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Citations for GE Life Sciences :
1451 - 1500 of 2806 citations for 2 Aminomethyl 1 3 thiazole 4 carboxylic Acid Hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were incubated for 2 h at 25°C and then filtered through a Protran nitrocellulose membrane (Whatman, GE Healthcare Bioscience, Pittsburgh, PA) on top of a Hybond-XL (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... was used according to manufacturer’s guideline to clone cDNAs into pUASTattB (kindly provided by K. Basler, University of Zurich) and pGEX-6P-2 (GE Healthcare Life Sciences). Full length (FL ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted peak fractions were loaded at 2 mL/min in Buffer A onto a 5 mL HiTrap Heparin column (GE Healthcare Life Sciences) and eluted at 2 mL/min with a 50 mL gradient from 30% to 100% Buffer B ...
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bioRxiv - Microbiology 2020Quote: ... The response data were fit to a 1:1 binding model using Biacore™ Insight evaluation software (GE Healthcare). For Fab competition experiments (Figure S8) ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody dilutions in 5% milk/PBST: anti-MBP HRP conjugated, 1:50,000 (NEB, and anti-GST HRP conjugates, 1:2,000 (GE Healthcare,). Scanned western blot films were quantified using the gel analysis tool in ImageJ.
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bioRxiv - Cancer Biology 2021Quote: ... and visualized using HRP-conjugated secondary antibodies (donkey anti-rabbit, 1:15,000, and sheep anti-mouse, 1:15,000, GE Healthcare) and ECL substrate (SuperSignal West Femto Maximum Sensitivity Substrate and Atto Ultimate Sensitivity Substrate ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then blocked for 1 h with TBS-T 1% ECL Prime blocking reagent (GE Healthcare cat # RPN418) at RT on an orbital shaker and blotted with primary antibody for 1 h at RT with gentle agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... blood was diluted 1:1 with PBS and layered on top of 15 mL of Ficoll-Paque (GE healthcare) in 50 mL conical tube ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). The equilibrium dissociation constants (binding affinity ...
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bioRxiv - Microbiology 2021Quote: ... Data were double reference-subtracted and fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Sensorgrams were fitted using 1:1 binding model and kinetic constants determined using Biacore T200 Evaluation software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). To determine the levels of nanobody competition with the human ACE2 and VHH72 of known epitope specificity ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was then incubated on a roller for 1 hr with 1 ml IgG Sepharose resin (GE Healthcare) that had been previously equilibrated in IgG Buffer ...
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bioRxiv - Microbiology 2023Quote: ... and a 1×10 cm strip of Whatman Grade 1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA). As the filter paper was the only carbon source ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunostained embryos were mounted in clearing solution Omnipaque (350 mg litre−1 concentration per 1 ml iohexol, GE Healthcare) and imaged using Leica TCS SP8 DLS microscope as described above ...
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bioRxiv - Biochemistry 2024Quote: ... We used a 1:1 mixture of Sera-Mag Carboxylate-Modified Magnetic Beads (CMMBs; 65152105050250 and 45152105050250, GE Healthcare) to bind the solubilized peptides ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were incubated with HRP-conjugated secondary anitbodies at 1:3,000 for 1 h at room temperature and bands developed using ECL Prime Western Blotting Detection Reagent (GE Healthcare). Blots were subsequently incubated with 1:20,000 HRP-conjugated β-actin (Abcam ...
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bioRxiv - Bioengineering 2020Quote: ... for HRP-conjugated antibodies: donkey monoclonal anti-mouse (1:10000) and donkey monoclonal anti-rabbit antibody (1:10000, GE Healthcare) diluted in 2% BSA-TBST ...
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bioRxiv - Immunology 2020Quote: ... The equilibrium Kd was determined by fitting the data to a steady-state 1:1 binding model using Biacore X100 Evaluation software version 2.0 (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Biochemistry 2020Quote: ... The dialyzed protein was diluted 1:1 with 20 mM HEPES KOH (pH 7.5) and loaded on a HiTrap Heparin HP (GE Healthcare) column and washed with Buffer A (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell culture media was replaced with a 1:1 mixture of 1X HBSS (SH30588.02, GE Healthcare Life Sciences, United States) with 20 mM HEPES (BP310-1 ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... 50-250 μl of plasma were used for immunoglobulin purification via 1:1 mixture of proteinA/proteinG resin (GE Healthcare). Slurry was washed once with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 76 mm × 24 mm super frost glass slides were coated with a solution made of 14:1:1 ethanol:acetic-acid:PlusONE Bind-Silene (GE Healthcare; vol:vol:vol). In parallel ...
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bioRxiv - Neuroscience 2020Quote: ... 1:20,000-1:40,000),immunoreactive bands on the membranes were detected by enhanced chemiluminescence (ECL Plus; GE Healthcare Bio-Sciences). For total protein expression ...
