Labshake search
Citations for GE Life Sciences :
1501 - 1550 of 2218 citations for Cow NADH ubiquinone oxidoreductase chain 5 MT ND5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... the sample was treated with 100 mM phosphate buffer pH 7.5 and 50 mM EDTA before purification by gel filtration at room temperature on a Superose-6 Increase column (GE Healthcare) equilibrated with buffer containing 25 mM TRIS pH 8 ...
-
bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a pre-washed/pre-equilibrated CM Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL) on an Äkta explorer FPLC (GE Healthcare ...
-
bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
-
bioRxiv - Biochemistry 2023Quote: ... The clear top phase obtained above a white band was collected and separated over a 5 ml HiTrap Q FF column (GE Healthcare) equilibrated with buffer A ...
-
bioRxiv - Biochemistry 2023Quote: ... A 2-fold molar excess of LF was mixed with 925 pmoles of hexon and incubated on ice for 5 min followed by SEC on Superose 6 increase 10/300 (GE Healthcare) equilibrated with buffer D ...
-
bioRxiv - Cell Biology 2024Quote: ... The eluate was concentrated to a volume of 5 mL and loaded onto a size-exclusion-chromatography column (Superdex 16/60, GE Healthcare); HR3 LBD was eluted in a buffer of 150 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...
-
bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
-
bioRxiv - Cell Biology 2023Quote: ... was applied to a previously equilibrated HiTrap Chelating HP 5 ml column charged with 50 mM CoCl2 and coupled to a GSTrap HP 1 ml column (both GE Healthcare). Buffer A was used for equilibration ...
-
bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to ∼2 µM for cryo-EM sample preparation.
-
bioRxiv - Molecular Biology 2023Quote: ... Imidazole and NaCl present in the Ni-NTA running buffer were eliminated by using a HiTrap Desalting 5 mL (GE Healthcare) column.
-
bioRxiv - Cell Biology 2023Quote: ... Labelling reactions proceeded for 40 mins at 4°C and the excess dye was removed using NAP-5 desalting columns (17-0853-01, GE Healthcare). Nanobodies to be used for confocal microscopy were labelled with Alexa488 (Thermo Fisher ...
-
bioRxiv - Cell Biology 2023Quote: ... and the supernatant was filtered and loaded onto a 5 mL HisTrap HP column (Cytiva) using an AKTA Start (GE Healthcare). The column was washed in buffer containing 50 mM Tris-HCl (pH 8) ...
-
bioRxiv - Neuroscience 2024Quote: Molecular masses of purified gephyrin and variants were detected by analytical size exclusion chromatography (SEC) using a Superose 6 increase 5/10 (GE Healthcare). Typically ...
-
Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... and equal amounts of protein were separated on 5–20% SDS‒PAGE gels (Wako) and transferred to Hybond-P membranes (GE Healthcare). The following primary antibodies and dilutions were utilized ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were centrifuged to remove cell debris at 20,000 x g for 60 min prior to loading of a 5 mL HiTrap SP-FF column on an ÄKTA purifier FPLC (GE Healthcare) equilibrated with Buffer A ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The urea concentration was set to 6 M and the sample was applied to a 5 mL HiTrap QFF anion exchange column (GE healthcare) and was eluted using a 30 CV long gradient from low salt buffer (20 mM Tris pH 8.0 ...
-
bioRxiv - Molecular Biology 2024Quote: ... participants were asked to lay in the supine position for a minimum 5 minutes prior to full-body dual-energy x-ray absorptiometry (DXA) scan (Lunar Prodigy, GE Healthcare). Lean/soft tissue mass (also commonly termed as lean body mass ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were filtered and then pelleted at 300 g for 5 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
-
bioRxiv - Microbiology 2024Quote: ... fully complementary to the fluorescent strand to avoid it re-hybridizing to the quencher strand) and 5 mM ATP (GE Healthcare) in a 100 µL reaction solution containing 10 mM Tris pH 7.5 ...
-
bioRxiv - Biochemistry 2024Quote: ... Gels were fixed with 5% cold trichloroacetic acid and then dried onto chromatography paper (Whatman) and autoradiographed with Amerhsam Hyperfilm-MP (GE Healthcare). Gel images were scanned using a Typhoon phosphorimager (GE Healthcare ...
-
bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...
-
bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 2µM for cryo-EM sample preparation.
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant was then filtered through 0.2 µm PES filter membrane and loaded onto a HisTrap HP column (5 ml, 1.6 x 2.5 cm, Ni Sepharose High-performance column, GE Healthcare, now Cytiva) pre-equilibrated with buffer A at 1 mL/min ...
