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Citations for GE Life Sciences :
1601 - 1650 of 4374 citations for N 2 Chloro 5 1 oxo 2 3 pentadecylphenoxy butyl amino phenyl 4 4 dimethyl 3 oxovaleramide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... see SI Table 5): Clarified lysate was applied to a 5 mL HisTrap FF column (GE Healthcare) equilibrated with His-binding buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... The reduced 5′ thiol-modified ssDNA was purified into water using NAP-5 columns (GE Life Sciences). The reduced 5’ thiol-modified ssDNA was spin vacuum dried ...
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bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR at 10 mg mL-1 (340 μM) was injected (70 μL) onto an inline Superdex S200 5/150 Increase (GE Healthcare), equilibrated with buffer C ...
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bioRxiv - Molecular Biology 2020Quote: Wild type or ΔN20 hMcm10-ID (200 µL at 5 mg ml-1) was injected into Superdex 75 Increase 10/300 GL column (GE Healthcare) operating with the elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved KIX was purified using a linear gradient of 50 mM to 1 M NaCl over ten column volumes by cation exchange chromatography using MonoS 5/50 GL column at a flow rate of 1 mL per minute (GE Healthcare). Fractions containing purified KIX were confirmed by SDS-PAGE and dialyzed against 1 L of 50 mM potassium phosphate pH 5.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was next loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was then loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation for 1 hour at 60000 x g and then loaded on a 5 mL Histrap FF column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... 70 μL of each sample at 1 mg/mL was fractionated in GF150 buffer using a Superdex 200 5/150 analytical gel filtration column (GE Healthcare) run at 0.1 mL/min on an Agilent 1200 series LC system collecting UV at 280 nm before then passing through the light scattering and refractive index detectors in a standard SEC MALS format ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... was applied to a previously equilibrated HiTrap Chelating HP 5 ml column charged with 50 mM CoCl2 and coupled to a GSTrap HP 1 ml column (both GE Healthcare). Buffer A was used for equilibration ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction products were dot-blotted and UV cross-linked onto a Hybond N nylon membrane (GE Healthcare) followed by hybridized at 50 °C with end-labeled (CCCTAA)4 to detect C-circles or (TTAGGG)4 to detect G-circles.
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bioRxiv - Molecular Biology 2021Quote: ... The eluted DNA was dot-blotted and UV cross-linked onto a Hybond N nylon membrane (GE Healthcare) and hybridized with a telomere (or a tubulin ...
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bioRxiv - Immunology 2021Quote: ... each ectodomain variant was chemically cross-linked to N-hydroxysuccinimide (NHS)-activated Sepharose beads (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: RNA was separated on a 16% polyacrylamide gel and transferred to the Amersham Hybond-N+ membrane (GE Healthcare). Probes tRNA-Glu-TTC ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA gel was electroblotted onto an Amersham Hybond-N neutral membrane (GE Healthcare UK Limited, Buckinghamshire, UK) at 180 mA for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... Sera-Mag Oligo(dT)-coated magnetic particles and Hybond-N+ membranes were obtained from GE Healthcare (Chicago, IL). GENEzol™ reagent was purchased from Geneaid Biotech (New Taipei ...
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bioRxiv - Immunology 2021Quote: ... mRNA was mixed with RNA loading buffer and then carefully spotted onto a Hybond-N+ membrane (GE Healthcare), followed by UV crosslinking ...
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bioRxiv - Molecular Biology 2023Quote: ... resolved on a 0.8% agarose gel and transferred onto a Hybond-N positively charged membrane (GE Healthcare, UK). Oligonucleotide primers were used to amplify a Maxi-probe (SMD359/6 ...
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bioRxiv - Microbiology 2022Quote: ... Separated DNA fragments were transferred to the nitrocellulose membranes (Amersham Hybond-N+; GE Healthcare UK Ltd., Buckinghamshire, UK) by capillary transfer ...
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bioRxiv - Microbiology 2022Quote: ... 0.3 M sodium citrate pH 7.0) and transferred to an Amersham Hybond-N+ membrane (GE Healthcare Life Sciences) by capillary action overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length Nedd4L (N-terminal GST-tagged, C-terminal His6-tagged) was purified by glutathione– Sepharose (GE Healthcare) column chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare). Recombinant His-tagged PHB1 was incubated with genistein beads (0.5 mg ...
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bioRxiv - Molecular Biology 2023Quote: ... twenty-one-residue-long peptides based on MITF residues 314 to 333 were synthesized on amino-functionalized cellulose membranes (Whatman Chromatography paper Grade 1CHR, GE Healthcare Life Sciences #3001-878) and the presence of SPOTed peptides was confirmed by ultraviolet light (UV ...
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bioRxiv - Molecular Biology 2021Quote: ... the immobilized proteins were eluted and further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were assessed by SDS–PAGE followed by Coomassie blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted out and further purified by a mono Q 5/5 column (GE Healthcare) and a Superose S6 Increase 5/150 GL column (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... 5% newborn calf serum (GE Life Sciences), and 1x penicillin/streptomycin in an atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... HisTRAPTM TALON column (5 mL, GE healthcare) was connected to an AKTA prime chromatography system (GE healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... and MonoQ 5/50 GL (GE Healthcare). HsMSH2-MSH6 containing fractions were dialyzed in storage buffer (25 mM HEPES pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: ... (5) Source 15 RPC resin (GE Healthcare) chromatography using a linear gradient from 0 % to 86 % (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... a 5 ml GSH column (GE Healthcare) was mounted after the size-exclusion column ...
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bioRxiv - Biochemistry 2021Quote: ... a 5 ml Talon column (GE Healthcare) was mounted after the size-exclusion column ...
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bioRxiv - Immunology 2021Quote: ... and 5 mL affinity columns (GE Healthcare) on an AktaPure chromatography system ...
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bioRxiv - Biochemistry 2022Quote: ... Mono Q 5/50 GL (GE Healthcare); L-α-phosphatidyl-L-serine (Sigma Aldrich) ...
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bioRxiv - Physiology 2022Quote: ... supplemented with 5% serum (Hyclone, GE Healthcare), 1% penicillin-streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% Hyclone FBS (GE Healthcare), 0.5 ng/ml 17 β-oestradiol ...
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bioRxiv - Biochemistry 2022Quote: ... three 5 mL StrepTrap HP (GE Healthcare) were connected to increase the amount of isolated protein per flow through ...
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bioRxiv - Biochemistry 2022Quote: ... and HiTrap Heparin (5 ml, GE Healthcare) columns that had been equilibrated in tandem with QA buffer ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM of each NTP (GE Healthcare), 0.01% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: ... 5 mL column (GE Healthcare Life Sciences), which was first washed with 100 mL Wash buffer (50 mM Tris ...