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Citations for GE Life Sciences :
1601 - 1650 of 3218 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... ∼1 mg of protein was desalted into 100 mM sodium bicarbonate (NaHCO3) at pH 8.3 using a PD10 desalting column (GE Healthcare 17085101) according to manufacturer’s suggestions ...
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bioRxiv - Biophysics 2022Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer plus 0.03% digitonin ...
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bioRxiv - Biochemistry 2022Quote: SEC experiments were performed on 100 μL injections of 70 μM ScMlp11-325 with a Superdex 200 (10/300) GL column (GE Healthcare) at 0.5 mL min−1 at 25°C in 500 mM NaCl ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Each strain was subsequently transferred to a 100 mm Petri dish with an overlay of sterile autoclaved Hybond nylon membranes (84 mm) obtained from Amersham (GE Healthcare). The strains were then allowed to grow for 2-3 weeks on PDA media overlaid with this membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... The purified MUS81-EME1 was subjected to cation-exchange chromatography (CEC) with a Mono S 4.6/100 PE column (GE Life Sciences) on ÄKTA explorer FPLC system ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to ∼2 µM for cryo-EM sample preparation.
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bioRxiv - Molecular Biology 2024Quote: ... the eluate from the Ni-column was diluted to 100 mM NaCl and further purified using a Q-column (GE Healthcare). Bound proteins were eluted by a linear gradient to 1 M NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... A 3.5-meter column with BC2L-A agarose beads was used for chromatographic separation at 100 µL/min using Äkta purifier (GE Healthcare) at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3C protease was removed by Ni-chelating sepharose and the heterotrimetric G-protein was further purified with MonoQ 10/100 GL column (GE Healthcare). Protein was bound to the column and washed in buffer A (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The purified receptor was subsequently concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and run on size-exclusion chromatography on a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated with 30 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 2µM for cryo-EM sample preparation.
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bioRxiv - Biophysics 2024Quote: ... The fractions containing the protein were collected and concentrated using 100 kDa cutoff concentrator and loaded onto Superdex 200 10/300 GL column (GE Healthcare) using 20 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with blocking solution (2.5 % FBS, 200 mM glycine, 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...
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bioRxiv - Biophysics 2023Quote: ... developed with a salt gradient of 100 mM – 600 mM NaCl over 75ml followed by gel filtration on a Superdex 200 10/300 column (GE Healthcare) in buffer 20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... To remove any residual impurities final step of size-exclusion chromatography on a Hi load 16/60 Sephacryl S-100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 was employed ...
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bioRxiv - Molecular Biology 2023Quote: ... Column was washed with binding buffer and the proteins of interest were eluted with the binding buffer supplemented with 250 mM imidazole and applied to size exclusion chromatography column Hi load 16/60 Sephacryl S-100 column (GE Healthcare). Fractions of interest were pooled ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer with 1 mM MgCl2 and 0.03% digitonin ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer with 0.03% digitonin ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated with a 100 kDa cutoff concentrator to 500 μL and loaded on a Superose 6 Increase 10/300 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: To create BAC transgenic mice 10 μg maxi-prepped BAC DNA (Nucleobond 100) was linearised by restriction digest (NotI) and purified by sepharose column purification (GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were then resuspended in 650 µL of dialysis buffer and 100 U of Thrombin (GE Healthcare, #GE27-0846-01), to a final volume of 1 mL ...
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bioRxiv - Developmental Biology 2023Quote: ... Each batch of imbibed seeds was equally distributed on 6 ½ MS plates containing 0.8 % plant agar and 0.5% sucrose (ca. 100 µl seeds per plate) topped with filter paper (Whatman Shark Skin Filter Paper, GE Healthcare #10347509). Seedlings were grown on the filter paper-topped MS agar plates in a growth chamber under long-day conditions ...
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bioRxiv - Biophysics 2023Quote: ... The eluted sample was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and purified by Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Microbiology 2024Quote: ... The fractions containing the purified SpyCatcher003-mi3 were pooled and concentrated using a Vivaspin 20 100 kDa molecular weight cut-off centrifugal concentrator (GE Healthcare).
