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Citations for GE Life Sciences :
101 - 150 of 166 citations for TMB Substrates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed with ECL 2 western blot substrate and scanned with an ImageQuant LAS 4000 (GE Healthcare), and signals analyzed with ImageQuant TL 7.0 software (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare). Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce ...
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bioRxiv - Plant Biology 2019Quote: ... Counter-exchange substrate not incorporated into proteoliposomes was removed via gel filtration on Sephadex G-25M columns (GE Healthcare). Transport assays were started either by adding 0.2 mM [α-32P]-phosphoric acid (6,000 Ci/mmol ...
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bioRxiv - Cell Biology 2021Quote: ... After addition of the substrate (Femtoglow by Michigan Diagnostics or ECL Prime Western Blotting Detection Reagent by GE Healthcare), chemiluminescent signals were documented using A BioRad Chemi Doc imaging system ...
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bioRxiv - Cell Biology 2021Quote: ... and developed using BIO-RAD Clarity western ECL substrate (#170-5060) using ImageQuant™ LAS 4000 from GE healthcare Life Sciences.
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bioRxiv - Biophysics 2021Quote: The binding analysis of the substrate analog AMP-PNP was carried out in a Biacore 8K instrument (GE Healthcare) using an NTA Series S sensor chip (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: ... The substrates and products were resolved by 15% urea-denaturing PAGE gel and quantified with Typhoon (Amersham, GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... the membrane was exposed to ECF substrate and imaged using a Typhoon imaging system (GE Life Sciences, Pittsburgh, PA). Band intensities were quantified using ImageJ software (NIH ...
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bioRxiv - Cell Biology 2019Quote: ... Detection of the signal was performed using FluorChem Imaging System (ProteinSimple) with ECL Prime Western Blotting substrate (GE Healthcare) or SuperSignal WestPico PLUS Chemiluminescent Substrate (ThermoScientific).
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bioRxiv - Physiology 2021Quote: ... membranes were incubated for 5 min at room temperature with the Amersham ECL Select peroxidase substrate (RPN2235; GE healthcare), and imaged with the Fusion camera ...
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bioRxiv - Biochemistry 2021Quote: ... HRP-conjugated secondary antibodies were detected with WesternBright ECL chemiluminescent HRP substrate using a ImageQuant imaging unit (GE Healthcare). Band intensities for reference protein standards and neuropil extracts were calculated in ImageJ ...
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bioRxiv - Molecular Biology 2021Quote: ... Western blot was developed using Pierce ECL Western Blotting Substrate (Thermo) and imaged with Amersham Imager 600 (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... Protein-antibody complexes were detected on X-ray films following addition of Enhanced Chemiluminescence (ECL) substrate (GE Healthcare, UK). The relative density of the pCREB and CREB bands was quantitated using ImageJ software (NIH ...
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bioRxiv - Immunology 2022Quote: ... Immunoreactive bands were detected using HRP-conjugated secondary antibodies and revealed with ECL substrate (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Western blot was developed using Pierce ECL Western Blotting Substrate (Thermo) and imaged with Amersham Imager 600 (GE Healthcare).
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were revealed after adding the chemiluminescent substrate using an Amersham Imager 600 device (GE Healthcare, Uppsala, Sweden).
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bioRxiv - Microbiology 2023Quote: ... or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo) to membranes and imaging on an Amersham Imager 600 (GE Healthcare). Membranes were stripped with Restore PLUS Stripping Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were developed by SuperSignal™ West Pico PLUS chemiluminescent substrate (Thermo) using ImageQuant™ LAS 4000 (GE Healthcare). Membranes were stripped with 1X ReBlot Plus Strong Antibody Stripping Solution (#2504 ...
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bioRxiv - Molecular Biology 2021Quote: ... the development was performed using Pierce ECL Western Blotting Substrate (Thermo) and result analyzed with Amersham Imager 600 (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
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bioRxiv - Cell Biology 2020Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with either 1 ml of Amersham ECL substrate western blotting detection reagent (GE Healthcare lot# 9622301) or for higher sensitivity ...
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bioRxiv - Neuroscience 2022Quote: ... Chemiluminescence was detected using ECL substrate and imaged with FlourChem M (Biotechne) or Amersham Imager 600 (GE Healthcare Life Sciences) for membranes from 2D gel ...
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bioRxiv - Cell Biology 2023Quote: ... or with horseradish peroxidase (HRP)-conjugated secondary antibodies followed by a 3-min incubation with a chemiluminescent substrate (GE Healthcare) for ChemiDoc imaging ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were then incubated with SuperSignal West Dura or ProSignal Pico Spray chemiluminescence substrate for 1-4 minutes at room temperature and imaged using an Amersham Imager 600 (GE Healthcare) or ChemiDoc (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... The membrane was removed from the substrates and then exposed to the Amersham imager 600 (GE Healthcare Life Sciences, Marlborough, MA).
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bioRxiv - Microbiology 2020Quote: ... diluted 1:10,000 in TBS-T with 3% BSA and finally washed 3 x in TBS-T for 10 min before adding ECL substrate for detection of HRP activity using a CCD camera (ImageQuant, GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: The kinetic studies of the interaction between the Rubisco activase and the substrate Ac-CoA were performed by the surface plasmon resonance (SPR) method using a Biacore T100 instrument (GE Healthcare) at 25 °C as described (Graciet et al. ...
