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Citations for GE Life Sciences :
1451 - 1500 of 1713 citations for Rabbit Anti Sheep IgG H+L HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... ON-TARGETplus SMART Pool Non-targeting (D-001810-10) and CHMP2A (L-020247-01) siRNAs were obtained from GE Healthcare Dharmacon ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to Amersham™ Protran® Western blotting membranes, nitrocellulose (pore size 0.2 μm, roll W × L 300 mm × 4 m, pkg of 1 ea) (GE healthcare) overnight 4 °C at 15 V ...
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bioRxiv - Microbiology 2019Quote: ... Samples were incubated at room temperature for 1 h and DNA was extracted with magnetic Sera-Mag Speedbeads (GE Healthcare Life Sciences, UK) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Radioactive gels were exposed to a BAS phosphor-imaging plate (TR2040, Fuji) for 24 h and visualised using a Typhoon scanner (GE Healthcare, FLA-9500).
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was recovered and incubated for 2 h with pre-equilibrated Ni-NTA beads (Ni Sepharose 6 Fast Flow, GE Healthcare Life Sciences) in a binding buffer (50 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... was achieved through incubation at RT for 1 h and subsequent size-exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare, Pittsburgh, USA), equilibrated in storage buffer (50 mM HEPES pH 7.25 ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biochemistry 2023Quote: ... TLC plates were visualized via exposure to a phosphor screen for 16 h prior to imaging using a phosphor screen and Typhoon FLA7000 (GE Healthcare, Pittsburgh PA). Silica gel plates used to assay the metabolism samples and lesion extracts were 150 Å Silica Gel HL 250µ m 10 x 20 cm channeled plates with a pre-adsorbent zone purchased from Analtech/iChromatography (Newark ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified DNA was labelled for 3 h at 15°C with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science).The templates used in this study are ...
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Rhodococcus equi Escapes Macrophage Autophagy: VapA Inhibits ATP6V0d1-Mediated Lysosomal DegradationbioRxiv - Immunology 2023Quote: ... second antibody (1:10000) for 1 h at room temperature and then proteins were visualized with enhance chemiluminescence (ECL) kit purchased from GE Healthcare (Chicago, USA). And detection reagent and analyzed using GE Amersham Imager 600.
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies for rabbit (1:20000 dilution, NA9340; GE healthcare Life science) or mouse (1:20000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were HRP-conjugated and bands were visualized by the ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038–560) and bands were quantified with Gel Doc system (Bio Rad ...
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bioRxiv - Biochemistry 2019Quote: ... and a 1:2500 dilution of the fluorophore-conjugated secondary antibody Cy3 (ECL Plex goat-α-mouse IgG-Cy3, GE Healthcare). Detection was carried out with the Ettan DIGE Imager (GE Healthcare) ...
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bioRxiv - Bioengineering 2020Quote: ... The antibody expression vectors were co-transfected into 293F cells and the cell culture supernatants containing secreted antibody IgG were purified by columns packed in-house containing Protein A Sepharose beads (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... ChIP for Rat1-TAP was performed by incubating 500 μl of the solubilized chromatin sample with 20 μl of IgG Sepharose 6 Fast Flow (GE healthcare) for 3 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... YL½ antibody (IgG, expressed from hybridoma cells and purified by ion exchange chromatography) was coupled to CNBr-activated sepharose beads (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The eluate was diluted with HEP A buffer to 500 mM (NH4)2SO4 and incubated with 20 mL IgG sepharose beads (GE Healthcare), equilibrated by washing with first 0.5 M AcOH pH 3.4 and second 1M Tris-HCl pH 7.5 (RT ...
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bioRxiv - Immunology 2022Quote: ... The Fab fragments are then separated from non-digested IgG and Fc fragments by a 1 hr incubation with rProtein A Sepharose Fast Flow (GE Healthcare). Post incubation ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted at 3500 g and the media containing secreted zRETECD was pooled and 1 ml of IgG sepharose resin (GE Healthcare) was added to 1 l of media and incubated at 4 °C for 18 hrs ...