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bioRxiv - Plant Biology 2024Quote: ... The enriched nuclei were further purified using three-step Percoll-gradients (100%/60%/30%, 17-0891-01, GE Healthcare, Solingen, Germany) modified after Marienfeld et al ...
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bioRxiv - Biophysics 2021Quote: ... and the supernatant was loaded onto a 5 ml HisTrap HP column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... in 0.5 mL fractions using a pre-equilibrated PD-10 desalting column (GE Healthcare) and concentration was measured on a NanoDrop using a theoretical extinction coefficient of 10095 M-1cm-1 calculated from the amino acid sequences (all FapC variants contain 1 Trp ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was then loaded onto 1 mL HiTrap Heparin HP column (GE Healthcare) and washed with 10 CVs Heparin Buffer (50 mM NaHEPES ...
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bioRxiv - Microbiology 2019Quote: ... together with F185K at 20 μg/mL diluted in HBS-EP buffer (GE Healthcare) were immobilized on two flow-cells of a CM5 sensor chip by direct amine coupling to 3,000 response units (RU) ...
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bioRxiv - Microbiology 2019Quote: ... and DTT 0.015 mg/mL) and loaded on a mono-Q column (GE Healthcare). The column was washed with TGED buffer and protein was eluted with a linear gradient of TGED buffer with NaCl (0.1 M to 1 M) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded onto a 5 mL HisTrap sepharose HP column (GE Healthcare) equilibrated with buffer A ...
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Increasing phagocytosis of microglia through targeting CD33 with liposomes displaying glycan ligandsbioRxiv - Immunology 2021Quote: ... the pellet was dissolved in 3 ml of 30% Percoll (Percoll PLUS, GE Healthcare) and carefully layered on top of 70% Percoll and immediately centrifuged (650 rcf for 20 min) ...
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bioRxiv - Molecular Biology 2021Quote: ... The Flow-Through was loaded in a 1 ml RESOURCE S column (GE Healthcare), and eluted through a 25 ml gradient (100 mM-1 M NaCl) ...
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bioRxiv - Biophysics 2022Quote: ... the solution was then loaded onto HiTrap HP 5 ml affinity column (GE Healthcare) pre-equilibrated with Buffer A ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was directly loaded onto HiTrap HP 5 ml affinity column (GE Healthcare) pre-equilibrated with Buffer A (50 mM Tris.HCl pH 8 ...
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bioRxiv - Immunology 2021Quote: ... The mix was next applied to a 24 mL Superdex 75 increase (GE Healthcare) column equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was loaded onto a HisTrap HP Sepharose column (5 mL, GE Healthcare) pre-equilibrated in column buffer (25 mM Tris-HCl (pH7.5) ...
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bioRxiv - Biochemistry 2019Quote: ... The cleared lysate was loaded onto a 5 ml HisTrap HP column (GE Healthcare), and was washed with stepwise increases of imidazole in Buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were routinely desalted using 5 mL HiTrap™ desalting columns (GE Healthcare, USA) using desalting buffer (50 mM Tris-HCl pH7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was then passed over a 5 mL HisTrap HP column (GE Healthcare), washed with ten CV of Nickel Wash Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then passed over a 5 mL HisTrap HP column (GE Healthcare), washed with 10 CV of Nickel Wash Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was then passed over a 5 mL HisTrap HP column (GE Healthcare) and washed with 10 CV of Nickel Wash Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... filtered and loaded onto three connected 5 ml HiTrap Q HP columns (GE Healthcare). Washing steps were performed with 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... The clarified supernatant was applied onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated in buffer A connected to an FPLC-Prime (GE Healthcare) ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
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bioRxiv - Biochemistry 2020Quote: ... the filtered medium was loaded on a 5 mL HisTrap Excel column (GE Healthcare) equilibrated with buffer AG.A (500mM NaCl ...
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bioRxiv - Genetics 2020Quote: ... an additional step of purification with a 1 ml HiTrap Talon column (GE Healthcare) was used in between HiTrap Heparin HP (GE Healthcare ...