-
bioRxiv - Microbiology 2024Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
-
bioRxiv - Cancer Biology 2024Quote: ... 60 μl of this mixture was added in the HUVEC side channel of the chip and max intensity projections were taken every 5 minutes for 30 minutes using the IN Cell Analyzer 6000 (GE Healthcare) microscope.
-
bioRxiv - Biophysics 2021Quote: ... using a 1 kDa MWCO Mini Dialysis Kit (GE Healthcare). Finally ...
-
bioRxiv - Genomics 2020Quote: ... Total gDNA was isolated using Nucleon PhytoPure kit (GE healthcare). EVADE DNA copy number was quantified using qPCR with EVADE qPCR primers and single copy gene primers as reference (the primers are listed in Supplemental Table S6) ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by either chemiluminescence detection (ECL detection kit; GE Healthcare) or fluorescence detection (Odyssee ...
-
bioRxiv - Developmental Biology 2019Quote: RNA was collected with the illustra triplePrep kit (GE healthcare) or TRIzol (Invitrogen) ...
-
bioRxiv - Cell Biology 2021Quote: ... Chemiluminescence was detected using ECLplus kit (GE Healthcare, Amersham™) and Azure c500 system (Azure Biosystems ...
-
bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence was detected using ECLplus kit (GE Healthcare, Amersham™) and Azure c500 system (Azure Biosystems ...
-
bioRxiv - Immunology 2021Quote: ... Antibodies were detected using the ECL detection kit (GE Healthcare). Prior to re-probing with different antibodies ...
-
bioRxiv - Immunology 2021Quote: ... by an Amine Coupling Kit (GE Healthcare, BR-1000-50). The interaction between CyPA and CD147 was detected using the kinetic analysis/concentration series/direct binding mode ...
-
bioRxiv - Biochemistry 2022Quote: ... Molecular weight standards (Gel Filtration Calibration kits LMW; GE Healthcare) were used to estimate the molecular weight of Pvs25 by calculating the partition coefficient (Kav ...
-
bioRxiv - Microbiology 2022Quote: ... using the illustra RNAspin Mini RNA Isolation Kit (GE Healthcare) following the “Protocol for total RNA purification from up to 109 bacterial cells,” which includes a 15-minute room-temperature incubation with RNase-free DNase I ...
-
bioRxiv - Cell Biology 2021Quote: ... DNA was purified using a column purification kit (GE Healthcare) and submitted to 5× PyroTaq EvaGreen qPCR Mix Plus (CMB ...
-
bioRxiv - Biochemistry 2019Quote: For amine coupling using the Amine Coupling kit (GE Healthcare), ligand proteins were desalted into immobilization buffer (25 mM potassium phosphate pH 7.5 ...
-
bioRxiv - Biochemistry 2019Quote: For thiol coupling using the Thiol Coupling kit (GE Healthcare), ligand proteins were incubated with 10 mM (dithiothreitol ...
-
bioRxiv - Developmental Biology 2019Quote: ... RNA was extracted using the RNASpin Mini kit (GE Healthcare) according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2019Quote: ... and quantified using 2D Quant kit (GE Healthcare, Piscataway, NJ). Proteins (100μg ...
-
bioRxiv - Plant Biology 2020Quote: ... and Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare) were used for detection and the signal was visualized using the ChemiDoc Touch Imaging System (Bio-Rad).
-
bioRxiv - Plant Biology 2020Quote: ... Immunoreactions were detected by chemoluminescence using different kits (GE Healthcare, Buckinghamshire ...
-
bioRxiv - Immunology 2020Quote: ... Antibodies were detected using the ECL detection kit (GE Healthcare). Prior to re-probing with different antibodies ...
-
bioRxiv - Physiology 2020Quote: ... or ECL™ Prime reagent kits (GE Healthcare, Chicago, IL). Western blots were first probed with the antibody of interest ...
-
bioRxiv - Microbiology 2021Quote: ... Molecular mass standards (Gel Filtration Calibration Kit HMW, GE Healthcare) were run under the same conditions to estimate the size of the complex ...
-
bioRxiv - Microbiology 2021Quote: ... proteins were captured using an amine coupling kit (GE Healthcare), in which the carboxylmethyl dextran matrix of the sensor chip was activated by injection of a mixture of 0.2M 1-ethyl-3-[(3-dimethylamino ...
-
bioRxiv - Microbiology 2022Quote: ... and ECL Prime Western Blotting Detection Kit (GE Healthcare, UK). Loading control was performed with mouse anti α-Tubulin (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2022Quote: ... Detection was performed using Amersham ECL prime kits (GE healthcare) and images were captured using a ChemiDoc imaging system (Bio-Rad) ...