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Citations for GE Life Sciences :
101 - 150 of 4264 citations for Recombinant Human IL1F10 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The recombinant proteins were then purified using a 1 ml HisTrap HP column and ÄKTA™ system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant GST-tagged protein in the supernatant was purified using glutathione-Sepharose 4B beads (GE Healthcare, 17-0756-01), washed in 10 mM PBS (pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant catalytic domain of PknB was purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) as previously described5.
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bioRxiv - Epidemiology 2019Quote: Recombinant Schistosoma mansoni glutathione-S-transferase (GST) protein was expressed from pGEX 4T-2 plasmid (GE Healthcare, Piscataway, NJ) and purified as previously described [26] ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: Filtered soluble recombinant wild-type RBPMS-A and mutants were purified on an AKTA protein purification system (GE Healthcare;). Recombinant protein was first purified on HisTrap HP column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing recombinant protein were combined and purified by gel filtration on a S200 26/60 column (GE Healthcare) equilibrated with standard TBS ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Plant Biology 2021Quote: ... Untagged recombinant proteins were then concentrated and further purified by size-exclusion chromatography (Superdex 200 Increase10/300 GL; GE Healthcare) in buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: The recombinant proteins purified as described above were subjected to gel filtration assay (Superdex 200 Increase10/300 GL; GE Healthcare) in buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant RADA and RADA[K201A] proteins were affinity purified in a precalibrated HisTrap FF Crude (GE Healthcare Life Sciences) column run at 0.5 mL/min ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... Recombinant His-tagged proteins (MA4376, MA4376D54N and MA4375) were purified with the ÄKTAprime plus system using HisTrap HP (GE healthcare) columns ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant TSP2 protein was purified from CHO cells expressing mouse TSP2 via affinity chromatography with HiTRAP heparin HP (GE Healthcare) following protocols described by Oganesian et al ...
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bioRxiv - Plant Biology 2022Quote: ... The culture was harvested and the recombinant protein purified using glutathione resin according to the manufacturer’s instructions (GE healthcare, 17075601).
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bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μg of MCF10A cell lysate and 5 μg of individual GST-tagged recombinant proteins were mixed and incubated with glutathione Sepharose 4b beads (GE Healthcare) at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the recombinant proteins were pooled and injected into a size-exclusion chromatography column (Superose12 10/300 GL; GE Healthcare) equilibrated with 50 mM Bis-Tris pH 6.5 ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant protein was finally purified by size-exclusion chromatography (SEC) on a HiLoad 16/60 Superdex 75 column (GE Healthcare) in 10 mM Hepes pH 7.5 and 300 mM NaCl.
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bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Plant Biology 2021Quote: ... the eluates containing recombinant proteins with 10× His-MsyB tag were further purified by an anion-exchange column (Source-15Q; GE Healthcare). The 10×His-MsyB tag was cleaved by TEV protease at 4 °C overnight and removed by an anion-exchange column ...
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bioRxiv - Microbiology 2021Quote: The native molecular mass of the recombinant proteins was determined by gel filtration using a Superdex 200 10/300 column (GE Healthcare), previously calibrated with protein standards (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... The recombinant proteins were purified from supernatants by affinity chromatography with a Ni-agarose column (HisTrapTM HP, GE Healthcare, Chicago, IL), dialyzed against PBS ...
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bioRxiv - Synthetic Biology 2020Quote: The green state of PhoCl1 and PhoCl1 empty barrel recombinant proteins were further purified with size exclusion chromatography using SD200 column (GE Healthcare) and buffer exchanged to 20 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed using a French press and recombinant proteins were purified by nickel ion affinity chromatography (HisTrap HP, 1ml - GE Healthcare). The His6-SUMO tag was removed using Ulp1 protease (Li and Hochstrasser ...
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bioRxiv - Biochemistry 2020Quote: ... Cell extract was clarified by ultracentrifugation at 140 000 x g at 4 °C for 45 min and recombinant CNOT7 protein was purified automatically using ÄKTAxpress FPLC system (GE Healthcare). The following protocol was used for automated purification ...
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bioRxiv - Microbiology 2020Quote: ... the recovered recombinant proteins were further purified by size exclusion chromatography using a Sephacryl S-400 16/60 column (GE Healthcare). Fractions containing the protein were pulled ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
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bioRxiv - Plant Biology 2023Quote: The GST-Tip6ΔSP-mCherry and GST-mCherry recombinant proteins were purified using glutathione sepharose 4B beads (GE Healthcare, Cat. No. 17075601) following the product protocol ...
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bioRxiv - Immunology 2021Quote: ... sterilized by filtration with a 0.4 μm filter and recombinant proteins were purified with HisTrap TALON FF crude or HisTrap Excel columns (GE Healthcare Life Sciences).
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrapTM (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
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bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Microbiology 2021Quote: ... The recombinant RBD was captured by Ni-NTA resin (GE Healthcare) and eluted with 500 mM imidazole in HBS buffer ...
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bioRxiv - Cell Biology 2022Quote: Recombinant GST-NF2 was bound to a GST resin (GE Healthcare), and MBP-NF2 was bound to an MBP resin (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing recombinant was purified on TALON Superflow resin (GE healthcare) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Control human blood samples were processed to remove the most abundant proteins using albumin and IgG depletion SpinTrap columns (GE Healthcare Cat 28-9480-20) following the manufacturers instructions ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...