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Citations for GE Life Sciences :
1551 - 1600 of 3998 citations for Borrelia burgdorferi sensu stricto B31 VlsE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The cleaved protein was further purified by a Superdex 200 16/60 column (GE Healthcare) in 50mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant protein was purified under denaturing conditions over a His-SpinTrap column (GE Healthcare), desalted using a PD SpinTrap G-25 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... and proteins separated by 12% SDS-PAGE were transferred to a nitrocellulose membrane (GE Healthcare) for 70 Vh at 4°C ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined using commercially available 2-D quant kit (GE Healthcare Life Sciences). Sample quality was confirmed using NuPAGE® protein gels stained with fluorescent SyproRuby protein stain (Thermo Fisher).
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected using horseradish peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Piscataway, NJ) and ECL Plus or ECL Prime western blotting detection reagents (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected using horseradish peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Piscataway, NJ) and ECL Plus or ECL Prime western blotting detection reagents (GE Healthcare Life Sciences) ...
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bioRxiv - Plant Biology 2019Quote: ... before tandem Ni-IMAC and gel filtration protein purification steps using an ÄKTAxpress (GE Healthcare) system ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were further purified through HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare) and eluted in buffer containing 20 mM Tris-Cl (pH 7.5) ...
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bioRxiv - Biochemistry 2019Quote: ... N-ChIP chromatin bound to Protein G Sepharose beads (GE Healthcare cat #17-0618-02) were gently washed twice with ice cold 0.5X PBS and spun down for 1 minute at 4°C at 800 rpm ...
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bioRxiv - Cancer Biology 2019Quote: ... We visualized the proteins with the Amersham ECL Western blotting analysis system (GE Healthcare, RPN2109).
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bioRxiv - Biophysics 2019Quote: ... Eluted proteins were concentrated on Amicon concentrators and passed through a superose-6 (GE Healthcare) gel-filtration column in lysis buffer using a Bio-Rad NGC system ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified via loading onto a 5mL HisTrap HP column (GE Healthcare) and pooling the untagged elution ...
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bioRxiv - Cell Biology 2020Quote: ... and the complex was pulled down using pre-equilibrated Protein A Sepharose beads (GE Healthcare) for 1 h ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved protein was subsequently purified by heparin affinity chromatography (HiTrap Heparin HP, GE Healthcare), eluting with a linear gradient of increasing salt from 100 mM - 1 M KCl ...
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bioRxiv - Immunology 2019Quote: IgG purification from individual rabbit serum samples was performed using Protein G columns (GE Healthcare) according to the manufacturer instructions and adjusted to a final concentration of 20 mg/ml in PBS ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were then loaded on a HiLoad 16/600 Superdex 75 pg (GE Healthcare) pre-equilibrated in buffer C (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-G Sepharose 4 Fast Flow and SAv-Sepharose beads were obtained from GE Healthcare Bio-Sciences AB ...
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bioRxiv - Biophysics 2019Quote: ... proteins separated by SDS-PAGE were transferred to a nitrocellulose membrane (GE Healthcare Life Sciences) and probed with primary antibodies anti-5His monoclonal (1:1,000 ...
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bioRxiv - Biophysics 2019Quote: ... Proteins were further purified by gel filtration chromatography using a Superdex 75 column (GE Healthcare) in a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The purified protein was further polished using a Superdex 200 10/300 GL (GE Healthcare) equilibrated in 25 mM HEPES/NaOH ...
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bioRxiv - Immunology 2019Quote: ... was filtered and loaded into a HiTrap protein A column (GE Healthcare 17040301, for IgG) or HiTrap KappaSelect column (GE Healthcare Life Sciences – 17545812 ...
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bioRxiv - Genetics 2020Quote: ... The protein was subsequently purified over a 5 ml HiTrap Heparin HP column (GE Healthcare) and a 1 ml HiTrap Q HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by HiTrap HP cation exchange chromatography (GE Healthcare, Chicago, IL) and Superdex 75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... The tag-free protein was reapplied onto a HisTrap HP 5 ml column (GE Healthcare) to separate the cleaved product from the respective tags and potentially uncleaved protein ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was purified over the gel filtration chromatography (Superdex 200 16/300; GE Healthcare) equilibrated with storage buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... the immunoreactive proteins were visualized using enhanced chemioluminescence plus (ECL+, GE Healthcare, Little Chalfont, England). Densitometric analysis was carried out using Image J software.
