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Citations for GE Life Sciences :
1501 - 1550 of 1724 citations for 6H Perfluorohexene 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The resulting supernatant was applied to a 10-mL column with 1 mL of Ni-Sepharose (GE Healthcare Life Sciences, 17531801) equilibrated with binding buffer (20 mM Tris-HCl ...
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bioRxiv - Systems Biology 2023Quote: ... Isolated cells were washed once in PBS (2mM EDTA, 1% FBS) and lymphocytes were enriched on a 40-67% Percoll gradient (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... was applied to a previously equilibrated HiTrap Chelating HP 5 ml column charged with 50 mM CoCl2 and coupled to a GSTrap HP 1 ml column (both GE Healthcare). Buffer A was used for equilibration ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3x for 10 min with TBST and subsequently incubated for 1 hr at room temperature with appropriate horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) diluted (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used in immunoblotting: HRP-conjugated donkey anti-rabbit IgG (1:2000) (GE Healthcare, catalog number AB_772206), HRP-conjugated goat anti-mouse IgG (1:5000 ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Microbiology 2024Quote: ... were injected first onto a His-Trap crude 1 ml column and further purified by FPLC (Äkta purifier-10, GE Healthcare) using a solution of 50 mM Na-Phosphate pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... the first cDNA strand was generated from 1 µg of the RNA using Ready-to-Go RT-PCR beads (GE Healthcare) and random oligomers ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with secondary antibodies conjugated to different fluorophores (1:750 dilution) and were imaged in the laser-based InCell high-content imaging system (IN Cell Analyzer 6000, GE Healthcare). The nuclear levels of S9.6 or the number of γ-H2AX foci was quantified using the INCarta software that is attached to the imaging system ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies for Western blotting were HRP-linked sheep anti-mouse IgG and donkey anti-rabbit (both GE Healthcare; 1:5000) and HRP-linked mouse anti-rabbit ...
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bioRxiv - Biochemistry 2024Quote: ... Origin substrates were amplified using standard PCR protocols and were purified by anion exchange chromatography using a 1 ml RESOURCE Q column (GE healthcare). Peak fractions were ethanol precipitated and resuspended in TE buffer.
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bioRxiv - Biophysics 2024Quote: ... The filtered supernatant was then incubated at 4 ºC with gentle shaking at 5 rpm for 1 hour in the presence of 5 mL of HisPur Cobalt Resin (GE Healthcare). After multiple rounds of resin cleaning using Extraction Buffer supplemented with 5 mM imidazole ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Microbiology 2024Quote: ... The resulting lysate underwent clarification through centrifugation (125,000g for 30 minutes at 277 K) using a Ti-45 rotor (Beckmann) and was subsequently applied to a 1 ml HisTrap HP column (GE Healthcare). The column underwent an initial wash with five column volumes of lysis buffer containing 40 mM imidazole at pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... Pek1 and Atf1 were generated by PCR of the corresponding gene fragments from yeast genomic DNA or cDNA and cloned in frame into expression vectors pGEX-4T-1 (GE Healthcare) or pMAL-c2x (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were obtained 24 hours after treatment and GST was purified on a 1 mL GSTrap HP column (GE Healthcare) with an AKTA purifier 10 FPLC system ...
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bioRxiv - Plant Biology 2020Quote: ... A peroxidase-conjugated secondary antibody was used at a dilution of 1:5000 and immunoreactive bands were visualized with ECL Western Blotting Detection Reagents (GE Healthcare, UK).
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences). Method for EMSA was modified from a previously published protocol (30) ...
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bioRxiv - Physiology 2022Quote: ... at a dilution of 1:2000 was done for 1 hour at RT in blocking buffer and detection was done using ECL detection kit (RPN2235, GE Healthcare, USA). Images were taken by the ImageQuant LAS500 (GE healthcare ...
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Molecular Biology 2020Quote: ... with Tris-buffered saline/Tween-20 was followed by incubation for 1 h at room temperature with the appropriate secondary antibody (Anti-mouse IgG horseradish peroxidase cat# NA931V from GE Healthcare, Uk). Western blots were developed using the ECL Prime western blotting detection Reagent (RPN 2232 ...
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bioRxiv - Plant Biology 2019Quote: ... containing 0.3 mmol L-1 NH4HCOO (adjusted to pH 3.5 with formic acid) delivered by a Pharmacia 2248 HPLC pump (GE Healthcare, Munich, Germany) were added just after the column via a mixing tee valve ...
