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Citations for GE Life Sciences :
101 - 150 of 4180 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... GST-fusion proteins were purified by incubation with glutathione-Sepharose beads(GE Healthcare). The precipitates were washed 3X with lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... GST fusion proteins were immuno-precipitated using Glutathione Sepharose 4B beads (GE Healthcare). Fusion protein-bound beads were used directly in GST pull-down assays ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...
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bioRxiv - Plant Biology 2023Quote: ... GST fusion proteins were purified on Glutathione-Sepharose® 4B beads (GE Healthcare) and eluted using a solution of 50LmM Tris-HCl pH 8 ...
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bioRxiv - Plant Biology 2024Quote: ... GST fusion proteins were purified on Glutathione-Sepharose® 4B beads (GE Healthcare) and eluted using a solution of 50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing GFP and OFP fusion proteins were pulled down with GST-tagged anti-GFP and anti-mCherry nanobodies pre-bound to glutathione– Sepharose-4B beads (GE Healthcare #17075601), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... 1μg of GST or GST-Aurora B or GST-ERK was added to GSH beads (GE Healthcare, USA) in binding buffer (50 mM Tris-cl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... recombinant GST-preS1 was produced in Escherichia coli BL21 cells and purified using glutathione sepharose 4B (GE Healthcare, United States). Ten micrograms of GST or GST fusion proteins and cell lysates were incubated at 4 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... and the GST-fusion protein was purified using Glutathione-Sepharose affinity columns (GE Healthcare). The proteins were finally eluted in PBS containing 20 mM glutathione (pH 8 ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified over Glutathione (GSH) beads (GE Healthcare Biosciences, Pittsburg, PA) in PBS buffer supplemented with protease inhibitors and eluted with 20mM GSH in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified from lysates with glutathione-Sepharose 4 Beads (GE Healthcare). His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant containing GST-hnRNPH2 proteins added to Glutathione Sepharose 4B (GSH; GE Healthcare) beads ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified by binding to Glutathione Sepharose 4B beads (GE Healthcare) and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... The GST fusion proteins were affinity purified via glutathione-sepharose (GE Healthcare, Dornstadt, Germany) by gravity flow ...
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bioRxiv - Molecular Biology 2024Quote: ... The GST fusion proteins were affinity purified using Glutathione Sepharose™ 4B (GE Healthcare) following the batch purification protocol according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... lysates were incubated with purified GST fusion proteins on glutathione-Sepharose beads (GE Healthcare), GFP-trap beads (Chromotek) ...
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bioRxiv - Biochemistry 2022Quote: ... His-GST-ARS2 was applied onto a GST resin (GE Healthcare) and His-MBP-ZFC3H1 onto an Amylose resin (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... GST-OsCOP1 and GST-OsCK2ɑ3 onto GSH- Sepharose (GE Healthcare, USA) by mixing at 4°C for 30 min followed by centrifugation and washing with PBS buffer (Kepinski ...
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bioRxiv - Cell Biology 2022Quote: ... GST-TEAD4-YBD was purified using GST agarose resin (GE Healthcare). GST tag was removed with 3C protease overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare). Recombinant His-tagged PHB1 was incubated with genistein beads (0.5 mg ...
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bioRxiv - Molecular Biology 2023Quote: ... 8.5 μg GST-GST or 8.5 μg GST-BARD1 or 8.5 μg GST-BARD1 + 8.5 μg GST-GST was immobilised on 15 μL Glutathione Sepharose 4B beads (GE Healthcare) in 100 μL pulldown buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... GST-FlgN and GST-FliJ were purified by GST affinity chromatography with a glutathione Sepharose 4B column (GE Healthcare) from the soluble fractions from the Salmonella SJW1368 strain transformed with a pGEX-6p-1-based plasmid as described previously29 ...
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bioRxiv - Biophysics 2020Quote: ... Histidine tagged proteins were immobilized on a Ni2+-sepharose resin (GE Healthcare) to remove the 1 mM DTT (Dithiothreitol ...
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bioRxiv - Biochemistry 2023Quote: ... GST-ICD or ICD-GST (and alanine-scan mutants) and GST-mEGFP were purified through Glutathione Sepharose beads (GE Healthcare), followed by cation exchange chromatography using a Source 15S column (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...
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bioRxiv - Biochemistry 2020Quote: ... anti-GST (GE Healthcare), anti-GAPDH (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was cleaved from the GST tag by incubation with PreScission protease (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
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bioRxiv - Plant Biology 2023Quote: ... these proteins were mixed with GST-LAZY4ΔCCL and incubated with Glutathione Sepharose 4B (GE Healthcare) at 4 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were purified by GST affinity chromatography on the glutathione Sepharose beads (GE Healthcare) in lysis buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were cleaved from the GST resin by incubation with PreScission Protease (GE Healthcare) overnight at 4°C.
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bioRxiv - Microbiology 2020Quote: ... GST-FliJ(Δ13–24) and GST-FlgN were purified by GST affinity chromatography using a glutathione Sepharose 4B column (GE Healthcare) as described previously28 ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant proteins were purified using a HIS-Trap (GE Healthcare) or Amylose resin (New England Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... All other hexa-histidine-tagged proteins were purified using a HisTrap (GE Healthcare) affinity chromatography step followed by thrombin cleavage ...
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bioRxiv - Cell Biology 2022Quote: ... tagged proteins were affinity-purified with a HisTrap HP nickel column (GE Healthcare) or glutathione Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... Tagged proteins were purified by affinity chromatography (HisTrap resp. StrepTrap, both GE Healthcare) with elution buffer containing 200 mM imidazole respectively 2.5 mM d-desthiobiotin ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged Prtg protein was labeled directly with Cy5 monoreactive dye (GE Healthcare). Each bait protein was screened in duplicate against a total number of 1241 secreted proteins distributed across two microarray slides ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using glutathione agarose beads (GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (QIAGEN) ...
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bioRxiv - Biochemistry 2023Quote: ... All the His-tagged proteins were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100 mM-500 mM Imidazole gradients ...
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bioRxiv - Synthetic Biology 2024Quote: ... All 6xHis-tagged proteins were purified using the ÄKTA pure system (GE Healthcare) with a Ni-NTA HisTrap affinity column ...
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bioRxiv - Plant Biology 2022Quote: ... The GST-MIK1CD is loaded onto GST agarose (Glutathione Sepharose 4B, GE Healthcare) and eluted using GST elution buffer containing 50 mM Tris–HCl ...
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bioRxiv - Plant Biology 2023Quote: ... GST and GST-CTU2 were coupled to Glutathione beads (GE Healthcare Life Sciences) and then were incubated with ROL5-His in the binding buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: The human PCM1 C-terminal fragment was cloned into pGEX-2TK GST fusion vector (GE Healthcare Life Sciences) using the primers ...
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bioRxiv - Cell Biology 2020Quote: ... The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences). The beads were washed with binding buffer at least four times ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag fused proteins were purified by affinity chromatography column with GSTrap HP (GE Healthcare) and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... 20 µg of GST fusion protein and 20 µl of Glutathione Sepharose 4B Beads (GE Healthcare) were incubated with 1 ml of HeLa cell extract for 2 h at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The proteins were purified from the cell lysate using 300 μl GST resin (GS4B, GE Healthcare), and incubated with excess purified wild-type or mutant rDPP9 proteins on ice for 60 min ...