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Citations for GE Life Sciences :
101 - 150 of 337 citations for Sodium hypochlorite solution cp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 10 column volumes of binding buffer (50 mM sodium phosphate, pH 7.2, 500 mM NaCl, 30mM imidazole) using an AKTA FPLC (GE Healthcare, Piscataway, NJ). The column was washed with 15 column volumes of binding buffer and protein was eluted in a linear gradient to 100% elution buffer (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2020Quote: ... The dried butanol fraction was then redissolved in 20mM of sodium phosphate buffer (pH 6) and desalted using a NAP10 desalting column prepacked with Sephadex G-25 resin (GE healthcare, USA). Finally ...
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bioRxiv - Immunology 2021Quote: ... the protein was reduced with DTT and buffer-exchanged into 100 mM sodium phosphate buffer using a Hi-Trap® desalting column (GE Healthcare). Reduced Hypocrates (0.5 μM ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed of unbound probe with a pre-warmed (42°C) 2X saline-sodium citrate (SSC) buffer and detected by phosphor imaging (Storm™ 840, GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... FhuFred was prepared using sodium dithionite and then its excess was removed through buffer exchange in a HiTrap® Desalting Column (GE Healthcare). The different concentrations were used to avoid and deal with the spectra overlap between FhuF and the Fe(III)-siderophores ...
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bioRxiv - Neuroscience 2023Quote: Tissue lysates were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Neuroscience 2024Quote: ... were run on standard 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (pore size 0.45 μm, GE Healthcare Life Sciences). These were then blocked for 1 h with either a 3% BSA solution or 5% milk solution in Tris-buffered saline (20mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... All samples were loaded with the same amount of total protein (40μg) and separated on 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (GE Healthcare, Buckinghamshire, UK) using a Transfer-Blot system (Bio-rad ...
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bioRxiv - Molecular Biology 2023Quote: ... with 20 mM sodium phosphate (pH 6.5) and by size-exclusion chromatography on a HiLoad 16/60 Superdex 75 column (GE Healthcare Life Sciences) with 20 mM sodium phosphate (pH 6.5 ...
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bioRxiv - Cell Biology 2024Quote: ... For western blots protein samples from 1 x 106 trypanosome cells were separated on 12.5 % sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). For protein dot blots ...
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bioRxiv - Biochemistry 2024Quote: ... The latter was achieved by using excess sodium dithionite which was then removed through buffer exchange in a HiTrap® Desalting Column (GE Healthcare). Ferric-siderophore reduction experiments were then performed using 20 μM SbSIPsemi against 100 μM of ferric-siderophore in the stopped-flow apparatus ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each Hb solution was then desalted using a PD-10 column (GE Healthcare) equilibrated with 0.01 M HEPES/0.5mM EDTA (pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... at 10-20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000-4000 response units (RUs) ...
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bioRxiv - Neuroscience 2020Quote: ... in 1X Hank’s Balanced Salt Solution (SH30588.02, GE Healthcare Life Sciences, United States). The dura was removed and the spinal cord was bisected lengthwise ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase activity was detected using enhanced chemiluminescence detection solution (RPN2106, GE Healthcare) and imaged on an SRX-101A X-ray film developer ...
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bioRxiv - Immunology 2022Quote: ... and purified using SpeedBead™ carboxylate magnetic bead solution (GE Healthcare, Marlborough, MA). The amplicons were quantified using KAPA Library Quantitation kit (KAPA ...
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bioRxiv - Neuroscience 2020Quote: ... A 100% Percoll solution was prepared consisting of 90% Percoll (GE Healthcare, UK) and 10% 10x HBSS (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Solutions were sterile-filtered using 0.2 μm syringe filters (GE Healthcare, 6794-2502). 2 ml of each sucrose concentration was layered from 70% to 25% in open-top ultracentrifuge tubes (Beckman Coulter ...
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bioRxiv - Physiology 2020Quote: ... the gradient precipitation using a 30% percoll solution (17-5445-02, GE Healthcare) was performed ...
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bioRxiv - Microbiology 2020Quote: ... the protein solution was applied to a HiTrapTM Q HP column (GE Healthcare) and eluted with linear gradient of 0.15-2.0 M of NaCl in 10 mM Tris (pH 8.0) ...
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bioRxiv - Neuroscience 2023Quote: ... at 10–20 μg/mL concentration in 10 mM acetate solution (GE Healthcare) at pH 4.5 to reach a final surface ligand density of around 2000–4000 response units (RUs) ...
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bioRxiv - Biophysics 2024Quote: ... we loaded the protein solution onto a HiTrap Q FF column (GE Healthcare) and eluted it with a linear gradient of 0–1 M NaCl in 20 mM sodium phosphate at pH 6.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Chemiluminescence detection was performed using ECL Select Peroxide and Luminol solutions (GE Healthcare) according to the manufacturer’s directions.