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bioRxiv - Biochemistry 2021Quote: ... all protein samples were purified to homogeneity using Superdex 75 prep grade columns (GE Healthcare).
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bioRxiv - Bioengineering 2021Quote: ... The antibody secreted into the culture medium was purified using Protein A resin (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified over a Superdex 75 16/600 pd column (GE Healthcare) equilibrated in 50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... Eluted proteins were further purified over a Superdex 75 16/600 pd column (GE Healthcare) equilibrated in 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was applied to a 5 ml HiTrap SP FF column (GE Healthcare Biosciences) and eluted with a linear gradient of NaCl (25-500 mM) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was detected by using ImageQuant™ LAS 500 imager (GE Healthcare, GENERAL ELECTRIC COMPANY).
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bioRxiv - Biochemistry 2020Quote: ... Resultant protein mixtures were analysed by ion exchange chromatography using HiTrap Q HP (GE Healthcare) and comparable samples from full elution profiles of wild-type and mutant proteins for each KASH binding-partner were analysed by SDS-PAGE ...
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bioRxiv - Bioengineering 2021Quote: ... The proteins were further purified by size exclusion chromatography (SEC) using Superdex 200 (GE Healthcare). The selected fractions were pooled and concentrated again for further use.
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bioRxiv - Bioengineering 2020Quote: All protein constructs were initially purified by IMAC on Nickel Sepharose Excel resin (GE Healthcare). 0.2 µm-filtered supernatants were applied to columns by gravity flow ...
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bioRxiv - Bioengineering 2020Quote: ... DBCO-functionalized nanobody was further refined on the fast-protein liquid chromatography (FPLC, GE Healthcare). DNA reporter conjugation was performed by incubating DBCO-functionalized nanobody (1 eq. ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was removed upon incubation of the target protein with thrombin (GE Healthcare) at room temperature for 16 h ...
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bioRxiv - Biochemistry 2021Quote: ... The phospho-proteins were buffer exchanged using a Superdex75 10/300 GL column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was precleared with beads (Protein G sepharose™ 4 flast flow, GE Healthcare) for 1 hour at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using anti-SERCA2 antibody and protein A-coupled Sepharose beads (GE Healthcare). Beads were washed twice with high salt buffer (50 mM Tris/Cl ...
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bioRxiv - Biophysics 2021Quote: ... The cleaved protein was further purified through a Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Total protein was separated with SDS-PAGE gels and transferred to PVDF membrane (GE Healthcare). Membrane were blocked with 5% BSA in TBST for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... IgGs in supernatants were purified through a HiTrap protein A column (GE Healthcare, Chicago, IL). Antibodies were eluted with 0.1M glycine pH 2.7 ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by gel filtration (Superdex 200 10/300 GL, GE Healthcare) and concentrated to approximately 1.5 μM (Amicon) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then eluted using an AKTA Purifier 10 FPLC System (GE Healthcare, Fairfield, CT), with a gradient of 100 mM to 1 M NaCl in 20mM Tris buffer containing 5% glycerol ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred on Amersham Protran Premium 0.2 μm nitrocellulose membranes (GE Healthcare, Solingen, Germany) a Bicine/Bis-Tris transfer buffer (25 mM Bicine ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins were transferred to the nitrocellulose membrane (GE Healthcare Life Sciences, Chicago, IL, USA), blocked with 3% BSA for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were purified on a size-exclusion Superdex200 Increase 10/300 GL column (GE Healthcare) in buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified by anion exchange using an HP/Q Sepharose column (GE Healthcare) which was equilibrated with 2 column volumes (120 mL ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein complex was further purified on a HiTrap Heparin HP column (GE Healthcare), followed by size exclusion chromatography on a Superdex 200 10/300 GL equilibrated in 20 mM HEPES pH 8.0 ...