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Citations for GE Life Sciences :
1601 - 1650 of 4035 citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... The proteins were further purified by size exclusion chromatography (SEC) using Superdex 200 (GE Healthcare). The selected fractions were pooled and concentrated.
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bioRxiv - Biophysics 2022Quote: ... Protein was affinity purified from lysate using a HisTrap HP 5 mL column (GE Healthcare) in binding buffer (50 mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified by immobilised metal affinity chromatography (IMAC) using Ni-Sepharose resin (GE Healthcare) and eluted stepwise in binding buffer containing 40–500 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was applied to a 5-mL Heparin HiTrap column (GE Life Sciences), washed with buffer B (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified via loading onto a 5mL HisTrap HP column (GE Healthcare) and pooling the untagged elution ...
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bioRxiv - Cell Biology 2020Quote: ... and the complex was pulled down using pre-equilibrated Protein A Sepharose beads (GE Healthcare) for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... The purified protein was further polished using a Superdex 200 10/300 GL (GE Healthcare) equilibrated in 25 mM HEPES/NaOH ...
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bioRxiv - Genetics 2020Quote: ... The protein was subsequently purified over a 5 ml HiTrap Heparin HP column (GE Healthcare) and a 1 ml HiTrap Q HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by HiTrap HP cation exchange chromatography (GE Healthcare, Chicago, IL) and Superdex 75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... The tag-free protein was reapplied onto a HisTrap HP 5 ml column (GE Healthcare) to separate the cleaved product from the respective tags and potentially uncleaved protein ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was purified over the gel filtration chromatography (Superdex 200 16/300; GE Healthcare) equilibrated with storage buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... the immunoreactive proteins were visualized using enhanced chemioluminescence plus (ECL+, GE Healthcare, Little Chalfont, England). Densitometric analysis was carried out using Image J software.
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bioRxiv - Biochemistry 2021Quote: ... all protein samples were purified to homogeneity using Superdex 75 prep grade columns (GE Healthcare).
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bioRxiv - Bioengineering 2021Quote: ... The antibody secreted into the culture medium was purified using Protein A resin (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified over a Superdex 75 16/600 pd column (GE Healthcare) equilibrated in 50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... Eluted proteins were further purified over a Superdex 75 16/600 pd column (GE Healthcare) equilibrated in 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was applied to a 5 ml HiTrap SP FF column (GE Healthcare Biosciences) and eluted with a linear gradient of NaCl (25-500 mM) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was detected by using ImageQuant™ LAS 500 imager (GE Healthcare, GENERAL ELECTRIC COMPANY).
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bioRxiv - Biochemistry 2020Quote: ... Resultant protein mixtures were analysed by ion exchange chromatography using HiTrap Q HP (GE Healthcare) and comparable samples from full elution profiles of wild-type and mutant proteins for each KASH binding-partner were analysed by SDS-PAGE ...
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bioRxiv - Bioengineering 2021Quote: ... The proteins were further purified by size exclusion chromatography (SEC) using Superdex 200 (GE Healthcare). The selected fractions were pooled and concentrated again for further use.
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bioRxiv - Bioengineering 2020Quote: All protein constructs were initially purified by IMAC on Nickel Sepharose Excel resin (GE Healthcare). 0.2 µm-filtered supernatants were applied to columns by gravity flow ...
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bioRxiv - Bioengineering 2020Quote: ... DBCO-functionalized nanobody was further refined on the fast-protein liquid chromatography (FPLC, GE Healthcare). DNA reporter conjugation was performed by incubating DBCO-functionalized nanobody (1 eq. ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was removed upon incubation of the target protein with thrombin (GE Healthcare) at room temperature for 16 h ...
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bioRxiv - Biochemistry 2021Quote: ... The phospho-proteins were buffer exchanged using a Superdex75 10/300 GL column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was precleared with beads (Protein G sepharose™ 4 flast flow, GE Healthcare) for 1 hour at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using anti-SERCA2 antibody and protein A-coupled Sepharose beads (GE Healthcare). Beads were washed twice with high salt buffer (50 mM Tris/Cl ...
