Labshake search
Citations for GE Life Sciences :
101 - 150 of 4442 citations for Recombinant Human ERN1 Protein GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
-
bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
-
bioRxiv - Immunology 2023Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
-
bioRxiv - Biochemistry 2020Quote: ... Both the His-tagged and GST-tagged variants of PrgA were subsequently loaded on a Superdex- 200 16/600 size exclusion chromatography column (GE Healthcare) in 10 mM HEPES/NaOH (pH 7.5 ...
-
bioRxiv - Systems Biology 2020Quote: ... The supernatant was transferred to a fresh tube and added with 500 μl of 50 % (vol/vol) GST tagged glutathione-sepharose slurry (GE Healthcare) in lysis buffer and rotated at 4 °C for 1 hour ...
-
bioRxiv - Cell Biology 2020Quote: Polyclonal anti-GST antibodies (GST Capture Kit, GE Healthcare) were amine coupled on to a Series S CM5 sensor chip (GE Healthcare ...
-
bioRxiv - Plant Biology 2022Quote: ... HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare, Chicago, IL) at 4 °C for 2 h ...
-
bioRxiv - Microbiology 2021Quote: Expression of recombinant HDP1-DBD motif was done using the Glutathione S-transferase (GST) gene fusion system (GE Healthcare). The pGEX-4T-1 plasmid was used as backbone for cloning the codon optimized sequence comprising the last 87aa of HDP1 ...
-
bioRxiv - Cell Biology 2020Quote: ... freshly purified GST-RILP protein bound to GSH-Sepharose 4B beads (GE Healthcare) was incubated with extracts from transfected HEK293T cells (one 10-cm diameter dish per assay ...
-
bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins were pull-down with glutathione Sepharose 4B beads (GE Healthcare), pre-washed with ice-cold PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... The GST fusion proteins were purified with Glutathione Sepharose ™4B (GE Healthcare) according to the manufacturer’s protocol with Binding Buffer I [50 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2019Quote: ... The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare) was incubated with the Arp2/3 complex in pull-down buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The GST-proteins were purified using Glutathione Sepharose 4B (GE Healthcare Life Sciences) according to the manufacturer’s protocol.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The GST-TTD fusion protein was purified using Glutathione sepharose 4B (GE Healthcare) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... GST-fusion proteins were purified by incubation with glutathione-Sepharose beads(GE Healthcare). The precipitates were washed 3X with lysis buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... GST fusion proteins were immuno-precipitated using Glutathione Sepharose 4B beads (GE Healthcare). Fusion protein-bound beads were used directly in GST pull-down assays ...
-
A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...
-
bioRxiv - Plant Biology 2023Quote: ... GST fusion proteins were purified on Glutathione-Sepharose® 4B beads (GE Healthcare) and eluted using a solution of 50LmM Tris-HCl pH 8 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing GFP and OFP fusion proteins were pulled down with GST-tagged anti-GFP and anti-mCherry nanobodies pre-bound to glutathione– Sepharose-4B beads (GE Healthcare #17075601), respectively ...
-
bioRxiv - Cell Biology 2019Quote: ... GST (GE Healthcare), LC3-II (Cell Signaling) ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant GST-preS1 was produced in Escherichia coli BL21 cells and purified using glutathione sepharose 4B (GE Healthcare, United States). Ten micrograms of GST or GST fusion proteins and cell lysates were incubated at 4 °C overnight ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1μg of GST or GST-Aurora B or GST-ERK was added to GSH beads (GE Healthcare, USA) in binding buffer (50 mM Tris-cl pH 8.0 ...
-
bioRxiv - Microbiology 2019Quote: ... the protein was purified using HisTrap HP histidine-tagged protein columns (GE healthcare, Chicago, IL, USA) according to the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2023Quote: ... The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare). Recombinant His-tagged PHB1 was incubated with genistein beads (0.5 mg ...
-
bioRxiv - Cancer Biology 2021Quote: ... and the GST-fusion protein was purified using Glutathione-Sepharose affinity columns (GE Healthcare). The proteins were finally eluted in PBS containing 20 mM glutathione (pH 8 ...
