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Citations for GE Life Sciences :
1601 - 1650 of 1839 citations for Upstream stimulatory factor 2 USF2 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatant using a HiTrap protein A column (Cytiva, GE Healthcare). IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva ...
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bioRxiv - Developmental Biology 2020Quote: ... Flag primary antibody (monoclonal M2 mouse IgG1) and enhanced chemiluminescence assay (Amersham ECL Primer, GE Healthcare Life Science) were used for detection.
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bioRxiv - Microbiology 2021Quote: ... The apparent binding affinity (KD) for individual antibody was calculated using BIAcore® 3000 Evaluation Software (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... Signals were visualized with chemiluminescence (34095, Pierce) with a secondary antibody conjugated to Horseradish peroxidase (NXA931V, GE Healthcare). The chemiluminescence signal was obtained by the FUJI Medical X-ray film (4741019289 ...
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bioRxiv - Microbiology 2023Quote: ... membranes were probed with an anti-rabbit or an anti-mouse horseradish peroxidase-linked secondary antibody (GE healthcare) for 45 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare). Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... detection of antibodies was detected using the Amersham ECL Western Blotting Prime Blocking and Blotting Reagents (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... followed by anti-rabbit IgG horseradish peroxidase-linked secondary antibodies (GE Healthcare Life Sciences, UK Ltd., 1:10,000) and Pierce ECL Plus Western Blotting Substrate (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... were used in combination with secondary anti-mouse antibodies conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). For visualization ...
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bioRxiv - Immunology 2023Quote: ... Antibody purification from cell supernatants was done in two steps using the ÄKTA Avant purification system (GE Healthcare). The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe ...
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bioRxiv - Genetics 2024Quote: ... the membranes were incubated with a horseradish peroxidase-conjugated against the appropriate species antibody (1:10,000; GE Healthcare) diluted in the blocking buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed in 1 x TBST and incubated in the appropriate secondary antibodies (anti-Rabbit, GE Healthcare, NA934V ...
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bioRxiv - Molecular Biology 2024Quote: ... The following secondary antibodies were used: anti-mouse IgG (NA0310V) and anti-rabbit IgG (NA9340V) from GE Healthcare for immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: ... which was probed with a horseradish peroxidase-coupled secondary antibody (donkey-α-rabbit IgG, GE HealthCare, 1:400). Subsequent staining was performed using 3-amino-9-ethylcarbazol ...
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bioRxiv - Bioengineering 2019Quote: ... 2 mL of human buffy coat/fresh blood mixed well with 2mL of phosphate-buffed saline (PBS) and then carefully layered on top of 2 mL of Ficoll (GE Healthcare Life Sciences, US) inside a 10ml centrifuge tube without mixing ...
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bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated in Buffer B ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein were separated on 4-15 % Tris-glycine (TG) gels (#45101080003-2, SMOBIO, Hsinchu, Taiwan) and electro-transferred onto polyvinylidene fluoride (PVDF) membranes (#A16646282, GE Healthcare, Chicago, IL, USA) in TG buffer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... was passed through the HisTrap column previously equilibrated with IMAC buffer A at 2 ml/min flowrate with a peristaltic pump (GE Healthcare Pump P-1) and then washed with 5 CVs of buffer IMAC A supplemented with 50 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... were mixed together at a molar ratio of 1:2 and loaded on a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare, Chicago, IL, USA) pre-equilibrated with 20mM Tris-HCl (8.0) ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following the manufacturer’s protocol using 15 µl of PCR product and 2 µl Streptavidin Sepharose High Performance beads (GE Healthcare, cat. GE17-5113-01).
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bioRxiv - Molecular Biology 2023Quote: ... 300 μM 5’ thiol-modified 18-nt DNA oligonucleotides—comprised of an AAA base sequence and a 15-base secondary probe binding sequence (Eurofins)—were reduced with 50 mM dithiothreitol in PBS at room temperature for 2 hours and purified using NAP5 columns (GE Healthcare 17-0853-01). Maleimide-activated antibodies were then combined with 11 equivalents of the reduced form of the thiol-modified DNA oligonucleotides in PBS and incubated at 4℃ overnight ...
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bioRxiv - Microbiology 2020Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with corresponding antibody and chemiluminescence was visualized according to the manufacturer’s instructions (ECL Advanced; GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... The following antibodies were used: anti-GFP (7.1/13.1, mouse monoclonal IgG, secondary antibody peroxidase conjugated sheep anti-mouse IgG, NA931V, GE healthcare) or anti-Flag (M5 ...
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bioRxiv - Developmental Biology 2021Quote: ... For monoclonal antibody production, the DNA encoding mouse KCTD19 (residue 654-793 aa, NP_808459.1) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Immunology 2021Quote: ... or Amersham ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody (NA931, GE Healthcare UK Limited, UK) in the blocking buffer for 3 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-chromatin complexes were purified with Protein A conjugated to sepharose beads (GE Healthcare, 30 µL GE17-5280-01) for 2 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified using MabSelect Protein A followed by Capto Q according to manufacturer protocols (GE Healthcare, Chicago, IL). Purified antibodies were concentrated and formulated to ≥25 mg/mL in PBS.
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibody conjugated with horseradish peroxidase was detected with ECL plus chemiluminescence kit (Amersham Biosciences/GE Healthcare, Piscataway NJ). The images were acquired with an HP Scanjet and densitometry determined using NIH ImageJ ...
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bioRxiv - Biochemistry 2021Quote: GST-MLLE protein was applied to Series S sensor chip CM5 bound with anti-GST antibodies (GE Healthcare BR100223), in Biacore T100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were detected against mouse or rabbit IgGs and visualized with ECL Western blot detection solution (GE Healthcare) or Odyssey infrared imaging system (LI-COR ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with horseradish peroxidase-conjugated sheep anti-mouse IgG polyclonal antibody (GE Healthcare, Chicago, IL, USA). Antigen-antibody interactions were detected using o-phenylenediamine solution as the substrate (Nacalai Tesque) ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated with a rabbit polyclonal anti-HDA15 antibody and protein G Mag Sepharose beads (GE Healthcare) overnight at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein-antibody complexes were detected on X-ray films following addition of Enhanced Chemiluminescence (ECL) substrate (GE Healthcare, UK). The relative density of the pCREB and CREB bands was quantitated using ImageJ software (NIH ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies (GE Healthcare Life Sciences, Marlborough, MA).
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bioRxiv - Microbiology 2022Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
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bioRxiv - Microbiology 2022Quote: ... IgG1 and IgG2 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibody staining using either sheep α-mouse or donkey α-rabbit (NA931V, NA934V, GE HealthCare, Chicago, IL, USA) was performed for 1 hour on a tube roller at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The antibodies were purified on an AKTA FPLC using GE Superdex 200 increase 10/300 GL column (GE HealthCare) in 1× PBS.
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bioRxiv - Microbiology 2022Quote: ... antibodies diluted 1:500 in 1 U/mL DNAse I in Tris Buffered Saline containing 1% BSA (GE Healthcare). Three 5-minute washes with block (1% BSA with 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was incubated with secondary antibodies and visualized with the ECL Detection System (GE Healthcare, Little Chalfont, UK).
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... a CM5 sensor chip was covalently modified by an anti-GST polyclonal goat antibody (GST capture Kit, GE Healthcare). The surface of flow cell 1 and 2 was activated for 60 s with 50 mM NaOH (10 μL/min) ...