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bioRxiv - Biochemistry 2019Quote: The RRM domain of RBFOX2 (amino acids 100-194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28-9546-48) downstream of a GST-SBP tandem affinity tag ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... to a polyvinylidene difluoride membrane and blocked in TBST buffer (TBS with 0.1% (v/v) Tween-20) supplemented with 1% ECL Advance Blocking Agent (GE Healthcare, Waukesha, WI) for 40 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... in the presence of 50 µCi of 32P-α-UTP and 1 mM cold UTP and purified on a Sephadex G25 column (GE Healthcare). Hybridization was performed in the modified Church Buffer at 65°C and the membrane was washed two times 5 min in 2X SSC ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Biochemistry 2019Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... To prevent non-specific binding the surfaces were passivated by injections of 0.2 mg/ml BSA with 1 mg/ml NSB (GE Healthcare Life Sciences) for 120 s prior to a 120 s injection at 30 µl/min of either TtBac-WT ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM EDTA [pH 7.5]) for 2hrs at room temperature and gels were imaged on ImageQuant LAS4010 imager (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2019Quote: ... Virus labeling was performed with Alexa Fluor™A647 succinimidyl ester in a 1:10 molar ratio and was then purified through Nap-5 desalting column (GE Healthcare). Labeled viruses were aliquoted and stored at −80°C ...
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bioRxiv - Immunology 2019Quote: ... Deglycosylated gp12093TH057 coree was first mixed with CD4 mimetic peptide M48U1 at a molar ratio of 1:1.5 and purified through gel filtration chromatography using a Superdex 200 16/60 column (GE Healthcare, Piscataway, NJ). After concentration ...
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bioRxiv - Biophysics 2019Quote: GST-fusion proteins (UBAN domains of OPTN, ABIN, NEMO and Ub) were cloned into pGEX-4T-1 (GE Life Sciences, Freiburg, Germany) and site-directed mutagenesis was performed by PCR to introduce desired amino acid substitutions.
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bioRxiv - Neuroscience 2020Quote: ... and anti-Cav2.3 (ACC-006, Alomone Labs) in combination with peroxidase-coupled secondary donkey anti-rabbit antibodies (NA934, GE Healthcare, 1:10000).
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bioRxiv - Cell Biology 2019Quote: ... The rest was incubated for 1 h at 4°C with 20 µl Ni Sepharose High Performance beads (GE Healthcare Life Sciences) to isolate the nanobodies ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was cleared by centrifugation at 15’000×g for 1 h at 4°C and loaded on a His GraviTrap column (GE Healthcare Life Sciences), washed with 20 mM imidazole in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: donkey anti-rabbit IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK) and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... A 25 mL aliquot was layered over a 1.073 g·mL−1 Ficoll-Paque™ PLUS solution (GE Healthcare, Chicago, Illinois, United States) and centrifuged at 2000 x g for 25 min at room temperature (RT) ...
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bioRxiv - Bioengineering 2021Quote: ... Slides were then washed twice with TBST (TBS with 0.05 % wt/v Tween 20) before incubating with the appropriate biotinylated secondary antibodies (GE Healthcare, 1:500) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Yield can be improved by taking pooled TRAPP complex before SEC and manually running protein through a 1 mL HisTrap™ FF column (GE Healthcare) that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... Protein samples at >1 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2021Quote: ... at 25 mA for 1 h and transferred onto an Amersham™ Protran® 0.2 μm nitrocellulose blotting membrane (GE Healthcare, Canada) at 300 mA for 1.5 h ...
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bioRxiv - Cell Biology 2021Quote: ... human ECs were transfected twice (at 0 and 24 h) with 200 pmol of siGENOME Non-Targeting siRNA #1 (as control) or siGENOME SMART pools siRNA oligonucleotides (GE Healthcare Dharmacon), using Oligofectamine Transfection Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... MKK7 ORF was PCR amplified from Arabidopsis genomic DNA using primers MKK7-F and MKK7-R1 (Table 2) and cloned into pGEX-5X-1 (GE Healthcare, USA) into BamHI-EcoRI restriction sites ...
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bioRxiv - Microbiology 2021Quote: The IgG fraction from the hyperimmunized rabbit serum was purified on 2x 1 mL HiTrap™ Protein G HP columns (GE Healthcare). And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The clear supernatant was filtered (0.22-μm pore size) and loaded onto a 1-ml HisTrap HP column (GE Healthcare Life Sciences) equilibrated with low-KCl buffer A ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was incubated up to 1 h and the non-coupled free dye was removed by exclusion chromatography using a PD10 exclusion column (GE Healthcare, Sweden) equilibrated with 50 mM NaH2PO4/Na2HPO4 with 1% glycerol.