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Citations for GE Life Sciences :
1651 - 1700 of 2387 citations for Human Breast Cancer Metastasis Suppressor 1 BRMS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM MgCl2 + 10% glycerol) was injected manually into a Superdex 200 Increase 10/300 GL (GE Healthcare) matrix column at a constant flowrate of 0.4ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... the binding affinity of fragments 1 – 4 and SlpB were evaluated with Biacore X100 (GE healthcare, Uppsala, Sweeden) with sensor chip protein G (Cytiva ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... to < 1 mL and injected onto a Superdex™ 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Physiology 2023Quote: ... and subsequently probed with secondary anti-mouse/-rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue was then digested with 1 mL of enzyme-free dissociation agent HyClone™ (GE Healthcare Life Sciences) for 5 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered lysate was applied to a 1-ml His-Trap (all FPLC columns were obtained from GE Healthcare) column using an ÄKTA FPLC ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed solution was then loaded on a 1 ml HisTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
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bioRxiv - Genetics 2024Quote: ... the membranes were incubated with a horseradish peroxidase-conjugated against the appropriate species antibody (1:10,000; GE Healthcare) diluted in the blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-rabbit HRP antibody (50 μL/well of a 1:1000 dilution, GE Healthcare, 1h at RT), with washing steps as above ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed in 1 x TBST and incubated in the appropriate secondary antibodies (anti-Rabbit, GE Healthcare, NA934V ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 3h at room temperature in streptavidin horseradish peroxidase (HRP) conjugate (GE Healthcare, 1:200) diluted in 0.3% Triton X-100 in 0.01 M PBS ...
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bioRxiv - Cell Biology 2024Quote: ... which was probed with a horseradish peroxidase-coupled secondary antibody (donkey-α-rabbit IgG, GE HealthCare, 1:400). Subsequent staining was performed using 3-amino-9-ethylcarbazol ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and successful triplicate reactions were pooled and cleaned using the Illustra ExoProStar PCR and Sequence Reaction Clean-Up Kit (GE Healthcare Life Sciences, Pittsburgh PA, USA). Indexing adaptors were added via PCR (8 cycles ...
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bioRxiv - Plant Biology 2023Quote: ... after which their DNA was amplified by multiple displacement amplification (MDA) (Table S1) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by (13) ...
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bioRxiv - Microbiology 2024Quote: The same mixture of phage DNA as mentioned above was amplified by Multiple Displacement Amplification (MDA) using the Genomephi V3 kit (GE Healthcare Life Science, Marlborough, MA, USA) with 10 ng input and incubated at 30°C for 0.5 h (MDA_0.5h ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was collected and incubated for 5 min on 1 mL Ni Sepharose® 6Fast Flow (GE Healthcare) equilibrated with 10 mL binding buffer ...
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bioRxiv - Biophysics 2021Quote: ... 10 μM ZnCl2 and 1 mM DTT) and loaded into a HiTrap Heparin HP 5 ml column (GE Healthcare) equilibrated with 6.25% of buffer HepB (buffer HepA supplemented with 2 M NaCl) ...
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bioRxiv - Biophysics 2019Quote: ... 50 mM KAc,1 mM DTT) Further purification was performed using size exclusion chromatography (Superose-6 increase, GE Healthcare) using Shimadzu High Performance Liquid Chromatograph ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM TCEP] by size-exclusion chromatography using a HiLoad 16/60 Superdex 200 gel-filtration column (GE Healthcare). PelXC232S eluted as a single Gaussian shaped peak ...
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bioRxiv - Cell Biology 2020Quote: ... In a 48 well plate, 25-32.5μL siRNA (for sequences, see table 1) was mixed with 25uL 1x siRNA buffer (GE Healthcare) containing 0.5uL Dharmafect 1 transfection reagent ...
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bioRxiv - Developmental Biology 2021Quote: ... For monoclonal antibody production, the DNA encoding mouse KCTD19 (residue 654-793 aa, NP_808459.1) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and fractions contacting samples were loaded onto size-exclusion column (Superdex 75 16/60, GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... Three washes were performed with TBST followed by 1 h incubation with secondary antibody (ECL, HRP linked, GE Healthcare) and another series of washes ...
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bioRxiv - Microbiology 2019Quote: ... membranes were incubated for 1 hour with secondary α-rabbit antibodies conjugated with horseradish peroxidase (HRP) (GE healthcare, NA934) diluted by 1:10,000 in 5% skim milk in TBST ...
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was loaded at 1 ml/min on a His-Trap FF-crude 5 mL column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were washed with TBS-T and incubated with HRP secondary antibodies (GE Healthcare Life Sciences, 1:2000), and immunobands were detected with a Pierce ECL Western Blotting Substrate (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used were horseradish peroxidase (HRP)-conjugated anti-mouse antibody (sheep polyclonal, used at 1:3000, GE Healthcare), HRP-conjugated anti-rabbit antibody (sheep polyclonal ...
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bioRxiv - Genetics 2020Quote: ... Eluted fractions were diluted 6.5-fold with R buffer and loaded onto a 1 mL ResourceQ column (GE Healthcare). Rrp1 eluted at around 300 mM NaCl with a linear gradient of 0.1-1.0 M NaCl in R buffer ...
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bioRxiv - Genetics 2020Quote: ... then washed for 3 × 20 min in 1× SSC and 0.1% SDS and imaged using a phosphorimager (GE Healthcare) and scanned using an Amersham Typhoon 5 scanner ...
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bioRxiv - Neuroscience 2020Quote: ... before they were incubated with HRP-conjugated secondary antibodies (1:10000, NXA931 and NA934, GE Healthcare Europe, Freiburg, DE) also diluted in blocking solution for 1 h at RT ...
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bioRxiv - Synthetic Biology 2021Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with two different 32P-radiolabeled probes (Church and Gilbert ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using species-appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; GE Healthcare, Cleveland, OH, USA). Western blots were imaged via HRP chemiluminescent detection (Azure Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were centrifuged and the supernatants used for the purification on HisTrap HP columns (1 mL; GE Healthcare) connected to an AKTA Explorer system (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mM DTT and loaded directly onto a 5 ml HiTrap SP Sepharose fast flow column (GE Healthcare). Bound Nup84-Nup133 was eluted from the column using a 15 column volume (CV ...
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bioRxiv - Biochemistry 2020Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Biochemistry 2020Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM Tris pH 7.2 ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... An enhanced chemiluminescence (ECL)-horse radish peroxidase (HRP) conjugated secondary antibody (NA934V, 1:1000 dilution, GE Healthcare, Uppsala, Sweden) and SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... cells were harvested and rAAVs were purified using 1 mL HiTrap heparin columns (GE Healthcare Bio-Sciences, Uppsala, SwedenSigma) and concentrated using Amicon Ultra centrifugal filter devices (Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL, GE Healthcare, Chicago, IL). Polyclonal affinity purified NS3 and NS5 proteins were produced as previously described (20) ...
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bioRxiv - Immunology 2021Quote: ... IgGs were purified from ~1 ml of plasma with equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted from the resin with 0.1 M glycine at pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant proteins were then purified using a 1 ml HisTrap HP column and ÄKTA™ system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting soluble fraction was applied on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) and purified according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... MERS-CoV NTD and SARS-CoV-1 NTD were then further purified using a Superdex 75 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Chromosomal DNA fragments were separated on a 1% agarose gel and blotted onto Hybond N+ nylon membranes (GE Healthcare), following standard techniques (Sambrook and Russell ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...