-
Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare), washed in lysis buffer and kept on beads in lysis buffer with 1 mM DTT at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified over Glutathione (GSH) beads (GE Healthcare Biosciences, Pittsburg, PA) in PBS buffer supplemented with protease inhibitors and eluted with 20mM GSH in PBS pH 7.4 ...
-
bioRxiv - Cell Biology 2021Quote: ... and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare) in 150 mM NaCl ...
-
bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified from lysates with glutathione-Sepharose 4 Beads (GE Healthcare). His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare) ...
-
bioRxiv - Neuroscience 2023Quote: ... and supernatant containing GST-hnRNPH2 proteins added to Glutathione Sepharose 4B (GSH; GE Healthcare) beads ...
-
bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified by binding to Glutathione Sepharose 4B beads (GE Healthcare) and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2022Quote: ... His-GST-ARS2 was applied onto a GST resin (GE Healthcare) and His-MBP-ZFC3H1 onto an Amylose resin (NEB) ...
-
bioRxiv - Plant Biology 2023Quote: ... GST-OsCOP1 and GST-OsCK2ɑ3 onto GSH- Sepharose (GE Healthcare, USA) by mixing at 4°C for 30 min followed by centrifugation and washing with PBS buffer (Kepinski ...
-
bioRxiv - Cell Biology 2022Quote: ... GST-TEAD4-YBD was purified using GST agarose resin (GE Healthcare). GST tag was removed with 3C protease overnight at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 8.5 μg GST-GST or 8.5 μg GST-BARD1 or 8.5 μg GST-BARD1 + 8.5 μg GST-GST was immobilised on 15 μL Glutathione Sepharose 4B beads (GE Healthcare) in 100 μL pulldown buffer (50 mM Tris pH 7.5 ...
-
bioRxiv - Microbiology 2023Quote: ... GST-FlgN and GST-FliJ were purified by GST affinity chromatography with a glutathione Sepharose 4B column (GE Healthcare) from the soluble fractions from the Salmonella SJW1368 strain transformed with a pGEX-6p-1-based plasmid as described previously29 ...
-
bioRxiv - Microbiology 2019Quote: ... soluble 6His-tagged proteins were purified using a NiNTA resine (GE Healthcare) and imidazole was removed by dialysis into a buffer containing 50 mM TrisHCl ...
-
bioRxiv - Biophysics 2020Quote: ... Histidine tagged proteins were immobilized on a Ni2+-sepharose resin (GE Healthcare) to remove the 1 mM DTT (Dithiothreitol ...
-
bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...
-
bioRxiv - Biochemistry 2023Quote: ... GST-ICD or ICD-GST (and alanine-scan mutants) and GST-mEGFP were purified through Glutathione Sepharose beads (GE Healthcare), followed by cation exchange chromatography using a Source 15S column (GE Healthcare ...
-
bioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified from Escherichia coli by glutathione-Sepharose 4B beads (GE Healthcare) and then washed with high salt buffer (20 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein was cleaved from the GST tag by incubation with PreScission protease (GE Healthcare) overnight at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
-
bioRxiv - Plant Biology 2023Quote: ... these proteins were mixed with GST-LAZY4ΔCCL and incubated with Glutathione Sepharose 4B (GE Healthcare) at 4 °C overnight ...
-
bioRxiv - Molecular Biology 2022Quote: ... Recombinant proteins were purified using a HIS-Trap (GE Healthcare) or Amylose resin (New England Biosciences ...
-
bioRxiv - Biochemistry 2020Quote: ... anti-GST (GE Healthcare), anti-GAPDH (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... GST-FliJ(Δ13–24) and GST-FlgN were purified by GST affinity chromatography using a glutathione Sepharose 4B column (GE Healthcare) as described previously28 ...
-
bioRxiv - Biochemistry 2021Quote: ... All other hexa-histidine-tagged proteins were purified using a HisTrap (GE Healthcare) affinity chromatography step followed by thrombin cleavage ...
-
bioRxiv - Cell Biology 2022Quote: ... tagged proteins were affinity-purified with a HisTrap HP nickel column (GE Healthcare) or glutathione Sepharose 4B resin (GE Healthcare ...