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bioRxiv - Biophysics 2022Quote: ... the solution was loaded onto a 10 ml Phenyl-sepharose HP (GE Healthcare) column equilibrated with 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... the solution was loaded onto a 10 ml Phenyl-sepharose HP (GE Healthcare) column equilibrated with 20 mM MOPS-NaOH pH 6.7 ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of alkylation solution [100 mM iodoacetamide (GE Healthcare, Chicago, IL, USA)] was added and incubated protected from light for 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... The stained gels were incubated for 30 min with Amplify solution (GE Healthcare), dried and used for 12 to 72-hour fluorography at -80 °C ...
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bioRxiv - Plant Biology 2021Quote: ... 100 µg of freeze-dried HMW compounds were dissolved in 1 mL of 20 mM sodium acetate buffer (pH 4.5) and eluted through a 1 ml HiTrap ANX FF column (GE Healthcare, 17-5162-01). Samples were eluted at a flow rate of 1 ml/min with 20 mM sodium acetate buffer pH 4.5 for 36 ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of prepared protein samples were loaded and separated on 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membrane (GE Healthcare, Buckinghamshire, UK). NZYColour Protein Marker II (NZYTech ...
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bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
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bioRxiv - Biophysics 2022Quote: ... the solution was then loaded onto HiTrap HP 5 ml affinity column (GE Healthcare) pre-equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... The solution was centrifuged and the supernatant loaded to Ni-NTA resin (GE Healthcare) and eluted with buffer E (100 mM Tris at pH 5.8 ...
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bioRxiv - Cell Biology 2021Quote: Chemiluminescent signal was detected by using ECL solution (GE Healthcare, Little Chalfont, Buckinghamshire, UK). Chemiluminescent analysis of immunoblot results was performed by densitometric analysis using LAS400 analyzer (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... PMN were obtained by Ficoll-Paque (PREMIUM solution, GE Healthcare Bio-Sciences AB, Sweden) gradient centrifugation following dextran (Alfa Aesar ...
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bioRxiv - Cell Biology 2021Quote: ... The protein solution was then loaded on an HiTrap IMAC HP column (GE Healthcare). Further purification of the Klp9-containing fractions was achieved by gel filtration using a Superdex 200 26/60 column equilibrated with a buffer containing 20 mM HEPES pH 8 ...
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bioRxiv - Immunology 2021Quote: ... CM5 chip surface was activated by injecting a solution of EDC/NHS (GE Healthcare). Mouse anti-human IgG (Fc ...
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bioRxiv - Cell Biology 2023Quote: ... Fragmented aRNA were added to the hybridization solution (GE Healthcare Europe GmbH, Freiburg, Germany) in a final volume of 260 µl and injected into the CodeLink Uniset Mouse I Bioarrays containing 10,000 mouse genes probes (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... The solution was concentrated using a centrifugal filter (Vivaspin 6-10 kDa; GE Healthcare) to 1 to 2 mg/mL ...
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bioRxiv - Biophysics 2023Quote: ... Leftover NaCl was removed by passing the solution through HiTrap desalting column (GE Healthcare). The UBQLN2 purification protocol here is adapted from [33] ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 pl DNA solution was loaded onto Hybond N+ nylon membranes (GE Life Sciences) presoaked with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... in Hanks’ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with 20 mg/mL DNase (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... The solution was loaded on a HiTrap SP HP cation-exchange column (GE Healthcare). The protein was eluted with a gradient (0-30% ...
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bioRxiv - Microbiology 2023Quote: ... the filtered protein-nanodisc solution was purified using a Superose 6 column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted protein solution was quantified using the 2-D Quant Kit (GE Healthcare) following the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2024Quote: ... DNA solutions were spotted onto Hybond-N+ positively charged nylon membrane (RPN1210B, GE Healthcare). Membranes were blocked at room temperature for 1 hour in TBS-T (Tris-Buffered Saline (TBS) ...
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bioRxiv - Immunology 2024Quote: ... total cell isolates were resuspended in 4 mL of 70% Percoll solution (GE Healthcare), overlaid with 4 mL of 37% Percoll ...
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bioRxiv - Neuroscience 2024Quote: ... 100% Percoll solution was prepared by mixing absolute Percoll (GE Healthcare, #GE17-0891-02) in 10X Dulbecco’s PBS (DPBS ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was diluted 1:1 with 20 mM sodium phosphate pH 7.4 and then purified using HisTrap Excel columns (GE Life Sciences # 17-3712-05) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... They were cultivated in Dulbecco’s Modified Eagle Medium (DMEM/High glucose with L-glutamine, without sodium pyruvate – #SH30022.FS; HyClone, GE Healthcare Life Sciences, Utah, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: ... and were cultured in Dulbecco’s Modified Eagle Medium (DMEM/High glucose with L-glutamine, without sodium pyruvate – SH30022.FS; HyClone, GE Healthcare Life Sciences, Utah, USA) supplemented with 1% penicillin-streptomycin (Gibco ...