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Citations for GE Life Sciences :
1501 - 1550 of 2279 citations for Inositol polyphosphate 1 phosphatase INPP1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Blots were incubated with horseradish peroxidase (HRP)-coupled sheep anti-mouse IgG (GE Healthcare, NA931VS; 1/10000) and (HRP)-coupled goat anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... for 1 min and imaged using the chemiluminescence setting on an Amersham 680 gel imager (GE Healthcare). Blots were adjusted for brightness and contrast using GIMP (GNU Project ...
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bioRxiv - Microbiology 2024Quote: ... were used together with horseradish peroxidase-conjugated sheep α-mouse immunoglobulin G (dilution: 1:2000) (GE Healthcare) as a secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) and separated at 0.25 ml min-1 using an AKTA Pure instrument (GE Healthcare). One ml fractions were collected ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was added to equilibrated glutathione-Sepharose 4B beads (GE Healthcare; 1 ml/100 g cells) and gently rotated for 4 h at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using an AKTA FPLC with 1 mL nickel-charged HisTrap columns (GE Healthcare Life Sciences) for affinity chromatography of the His-tagged proteins ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT at a flow rate of 2 ml/min using an Akta purifier system (GE Healthcare), eluting at approximately 100 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies were incubated for 1 hour at RT and signals were detected with ECL Select (GE Healthcare) and Odyssey Fc (Li-Cor) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... it was passed through Whatman™ Grade 1 filter paper (diameter 150 mm) (GE Healthcare Life Sciences, Ireland), followed by 0.45-μm membrane filtration and then 0.22-μm membrane filtration (ThermoFisher Scientific Ltd) ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was probed with HRP-conjugated anti-mouse or anti-rabbit (1:5,000, GE Healthcare Bio-Sciences) secondary antibody after washing several times with 1X PBS for 45 min at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Biochemistry 2019Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... Concentrated DJ-1/Hsp32 was loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare), pre-equilibrated with 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were solubilized in 1% (w/v) LMNG and proteins were purified using a StrepTrap column (GE Healthcare). The resin was washed in TBS containing 0.01% (w/v ...
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bioRxiv - Genetics 2019Quote: ... Primary antibodies were detected by HRP-conjugated anti-rabbit IgG (GE Healthcare NA934V, Lot 9636020; 1:10000 dilution) or HRP-conjugated anti-mouse IgG (GE Healthcare NA931V ...
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bioRxiv - Microbiology 2021Quote: ... The amplified wild type and mutant PDE12 cDNAs were cloned into plasmid pGEX-6P-1 (GE Healthcare, USA) at the EcoRI and SalI restriction site ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatants were collected by centrifugation and purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... GST-TEV-FICD was then depleted by a 1 h incubation with GSH-Sepharose 4B matrix (GE Healthcare). AMPylation was confirmed as being stoichiometric by intact-protein mass spectrometry (LC-ESI-MS ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μL of plasma was loaded onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA) and left to dry out ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 1 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 20 mM and 40 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT with USP7 proteins purified as described previously 62 using Glutathione Sepharose 4B beads (GE Healthcare). After elution the GST tag was removed under dialysis using 3C protease and the sample fractionated by PorosXQ anion exchange (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Biophysics 2020Quote: ... excess FluoZin-1 dye was removed by passing the sample through a PD-10 desalting column (GE Healthcare) pre-equilibrated with reconstitution buffer.
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bioRxiv - Biophysics 2021Quote: ... the sample was loaded at 2 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were diluted in blocking solution at 1:1000 (Anti-rabbit IgG: GE Healthcare Cat#NA934, RRID:AB_772206) and 1:2000 (Anti-mouse IgG ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D534 was also purified using 1 mL of M2 anti-FLAG affinity column (GE healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: The HMWP2-Cy2 supernatant was loaded at 1 ml/ min onto a 5 mL HisTrap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Filtered lysate was loaded onto a pre-equilibrated 1 mL or 5 mL HisTrap HP column (GE Healthcare) charged with Ni2+ ...
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bioRxiv - Cell Biology 2022Quote: ... followed by anti-rabbit-IgG and mouse-IgG antibodies coupled to HRP (1:2000, NA934, NA931, GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments encoding APC proteins were inserted into pGEX-5X-1 (GE Healthcare Life Sciences, Pittsburgh, PA, USA). For in vitro transcription and translation reaction ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with TBST containing horseradish peroxidase-conjugated secondary antibody (1:1000; GE Healthcare, Pittsburgh, PA) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... the membrane was incubated for 1 hour with horseradish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare, Piscataway, NJ). The target protein on the membrane was detected by Pierce™ ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) and then loaded on a HiTrap Heparin column (CV, column volume 5 ml; GE Healthcare), equilibrated with buffer A ...
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bioRxiv - Cell Biology 2019Quote: ... filtered through a 0.22 µm filter and loaded onto a HiTrap IMAC FF 1 ml column (GE Healthcare) charged with Ni2+ and attached to an AKTA system ...
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bioRxiv - Microbiology 2019Quote: ... The resulting conidial suspension was filtered through Whatman 1 filter paper (Whatman plc, GE Healthcare Life Sciences, UK) to remove hyphae and debris ...
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bioRxiv - Synthetic Biology 2020Quote: The DNA fragment encoding the PreS1 domain (residues 1-56) was cloned into the pGEX-2T (GE Healthcare) plasmid ...
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bioRxiv - Zoology 2020Quote: ... for 1 h at room temperature and developed in ECL (Amersham ECL Select; GE Healthcare, Chicago, IL, USA). Bands were visualized with the MultiImage III FluorChem Q (Alpha Innotech ...
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bioRxiv - Immunology 2020Quote: ... run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare). Antibodies were eluted using 0.1M glycine pH 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were then incubated with anti-Mouse HRP-conjugated secondary antibody (1:5000 of NA931, GE Healthcare) for 1 hour at room temperature followed by three washes with PBST ...
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bioRxiv - Molecular Biology 2019Quote: ... 30 μl 1× TE was added and the probe spun through a G-50 spin column (GE Healthcare) at 400 × g for 2 minutes ...
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bioRxiv - Synthetic Biology 2019Quote: The clean lysate was next loaded onto a HisTrap HP column (HisTrap FF crude 1 ml, GE Healthcare) at 4°C and flow rate of 0.5 ml min−1 ...
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bioRxiv - Cancer Biology 2021Quote: ... This was followed by the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3,000; GE Healthcare, Freiburg, Germany). An electrochemiluminescence assay (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Method 1 (H-P): Genomiphi™ V2 DNA Amplification Kit (Cytiva illustra™, formerly GE Healthcare Life Sciences) was used for whole genome amplification according to manufacture instruction ...
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bioRxiv - Immunology 2020Quote: Buffy coats were diluted [1:3] with PBS and added to Leukosep tubes containing Ficoll-Paque (GE Healthcare). Tubes were centrifuged at 450g for 40 minutes and the resulting buffy layer extracted ...
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bioRxiv - Microbiology 2020Quote: ... Samples (100 uL) at 1 mg/mL were injected and run over a Superdex 200 GL (GE Healthcare) column at a 0.5 ml/min flow rate ...
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bioRxiv - Immunology 2021Quote: ... and Ni Sepharose™ 6 Fast Flow (50 µL per 1 mg digested mAb; GE Healthcare Life Sciences) which were washed twice with PBS before adding to the reaction mixture ...