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Citations for GE Life Sciences :
1501 - 1550 of 1660 citations for Hum ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Physiology 2020Quote: ... Immunoblotting was conducted with specific antibodies and protein bands were visualized using the enhanced chemiluminescence (ECL) Western Blotting Detection System (GE Healthcare, Chalfont St Giles, UK) or the Odyssey infrared detection system (LICOR).
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Immunology 2020Quote: ... and class II molecules were isolated by standard immunoaffinity purification using purified monoclonal antibody 2G11 [65] produced in-house and covalently linked to CNBr-activated Sepharose (GE Healthcare, Chalfont St Giles, UK). MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931, 1:5000; Rabbit IgG HRP Linked Whole Ab-GE Healthcare, #NA934, 1:5000) in 3% milk in TBST ...
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bioRxiv - Cancer Biology 2022Quote: 1 mg lysate protein aliquots were used for immunoprecipitation with optimized amounts of the indicated antibodies and 20 μL of Protein A Sepharose beads (GE Healthcare, Chalfont St. Giles, UK) followed by SDS-Polyacrylamide (Biorad ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, cat. #NA932, dilution 1:10,000), or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were washed three times with TBST for 10 minutes each and exposed to donkey anti-rabbit secondary antibody conjugated to horse radish peroxidase (GE Healthcare; NA934V; LOT: 16976257; 1:10,000) in TBST containing 5% (w/v ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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bioRxiv - Genetics 2020Quote: ... Radiolabeled probes were prepared by random priming of PCR products covering the regions of interest with Megaprime kit (GE Healthcare) in the presence of α-32P dCTP (3000 Ci/mmol) ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were developed using the SuperSignal West Femto kit (Pierce) or fluorescence imaging according to the manufacturer’s protocol with Amersham Imager 600 (GE Healthcare).
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bioRxiv - Genomics 2019Quote: ... The plate and column pools were amplified using the Illustra GenomiPhi HY DNA Amplification Kit (GE Healthcare Life Sciences, UK) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The cleaved linker and 6x-His tag were then removed using a His SpinTrap kit (GE Healthcare # 28-9321-71). Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010 ...
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bioRxiv - Genomics 2020Quote: The total genomic DNA was extracted from a single aphid using Illustra Nucleon Phytopure kit modifying the manufacturer’s protocol (GE Healthcare). The lysis step was performed adding 10 ul of Proteinase K and incubating the sample in a water bath for 2 hours at 55°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was isolated from samples using illustra RNAspin Mini RNA Isolation Kit with RNase-free DNase I (GE Healthcare) according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinants showing intense hybridization signal selected for the plasmid extraction using the IllustraTM plasmidPrep Mini Spin Kit (GE Healthcare). The inserts released by the EcoRI (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The ligated DNA templates were cleaned by an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, U.S.A). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... Protein quantification was performed by gel densitometry measurements using the 1D analysis module of the ImageQuant TL software (v8.1) and the LMW-SDS Marker Kit (GE Healthcare) as standard ...
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Microbiology 2020Quote: ... Pure FasR and FasRF123A were dialysed against 10 mM sodium acetate pH 4.5 and coupled to a CM5 sensor chip using the Amine Coupling Kit (GE Healthcare). Micromolar concentrations of C20-CoA were dialysed against 25 mM Tris.HCl pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... Digestion products were purified with the illustra GFX PCR DNA and gel band purification kit (GE Healthcare, Little Chalfont, UK) and were directionally cloned in the yeast expression vector pYES2 (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... and developed using the Amersham ECL Prime Western Blotting Detection Kit (catalog no. RPN2232; GE Healthcare Australia, Parramatta, NSW, Australia).
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bioRxiv - Plant Biology 2019Quote: ... Proteins were detected using the Roti-Lumin Kit (Carl-Roth, www.carlroth.com) and visualized in the ImageQuant LAS 4000mini (GE Healthcare, www.gehealthcare.com).
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bioRxiv - Microbiology 2019Quote: ... The Illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (Cat. No. 25-6601-96, GE Healthcare Life Sciences, UK) was used for viral genome amplification (expected avg ...
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bioRxiv - Genetics 2019Quote: ... PCR amplicons from each species presenting positive signals for Micropia were separated by 1.5% agarose gel electrophoresis and purified using Illustra GFXTM PCR DNA and Gel Band Purification kit (GE Healthcare) according to the supplier’s specifications ...
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bioRxiv - Microbiology 2021Quote: ... IgG secondary antibody for 1 h at RT and developed in Amersham ECL Western Blotting Detection Kit according to the manufacturer’s instructions (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... pH 4.0 were immobilized on a Biacore CM5 sensor chip (research grade) with the amine coupling kit following the manufacturer’s instructions (GE Healthcare). 1700 resonance units of FBN2-Ct or ADAMTS6-Ct was coupled to the chip for analysis in a Biacore 3000 instrument (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were visualized on agarose gel then purified using PCR DNA and Gel Band Purification kit (GE Healthcare, UK).
