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Citations for GE Life Sciences :
1551 - 1600 of 4182 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The full-length ectodomain proteins were purified using HisTrap FF column (GE Life Sciences) in elution buffer containing 20 mM sodium phosphate ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive protein was visualized using an enhanced chemiluminescence analysis kit (GE HealthCare, Piscataway, NJ).
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bioRxiv - Biochemistry 2021Quote: ... The eluted proteins were further purified by gel filtration (Superdex 200 column; GE Healthcare) in PBS with 5 mM β-mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant protein was desalted with PD-10 column (SephadexTM G-25M, GE Healthcare), purified using a cation exchange column [HiTrap carboxymethyl (CM ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were buffer-exchanged with PBS pH 7.4 using PD-10 columns (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The protein was then loaded onto a 5 mL HiTrap Q column (GE Healthcare) equilibrated with 50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was run through the HiLoad 16/600 Superdex 200 column (GE Healthcare) in buffer A and dialyzed against 20 mM Tris-HCl (pH 8.0 at 25 °C) ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing the eluted protein were loaded onto a HiPrep desalting column (GE Healthcare) equilibrated with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Intensities of the resultant protein bands were quantified using ImageQuant TL software (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... Immunoreactive proteins were detected with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) using an ImageQuant LAS 500 device (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were then wet-transferred to a 0.2 μm Nitrocellulose membrane (Amersham GE Healthcare) at 150 V for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... and proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 nitrocellulose, GE Healthcare) in transfer buffer (50 mM Tris-OH ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Cancer Biology 2022Quote: ... The BCA assay kit was used to quantify the isolated protein samples (GE Healthcare). The proteins were then heat-denatured and resolved on SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... IgG was isolated using 5 ml HiTrap Protein G High Performance column (GE Healthcare), whereas IgM was isolated using POROS™ CaptureSelect™ IgM Affinity Matrix (ThermoScientific ...
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bioRxiv - Immunology 2022Quote: ... and the antibodies were purified by protein A affinity chromatography (GE Healthcare Life Sciences) as previously described31 ...
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bioRxiv - Biophysics 2019Quote: ... The labelled proteins were further purified by size-exclusion chromatography (Superdex 200, GE Healthcare) using buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... Phosphorylated proteins were visualized using an FLA7000IP Typhoon Storage Phosphorimager (GE Healthcare Life Sciences) and quantified in ImageJ ...
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged proteins were purified with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01), washed and either eluted with glutathione of cleaved with PreScission protease (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Polyclonal antibody 2134 supernatent was purified using a Protein A affinity column (GE Healthcare). Serum was neutralized with 1/10 volume 1 M Tris pH 8 ...
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bioRxiv - Biophysics 2019Quote: ... The diluted protein was applied to a heparin column (HiTrap Heparin HP, GE Healthcare), washed with 20mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was further purified by cation exchange with Source S column (GE Healthcare), eluted with a linear KCl gradient of 0.1 – 1 M KCl ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were then incubated with protein G sepharose beads (GE Healthcare, 17-0618-01) for 2–3 h at 4°C and beads were washed 3 times with diluted lysis buffer (dilution of 1:3 in dH2O ...
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bioRxiv - Plant Biology 2019Quote: ... The secreted protein was purified from the supernatant by Ni2+ (HisTrap Excel; GE healthcare; equilibrated in 50 mM KPi pH 7.6 ...
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bioRxiv - Molecular Biology 2019Quote: ... The diluted protein was applied to a heparin column (HiTrap Heparin HP, GE Healthcare), washed with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were electrophoretically transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 100 V for 180 min on ice in the transfer buffer without SDS/EDTA ...
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bioRxiv - Plant Biology 2019Quote: ... and the separated protein bands were electroblotted onto a polyvinylidene difluoride membrane (GE Healthcare). Different amount of proteins were used for each western blotting ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were electrophoretically transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 100 V for 180 min on ice in transfer buffer [48 mM Tris/HCl ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were electrophoretically transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 90 V for 90 min on ice in transfer buffer [48 mM Tris/HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... The dialyzed protein was resolved on a HiTrap SP FF 5mL column (GE Healthcare) with a linear gradient between buffer A (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... The mAbs were purified using HiTrap Protein A HP column (GE Healthcare, Chicago, IL), followed by size exclusion chromatography (Superdex 200 16/90 ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant fraction containing proteins were loaded on 5ml His-Trap column (GE Healthcare) and eluted with a linear gradient of elution buffer containing 40mM to 500mM Imidazole ...
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bioRxiv - Neuroscience 2020Quote: Gels were silver stained by using the PlusOne Silver Staining Kit (protein, GE Healthcare) to excise the selected spots following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Subsequently proteins were immobilized on a Ni Sepharose 6 Fast Flow resin (GE Healthcare). The resin was incubated 2-4 h at 4°C with 25 nmol of each fluorophore dissolved in labelling buffer 1 (50 mM Tris-HCl pH 7.4-8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Phosphorylation of substrate proteins was visualized using autoradiography (Typhoon 9210; GE Healthcare Life Sciences). Autoradiographs were quantified with the ImageQuant TL Software (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2020Quote: ... The reactive proteins were detected using Image Quant LAS500 software (GE Healthcare Life Sciences).
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bioRxiv - Pathology 2020Quote: ... Proteins were transferred to the Hybond-PVDF-LFP membrane (Amersham, GE Healthcare Life Sciences) and blocked with 5% skimmed milk in 1X Tris-buffered saline ...
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bioRxiv - Cell Biology 2020Quote: ... the protein was buffered into PBS using a NAP5 column (GE Healthcare, Freiburg, Germany).
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bioRxiv - Molecular Biology 2019Quote: ... The protein solution was loaded on a Superdex200 pg 16/60 column (GE Healthcare) and eluted in ClpX SEC buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant was pre-cleared by incubation with protein G sepharose beads (GE Healthcare) on a rotating platform for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then loaded on a HiTrapQ HP anion exchange column (GE Healthcare) and eluted in a gradient from 120–600 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were purified by affinity chromatography using a GSTrap HP affinity column (GE Healthcare) and size exclusion chromatography (HiLoad S200 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins purification procedures were performed using the chromatographic system Akta Purifier (GE Healthcare, USA).
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bioRxiv - Cell Biology 2019Quote: ... The proteins were separated and transferred to PVDF membrane (GE Healthcare Cat No-10600023) using semi-dry transblot apparatus (Hoefer ...
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bioRxiv - Biochemistry 2021Quote: ... and size exclusion chromatography via an ÄKTA fast protein liquid chromatography system (GE Healthcare). Purity of proteins was determined by SDS-polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated by SDS-PAGE and were transferred onto PVDF membranes (GE Healthcare) for immunoblot analysis ...
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bioRxiv - Immunology 2020Quote: ... IgG was purified with a HiTrap MabSelect SuRe protein A affinity column (GE Healthcare) on an AKTA Pure (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... All poly-histidine tagged proteins were purified using the Ni-NTA agarose (GE Healthcare), and eluted with imidazole ...
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bioRxiv - Immunology 2019Quote: ... MAbs were purified over Protein G Sepharose 4 Fast Flow resin (GE Healthcare; 17061802). The antibodies were buffer exchanged into PBS pH 7.4 prior to use in any cell based assays.
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bioRxiv - Neuroscience 2019Quote: ... GST-tagged proteins were captured by incubation with 20 μl glutathione sepharose (GE Healthcare) beads for 30 min at 4°C ...