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bioRxiv - Microbiology 2020Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare). The signal was visualized in a Chemidoc MP imaging system (BIO-RAD).
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bioRxiv - Microbiology 2022Quote: ... or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo-Fisher-Scientific)) and chemiluminescent signals were recorded with an Image Quant LAS4000 imaging system (GE Healthcare) or an Amersham Imager 680RGB (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibody was horseradish peroxidase (HRP)-conjugated anti-mouse IgG or IgA and a chemiluminescence HRP was used as substrate (GE Healthcare). Apparent molecular weight of bands was determined using a standard curve generated from molecular weight markers (r2=0.9916 ...
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bioRxiv - Biochemistry 2020Quote: ... The intensity of the gel bands corresponding to the substrate and the product were quantitated with Typhoon TRIO Phosphorimager (GE Healthcare) using ImageQuant TL software (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed and incubated with horseradish peroxidase (HRP)-conjugated goat-anti-rabbit or donkey-anti mouse secondary antibodies for 1h at room temperature followed by chemiluminescence detection using ECL substrate (Pierce) and imaged using Amersham Imager 600 (GE Healthcare). Densitometric band intensity was determined using Amersham Imager 600 analysis software.
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bioRxiv - Neuroscience 2019Quote: ... The blots were exposed to a peroxidase-conjugated anti-mouse secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare, Piscataway, NJ). The resulting chemilluminescent signal was acquired with a Molecular Imager Chemidoc EQ system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were detected with HRP-conjugated goat anti-mouse or anti-rabbit IgG antibody and visualized with the ECL Western blotting substrate (GE Healthcare), according to the provided protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... or ImmunoStar LD (FUJIFILM Wako Pure Chemical) was used as substrate HRP and luminescent signals were detected using an ImageQuant LAS 4000mini (GE Healthcare). To perform re-probing ...
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bioRxiv - Microbiology 2021Quote: ... blots were incubated with WesternPico Super signal substrate (Pierce/Thermo) for detection and chemoluminescence was visualized with X-Ray films (Hyperfilm, GE Healthcare). The following antibodies were used in this work ...
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bioRxiv - Biochemistry 2021Quote: SPR experiments were carried out to characterize the interaction between PKAc and substrate peptides using a Biacore T200 instrument (GE Healthcare). PKAc was immobilized via standard N-hydroxysuccinimide (NHS ...
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bioRxiv - Biochemistry 2021Quote: ... ubiquitylation of fluorescein-labelled substrates was visualized with a fluorescent scan of an SDS-PAGE gel with a Typhoon imager (GE Healthcare) and quantified with ImageQuant (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were washed and revealed using a commercial kit (Pierce ECL Western Blotting Substrate, Thermo Fischer) and photos were taken by the ImageQuant LAS 4000 mini (GE Healthcare) camera system ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated secondary antibodies were used to incubate the membrane for an hour followed by protein detection with enhanced chemiluminescence (ECL) western blotting substrate and viewed on Amersham Imager 600 (GE Healthcare). Uncropped immunoblot images are shown in Figure S8.
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bioRxiv - Molecular Biology 2022Quote: ... diluted 1:10,000 in TBS-T with 5% powdered milk and finally washed 3x in TBS-T for 10 min before adding ECL substrate (Amersham) for detection of HRP activity using a CCD camera (ImageQuant, GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... the membrane is developed with a chemiluminescent substrate (Super SignalTM West Pico PLUS) and visualized in ImageQuant LAS 4000 mini Biomolecular Imager (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... Finally membranes were again washed three times with TBST and submerged in SuperSignal West Pico chemiluminescent substrate for 2 min before visualizing through ImageQuant LAS 4000 chemiluminescent Image analyzer (GE Healthcare). We used a variety of inhibitors including Torin 1 (inh-tor1 ...
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bioRxiv - Neuroscience 2023Quote: ... Chemiluminescent signals were revealed with SuperSignal West Pico substrate (Pierce) and digitally acquired on a LAS 4000 Mini imaging system (GE Healthcare). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... It was then followed by three times membranes washing in PBST and exposure to either the SuperSignal West Pico PLUS chemiluminescent substrate or the SuperSignal West Femto maximum sensitivity substrate (ThermoScientific) and Images were captured using an ImageQuant LAS4000 mini-Imager (GE Healthcare).
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bioRxiv - Developmental Biology 2022Quote: ... was used as the secondary antibody. Protein was detected using the chemiluminescent substrate ECL (Cat. # 32209, Pierce) in an Amersham Imager 680 (GE Healthcare) detector.
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bioRxiv - Cancer Biology 2023Quote: ... served as chemiluminescent substrates and signals were developed using X-Ray films or imaged on a ProXima 2850 (Isogen) or Amersham 680 (GE healthcare) imaging system ...
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bioRxiv - Developmental Biology 2024Quote: ... Signal detection was obtained with SupersignalTM West Pico Chemiluminescent Substrate HRP and images were acquired using the Amersham Imager 680 RGB (GE Healthcare). Quantifications were performed in Fiji software.