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bioRxiv - Cell Biology 2021Quote: ... Affinity and kinetic constants were determined by fitting the corrected sensorgrams with the 1:1 Langmuir model using BIAevaluation v3.0 software (GE Healthcare). The equilibrium binding constant KD was determined by the ratio of the binding rate constants kd/ka.
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bioRxiv - Microbiology 2020Quote: ... and the kinetic parameters were obtained by globally fitting the double-reference subtracted data to a 1:1 binding model with mass transport limitation using Biacore T200 Evaluation software v 3.1 (GE Healthcare). The dissociation rate constant (kd ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in TBS before a 1 hour incubation with a 1:5,000 dilution of sheep anti-mouse IgG horseradish perodixase secondary antibody (GE Healthcare) in 5% milk-TBS ...
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bioRxiv - Cell Biology 2020Quote: ... 100 µL of CENP-E2055-2608 and CENP-E2055- 2358 at 1 mg·ml-1 were run on a calibrated Superdex-200 10/300 GL Increase (GE Healthcare) size exclusion column pre-equilibrated in gel filtration buffer (described above ...
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bioRxiv - Biochemistry 2022Quote: ... fits of the sensorgrams were determined using a 1:1 binding model (Langmuir) using Biacore T200 Evaluation Software 3.1 (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... and the raw data curves were fitted to a 1:1 binding model using Biacore 8K Evaluation Software (GE Healthcare).
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse, 1/3.000, #NA9310, GE Healthcare). An immune signal was detected using Clarity Western ECL substrate (#1705061 ...
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bioRxiv - Cell Biology 2023Quote: ... was mixed with fibrinogen solution in a 7.5:1 molar ratio for 1 hour at room temperature and then filtered through a HiTrap desalting column (GE Healthcare) packed with Sephadex G-25 resin ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM EDTA and 1 mM DTT) and loaded onto a Mono Q 10/100 GL column (GE Healthcare, Inc.) equilibrated in buffer E and eluted with a 160 mL linear gradient of 0.3–0.5 M NaCl in buffer E ...
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bioRxiv - Biophysics 2023Quote: ... and the binding affinities were obtained using a 1:1 (Langmuir) binding fit model with Biacore T200 Evaluation software version 3.2 (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... All kinetic profiles were fitted using a global 1:1 binding algorithm with a drifting baseline using BIAevaluation v.3.1 (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... 20 μg of beads (1:1 mixture of hydrophilic and hydrophobic Sera-Mag™ carboxylate-modified beads, GE Life Sciences) were added to each sample in 95% ACN ...
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bioRxiv - Biochemistry 2024Quote: ... The elution was then diluted into an equal volume of salt-free buffer (50 mM HEPES, 1 mM TCEP, pH 7.4) and applied to a 1 ml HiTrap Q column (GE Healthcare). The mTORC1 complex and free RAPTOR were separated by a 20 ml salt gradient to a final concentration of 0.5 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The mixture was incubated for 2 hours at RT and antibodies were isolated using illustra™ NAP-5 columns (GE Healthcare Life Sciences™), resulting in an average labeling rate between 0.8 and 1.
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bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated in Buffer B ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein were separated on 4-15 % Tris-glycine (TG) gels (#45101080003-2, SMOBIO, Hsinchu, Taiwan) and electro-transferred onto polyvinylidene fluoride (PVDF) membranes (#A16646282, GE Healthcare, Chicago, IL, USA) in TG buffer ...
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bioRxiv - Immunology 2021Quote: ... The blots were washed in TBS-T and incubated in secondary antibody in antibody buffer for 2 hours before washing again in TBS-T before visualising with ECL (GE Healthcare, Chicago, IL, USA) and imaging in an ALLIANCE 6.7 Chemiluminescence Imaging System (UVITEC ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following the manufacturer’s protocol using 15 µl of PCR product and 2 µl Streptavidin Sepharose High Performance beads (GE Healthcare, cat. GE17-5113-01).
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bioRxiv - Molecular Biology 2023Quote: ... 300 μM 5’ thiol-modified 18-nt DNA oligonucleotides—comprised of an AAA base sequence and a 15-base secondary probe binding sequence (Eurofins)—were reduced with 50 mM dithiothreitol in PBS at room temperature for 2 hours and purified using NAP5 columns (GE Healthcare 17-0853-01). Maleimide-activated antibodies were then combined with 11 equivalents of the reduced form of the thiol-modified DNA oligonucleotides in PBS and incubated at 4℃ overnight ...
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bioRxiv - Immunology 2024Quote: ... Blood was drawn into BD Vacutainer Sodium-Heparin coated tubes and processed within 2 hours by Ficoll density centrifugation (GE Healthcare #17-1440-03).
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bioRxiv - Cell Biology 2020Quote: ... using 1 ml Resource S column (GE healthcare) connected to a nano-HPLC (Ultimate 3000 ...