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bioRxiv - Microbiology 2022Quote: ... 2006 35 was covalently immobilized on a CM7 Series S sensor chip using an amine coupling reagent kit (GE Healthcare) at the range level of 3,000 to 5,000 response units (RU) ...
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bioRxiv - Biophysics 2024Quote: ... the membranes were washed with TBST buffer and developed with the Amersham ECL Prime Western Blotting Detection kit (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... membranes were finally incubated for 3 min with an enhanced chemiluminescence assay (ECL-Kit, GE Healthcare Life Science, Amersham, UK) and digital images of the responsive bands automatically captured via an imaging system (ChemiDoc MP ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from plated cells or snap-frozen kidneys using the RNAspin Mini kit (GE Healthcare, #25-0500-72) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... G2 and M phases of fertilized mouse eggs using the EllustraTM QuickPrep MicromRNA Purification Kit (GE healthcare UK Limited, UK). The cDNA is synthesized using Prime Script RT reagent Kit with gDNA Eraser (TakaRa) ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNAs were extracted from blood samples and amplified using illustra GenomiPhi V2 DNA Amplification kits (GE healthcare Life Sciences).
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bioRxiv - Microbiology 2023Quote: ... The positive DNA control was produced by random amplification of 2.5 µl DNA from the BAL of patient LA2 using the GenomiPhi HY kit (GE Healthcare), followed by a purification step using the columns of the QIAamp DNA Blood Mini Kit (Qiagen) ...
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bioRxiv - Bioengineering 2023Quote: Phototransduction activity was assessed by measuring the light-induced hydrolysis of endogenous cyclic (c) GMP with an enzyme immunoassay kit (Biotrack EIA system) according to the manufacturer’s instructions (Amersham Bioscience GE Healthcare). Undifferentiated WTCAct or WTCUDC iPSC and cells differentiated into RS were kept in the dark ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were detected with a luminoimage analyzer (AE-6972C; ATTO, https://www.atto.co.jp) using Amersham ECL prime western blotting detection reagent kit (GE Healthcare, https://www.gehealthcare.com), as described previously (Rahman et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times for 10 min in TBS-T and incubated with the secondary horse radish peroxidase (HRP) coupled antibody (GE Healthcare, NXA931V; in a 1:3000 dilution) for two hours under agitation at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... PVDF membranes were subsequently incubated for 1 hour at room temperature with donkey HRP-linked anti-rabbit secondary antibody (1:5000 for FATP1, FATP4, FAT/CD36; 1:10000 for FABPpm, GE Healthcare, Bio-Science, Baie d’Urfe, QC, Canada) or donkey anti-goat secondary antibody (1:5000 for β-actin ...
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bioRxiv - Plant Biology 2020Quote: ... Frozen tissue was ground in liquid nitrogen and total RNA was extracted using the GE Illustra RNA kit (GE Life Sciences), and 2 μg of eluted RNA was used for cDNA synthesis employing iScript (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... All blots were washed 4X with PBS-T and then developed with a Amersham ECL Western Blotting Detection Kit (GE Healthcare).
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bioRxiv - Developmental Biology 2020Quote: ... 5 μg of total RNA was used to synthesize the first-strand cDNA using the First-Strand cDNA synthesis kit (GE Healthcare) with a pd(N)6 primer ...
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bioRxiv - Microbiology 2019Quote: ... One microliter of the RT product was taken as a template for each of the three independent multiple displacement amplification (MDA) reactions performed with the Illustra GenomiPhi V2 kit (GE Healthcare). This was done to compensate for the random amplification bias ...
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bioRxiv - Cell Biology 2019Quote: ... The column was calibrated with protein standards of known molecular weight and stokes’ radius (Low and High Molecular Weight Kit, GE Healthcare) according to manufacturer’s protocol.
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bioRxiv - Genomics 2019Quote: ... Signals were detected by the HyGLO HRP detection kit (Denville Scientific) and exposed to Amersham Hyperfilm ECL (GE Healthcare Life Science).
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bioRxiv - Microbiology 2021Quote: ... Protein size was analyzed using a calibration curve of proteins included in the high molecular weight calibration kit (GE Healthcare, Chicago). To test frozen samples for aggregation ...
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bioRxiv - Biochemistry 2021Quote: ... The oligomerization state was determined by comparison of the elution volumes to the calibration curve of the column (Gel Filtration Calibration Kit, GE Healthcare). The fraction corresponding to the tetradecamer peak was analyzed by intact protein mass spectrometry ...
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bioRxiv - Biochemistry 2021Quote: ... were loaded with 10 μL of Aβ42 oligomer samples alongside the Amersham High Molecular Weight calibration kit for native electrophoresis (GE Healthcare, USA). The gels were run at 4 °C using the electrophoresis system according to the Invitrogen instructions (ThermoFisher Scientific ...