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bioRxiv - Biophysics 2021Quote: ... The cleaved protein was further purified through a Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Total protein was separated with SDS-PAGE gels and transferred to PVDF membrane (GE Healthcare). Membrane were blocked with 5% BSA in TBST for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by gel filtration (Superdex 200 10/300 GL, GE Healthcare) and concentrated to approximately 1.5 μM (Amicon) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then eluted using an AKTA Purifier 10 FPLC System (GE Healthcare, Fairfield, CT), with a gradient of 100 mM to 1 M NaCl in 20mM Tris buffer containing 5% glycerol ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred on Amersham Protran Premium 0.2 μm nitrocellulose membranes (GE Healthcare, Solingen, Germany) a Bicine/Bis-Tris transfer buffer (25 mM Bicine ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins were transferred to the nitrocellulose membrane (GE Healthcare Life Sciences, Chicago, IL, USA), blocked with 3% BSA for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were purified on a size-exclusion Superdex200 Increase 10/300 GL column (GE Healthcare) in buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified by anion exchange using an HP/Q Sepharose column (GE Healthcare) which was equilibrated with 2 column volumes (120 mL ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein complex was further purified on a HiTrap Heparin HP column (GE Healthcare), followed by size exclusion chromatography on a Superdex 200 10/300 GL equilibrated in 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... the protein supernatant was loaded onto a nickel-nitrilotriacetic acid (Ni-NTA) column (GE Healthcare) containing 0.1 M Ni2+ and equilibrated with the same loading buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was removed from unbound ligand by adsorption to Q-sepharose beads (GE healthcare, USA), the beads were washed ...
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bioRxiv - Biochemistry 2020Quote: ... These proteins were subsequently purified through gel permeation chromatography on Superdex 200 column (GE Healthcare) in Buffer E ...
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bioRxiv - Plant Biology 2020Quote: ... the wild-type and mutated AIPP3-BAH proteins were purified with glutathione-Sepharose (GE Healthcare) and eluted with elution buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were then purified by ion exchange chromatography on a HiTrapSP HP column (GE Healthcare), equilibrated in 20 mM Citrate pH 5.0 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The target proteins were collected and desalted for subjecting MonoQ 4.6/100PE column (GE Healthcare) using Buffer A and C as shown in Fab proteins ...
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bioRxiv - Biophysics 2021Quote: ... The concentrated protein was loaded to an analytical grade size exclusion column (S75, GE Healthcare) for the further purification ...
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bioRxiv - Immunology 2021Quote: ... RBD protein was then captured using His-Trap HP column (GE Healthcare, Wauwatosa, WI, USA) or HiTrap TALON crude column (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... Protein expression induction and purification was carried out similarly with Glutathione Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to Amersham Protran nitrocellulose membranes (0.45 µm pore size, GE Life Sciences) using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Systems Biology 2021Quote: ... SIL-proteins were purified using the Ni-Sepharose High-Performance resin (GE Healthcare Life Sciences) and precipitated using methanol:chloroform:water precipitation in Eppendorf Protein LoBind® tubes ...
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bioRxiv - Molecular Biology 2021Quote: ... The Ni-NTA affinity chromatography purified eLACCO1 protein was further applied on Superdex200pg (GE healthcare) size exclusion chromatography and buffer exchanged to TBS buffer supplemented with 1 mM CaCl2 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then visualized by chemiluminescence using an ECL Western blotting detection system (GE Healthcare) and monitored by using a LAS-3000 imaging system (FujiFilm).
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and protein was eluted by ca 40 units PreScission protease (GE Healthcare) for 4 hours at 4°C under rotation ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were subsequently purified by anion exchange using a Mono Q column (GE Healthcare) in HB buffer (35 mM PIPES-KOH pH 7.2 ...