Labshake search
Citations for GE Life Sciences :
1651 - 1682 of 1682 citations for 6 Methyl 4 trifluoromethyl nicotinonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... on an Akta Pure chromatography system using a Tricorn 10/300 column with Sepharose 4 Fast Flow resin (all GE Healthcare Life Sciences). EVs were then sterilized by 0.45 μm syringe-filtration ...
-
bioRxiv - Biochemistry 2024Quote: ... CezanneCAT was eluted by cleavage of the GST tag with 15 units/ml TEV protease (SigmaT4455-1KU) in PBS buffer overnight at 4°C and desalted using a PD-10 column (GE Healthcare, cat. # 17085101) in 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2019Quote: ... using a HiTrap NHS-Activated HP affinity column coupled with aIL-4 monoclonal antibodies and an ÄKTA Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare, Piscataway, NJ, USA). Immunizations ...
-
bioRxiv - Biochemistry 2020Quote: ... lysates were incubated overnight at 4°C with 400 μl of Glutathione Sepharose 4B per l of expression culture (GE Healthcare GE17-0756-01) with gentle rotation ...
-
bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Western blots were visualized and quantified with enhanced chemiluminescence and imaging on a Fuji LAS 4 000 image station (GE Healthcare, Chicago, IL, US)4.
-
bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad, Hercules, CA) for electrophoresis and transferred to a PVDF membrane (GE Healthcare Amersham, Chicago, IL). For cryba4 (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
-
bioRxiv - Genomics 2021Quote: ... #S0282).[1] Reverse transcription was performed in 1.5 mL microfuge tubes under end-over-end rotation using a modified Reverse Transcription mix (1x Maxima H-RT buffer, 4% Ficoll PM-400 (GE Healthcare, #17-0300-05), 3 mM MgCl2 (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
-
bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
-
bioRxiv - Bioengineering 2020Quote: ... depending on machine availability and the type of imaging performed: LOGIQ Book XP with 8L-RS 4-12 MHz linear array probe (GE Healthcare, Milwaukee, WI, USA), Aixplorer with L14-5 probe (Supersonic Imagine ...
-
DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
-
bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
-
bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
-
bioRxiv - Bioengineering 2019Quote: ... cells debris were removed by centrifugation at 16,000 × g for 20 min at 4 °C and the supernatant (supplemented with 20 mM Imidazole) was loaded on a 1 mL Ni+-HisTrapHP column (GE Healthcare #71-5027-68 AK) and washed with a buffer containing 100 mM Tris-HCl (pH 7) ...
-
bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
-
bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution in 3% milk overnight at 4°C before incubation with secondary HRP-linked sheep anti mouse (NA931-1ML, GE Healthcare Life Sciences, Buckinghamshire, UK) at a 1:3000 dilution in 5% milk for 1 hour at room temperature ...
-
bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
-
bioRxiv - Biochemistry 2021Quote: ... Secreted protein was captured by two round of batch absorption (BA) with 4 mL/L of pre-equilibrated Ni Sepharose beads (GE Healthcare, Cat #17-5318-01). The bound beads were transferred to gravity flow column and sequentially washed with 30 CVs Wash Buffer (50 mM HEPES 7.5 ...
-
bioRxiv - Plant Biology 2022Quote: ... chromosomal DNA was purified and amplified to 5.4 - 7.9 μg by multiple displacement amplification (Supplementary Table 3) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by Šimková et al ...
-
bioRxiv - Cell Biology 2023Quote: ... pombe lysate were mixed and incubated at 4 °C for 30 min before the addition of glutathione-sepharose beads (GE Healthcare, Cat#17-0756-01). After 2-hour incubation ...
-
bioRxiv - Immunology 2023Quote: ... Eluate was then cleaved with TEV protease overnight at 4 °C and subsequently purified further by size exclusion chromatography (Superdex 200 26/600 from GE Healthcare; cat # GE28-9893-36) in TBS ...
-
bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
-
bioRxiv - Cancer Biology 2019Quote: ... The immune complexes were mixed with 20 μl of Protein A or G sepharose 4 Fast flow beads (50% v/v, GE Healthcare Life Sciences, Marlborough, MA, USA). Whole cell lysates and immunoprecipitates were detected by Western blotting ...
-
bioRxiv - Physiology 2019Quote: ... mice were injected with 2mg/kg body weight three times during 4-week period with miR-181a mimic (GE Healthcare, C-310435-05 conjugated to cholesterol) or custom antagomiR-181a (5’-FITC-mA*mC*mUmCmAmCmCmGmAmCmAmGmCmGmUmUmGmAmA*mT*mG*mU*mU - 3’Cholesterol ...