siRNA (GE Healthcare Dharmacon, Inc., Lafayette, CO) at a final concentration appropriate to the vessel size ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences). Method for EMSA was modified from a previously published protocol (30) ...
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bioRxiv - Microbiology 2020Quote: ... Time-resolved imaging was performed with a DeltaVision Personal DV wide field fluorescence microscope equipped with Ultimate Focus™ capabilities and an environmental chamber warmed to 37 °C (Applied Precision). Images were taken at 5 or 10 minute intervals.
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bioRxiv - Immunology 2022Quote: ... and resuspended in 24 mL of RT 70% PercollTM (1.088 g/mL at 22 °C; GE Healthcare Life Sciences 17-0891-01). Aliquots of 8 mL cell/PercollTM suspension were overlayed with 4 mL HBSS and centrifuged at 400 xg for 30 minutes RT ...
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bioRxiv - Genomics 2020Quote: Time-lapse imaging of stable RPE1 cells expressing 53BP1-eGFP and Lifeact-RFP was performed in a 37°C climate chamber attached to a Deltavision microscope system and the SoftWorx 4.9 software (Applied Precision/GE Healthcare) using a 40x 1.3 NA oil-immersion objective ...
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bioRxiv - Biophysics 2020Quote: ... Cells were incubated for 3-5 days at 27 °C and transfection efficiency was monitored regularly by observing YFP expression level using a fluorescence microscope (DeltaVision, Applied Precision, LLC). At >50% transfection efficiency ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were clarified by centrifugation (18,000×g for 60 min at 4°C) and incubated with glutathione Sepharose 4B (GE Healthcare, Uppsala, Sweden), applied to a gravity column ...
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bioRxiv - Genetics 2020Quote: Incorporation of [α-32P]-dCTP was measured by spotting 5 μl aliquots of the reaction mixture (after the indicated time points at 37°C) on Hybond N+ membrane strips (GE Healthcare Lifesciences). The membranes were washed (3 × with 2 × SSC and 1 × with 95% EtOH ...
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bioRxiv - Cell Biology 2020Quote: ... Live cell imaging was performed at 25°C (pre-calibrated chamber) on a Delta Vision Microscope (Applied Precision, GE Healthcare, Issaquah, WA) equipped with Olympus 60×/1.40 Plan-Apo objective lens ...
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bioRxiv - Cell Biology 2020Quote: ... Live cell imaging was performed at 25°C (pre-calibrated chamber) on a Delta Vision Microscope (Applied Precision, GE Healthcare, Issaquah, WA) equipped with Olympus 60×/1.40 Plan-Apo objective lens ...
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bioRxiv - Cell Biology 2019Quote: ... The rest was incubated for 1 h at 4°C with 20 µl Ni Sepharose High Performance beads (GE Healthcare Life Sciences) to isolate the nanobodies ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was cleared by centrifugation at 15’000×g for 1 h at 4°C and loaded on a His GraviTrap column (GE Healthcare Life Sciences), washed with 20 mM imidazole in PBS ...
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bioRxiv - Biophysics 2021Quote: ... The column eluate containing cyt c was further purified through a gel filtration Superdex 75 16/600 column (GE Healthcare, Chicago, IL) phosphate buffer (25 mM sodium phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant was transferred to a 50 mL tube containing 250 µl of Glutathione Sepharose 4B beads (GE Healthcare, 17075601) washed twice beforehand in 50 mL of PBS ...
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bioRxiv - Plant Biology 2021Quote: ... Oligomeric states of OsbZIP47 protein was determined by analytical size exclusion chromatography (SEC) performed at 4°C on a Superdex 200 increase column (GE Healthcare Inc.) pre-equilibrated with buffer (25 mM sodium phosphate (pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: GST-fused bacterial expression plasmid for PAX9 was created by subcloning wild type and mutant (c.336C>G) PAX9 in BamHI and SalI site of pGEX4T1 vector (GE Healthcare, USA). E ...