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting supernatant (∼1 ml) was incubated with 20 µl of dimethyl pimelimidate (DMP) cross - linked IgG Sepharose 6 Fast Flow (GE healthcare) resin for 2 h with gentle rotation at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Afterwards the immobilized papain resin was removed and the digested fraction was mixed with Protein A Sepharose (0.2 ml resin for 1 mg digested IgG, GE Healthcare, #17078001) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... SOSIP proteins were isolated from cell supernatants using a 2G12 immunoaffinity column made by covalently coupling 2G12 IgG monomer to an NHS-activated Sepharose column (GE Healthcare). Protein was eluted with 3M MgCl2 followed by buffer exchange into TBS ...
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bioRxiv - Immunology 2020Quote: ... Secreted Env trimers were isolated using PGT145 immunoaffinity chromatography by covalently coupling PGT145 IgG monomer to an activated-NHS Sepharose column (GE Healthcare) as previously described (81) ...
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bioRxiv - Immunology 2021Quote: The polyclonal IgGs from serum or plasma were purified by incubating with equal volume of Protein A and G sepharose resin (GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared by consecutive centrifugation at 5,000 and 18,000 rpm and incubated with IgG Sepharose 6 Fast Flow beads (GE Healthcare; Cat# 17096902) on a rotating wheel for 120 min at 4 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 4 °C) and the supernatant was incubated with 75 μl of IgG Sepharose 6 Fastbeads (GE Healthcare, 52-2083-00 AH) by rotating at 4 °C for 2 to 3 hours to enable binding of the tagged protein ...
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bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
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bioRxiv - Immunology 2023Quote: ... We separated pFab and Fc from undigested IgG using size exclusion chromatography with a Superdex 200 Increase 10/300 column (GE Healthcare) and concentrated the pFab/Fc.
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bioRxiv - Immunology 2024Quote: IgGs were purified from ∼2 mL of plasma with an equal volume of Protein A Sepharose resin (GE Healthcare Life Sciences) and incubated with the resin for three days at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Cytiva #SH30243.02) supplemented with 10% fetal bovine serum (GE Life Sciences #SH30910.03), 2 mM L-Glutamine (GE Life Sciences #SH30852.01) ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution fractions were loaded onto a SuperoseTM6 10/300 GL column previously equilibrated with buffer L using an ÄKTA Pure system (GE Healthcare). The purified His-SctV9-PR Peptidisc complex was subsequently analyzed by negative-stain electron microscopy.
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bioRxiv - Biophysics 2019Quote: ... 60 - 120 µL of extruded sample were buffer-exchanged through 1.5- to 3.0-mL Sephadex G-50 Fine resin (GE Healthcare, Chicago, IL) columns equilibrated with buffer F (Table 2) ...
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bioRxiv - Molecular Biology 2021Quote: The binding affinity between the purified hPIV3 L-P complex protein and a RNA duplex were measured at room temperature using a Biacore S200 system (GE Healthcare) with a SA chip (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... of the purified hPIV3 L-P complex was determined by analytical size-exclusion chromatography using a Superdex 200 Increase 5/150 GL column (GE healthcare) with buffer B ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by density gradient centrifugation on Ficoll-Paque™ PLUS (1.077 g/L; GE healthcare). Then ...
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bioRxiv - Biochemistry 2019Quote: ... Samples (100 μl) ranging in protein concentration from 15-250 μM were applied to a Superdex 200 10/300 GL column (GE Healthcare) mounted on a Jasco HPLC equilibrated in 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 mg of 3C protease per 3 L of culture volume and further separated on a Superdex 200 HiLoad column (GE Healthcare) in 50mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... 100 μL of this peptide solution was subjected to SEC analysis by passing through Superdex peptide 10/300 GL column (GE healthcare) connected to Bio-Rad (Biologic Duoflow ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... 200μl of the protein mixtures were analyzed by size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare) using a GE ÄKTA FPLC™ (Fast Protein Liquid Chromatograph ...
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bioRxiv - Genetics 2023Quote: Knockdown of mouse Klf4 transcripts was performed with an ON-TARGETplus SMARTpool siRNA against mouse Klf4 (L-040001-01-005, 16600) (Dharmacon, GE Healthcare). This pool of four targeting RNAs targets the following sequences ...
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bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...