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Citations for GE Life Sciences :
101 - 150 of 823 citations for Recombinant Human CEACAM1 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... loaded onto a His-Trap column (GE Healthcare), and eluted by applying a linear imidazole gradient from 20 mM to 200 mM over 30 column volumes ...
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bioRxiv - Biochemistry 2022Quote: ... His-tag was cleaved by thrombin (GE Healthcare) and NaCl concentration was reduced to 100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... and purified using His-Trap HP (GE Healthcare) followed by gel filtration using HiLoad 16/60 Superdex 75 columns on an ÄKTA Xpress ...
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bioRxiv - Molecular Biology 2022Quote: ... NaCl concentration was readjusted to 500 mM by spiking with 5M NaCl stock solution and protein solution was dialyzed overnight into SEC1 buffer at 4°C followed by protein separation from His tag on a His-Trap column (GE Healthcare), concentration with an Amicon Ultra 15 MWCO10kDa (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP and His tags were removed by TEV protease and a passage through a Ni2+chelating Sepharose (GE Healthcare). The final purification step was size-exclusion chromatography on Superdex 200 size exclusion chromatography (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: His-SUMO-SEAL and His-SUMO-SEALGGG were analyzed by size-exclusion chromatography (SEC) on a Superdex S75 column (GE Healthcare) in buffer containing 20 mM of HEPES-NaOH ...
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bioRxiv - Bioengineering 2024Quote: ... and R4E8 were purified using His-tag affinity resin (His GraviTrap or Ni Sepharose 6Fast Flow, GE Healthcare, Chicago, IL, USA). Initially ...
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bioRxiv - Biochemistry 2024Quote: ... His-Rab5 was purified from bacterial cell extracts by passage over a His Trap HP column (GE Healthcare, Fairfield, CT USA) and elution with imidazole according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... GST-tagged TMIGD1 constructs in pGEX-4T-1 (GE Healthcare): GST-TMIGD1 (hTMIGD1 cytoplasmic tail (AA 242-262) ...
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bioRxiv - Cancer Biology 2022Quote: GST-tagged fusion protein purification and PreScission Protease (GE Healthcare) cleavage of pGEX- 6P-1-encoded GST-fusion proteins was performed at 4°C during dialysis against HN buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... 30μM of His-crIFT172968-C or 30μM His-TEV protease were immobilized on 50μL of TALON beads (GE healthcare #28-9574-99) by incubation at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Microbiology 2020Quote: ... were transferred onto a His SpinTrap column (GE Healthcare). The purified fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
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bioRxiv - Immunology 2021Quote: ... functionalised with an anti-His capture kit (GE Healthcare) using a Biacore T200 instrument ...
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bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
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bioRxiv - Biochemistry 2020Quote: ... The GST-tagged constructs were purified with glutathione beads (GE Healthcare), whereas ion metal affinity chromatography using Ni2+ beads (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... GST-tagged Keap1 was purified on glutathione-Sepharose 4B (GE Healthcare), eluted with 10 mM reduced glutathione in 50 mM Tris ...
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bioRxiv - Plant Biology 2021Quote: ... MBP tagged L7TIR was incubated with by PreScission protease (GE Healthcare) at 4°C or 22°C for 12 hr to remove the MBP tag ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged proteins were purified on Glutathione Sepharose resin (GE Healthcare) and eluted with buffer containing 20 mM reduced glutathione ...
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bioRxiv - Cell Biology 2022Quote: ... the GST-tagged protein was purified by Glutathione sepharose (GE Healthcare), followed by on-column TEV cleavage ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2020Quote: ... coli strain BL21 and purified using His-trap (GE Healthcare), Hi-Trap Q (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... was purified and washed on His-Trap columns (GE Healthcare) with buffer Ac (50 mM Tris pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... and purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... A His-Trap crude FF column (GE Healthcare Life Sciences) was used for this purification ...
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bioRxiv - Bioengineering 2021Quote: ... PEGylated mEGFP tagged ferritin was purified with PD10 desalting columns (GE Healthcare) equilibrated with 20mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... GST-tagged proteins were purified using Glutathione Sepharose 4B (GE Healthcare, USA). Protein concentration was determined using Bio-Rad protein assay (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... Histidine tagged proteins were immobilized on a Ni2+-sepharose resin (GE Healthcare) to remove the 1 mM DTT (Dithiothreitol ...
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bioRxiv - Microbiology 2020Quote: ... HisMBP-tagged PurR was loaded onto a HisTrap FF column (GE Healthcare) equilibrated with Lysis Buffer on an AktaPure FPLC (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... and affinity purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Equilibrated a 5 ml His-Trap HP column (GE Life Sciences) with the lysis buffer lacking protease inhibitors ...
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bioRxiv - Biochemistry 2022Quote: ... His-GST-ARS2 was applied onto a GST resin (GE Healthcare) and His-MBP-ZFC3H1 onto an Amylose resin (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl of His Mag Sepharose® Ni beads (GE Healthcare) were equilibrated with equilibration buffer containing 20 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2022Quote: Non-acetylated CobB was purified on His-Trap columns (GE healthcare) as described above however ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the supernatants were mixed with His GraviTrap affinity column (GE Healthcare) and eluted in elution buffer (20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDwt or RBDmut proteins were equilibrated in PBS and kept at −20°C ...
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bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDs proteins were equilibrated in PBS and kept at -20°C.
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bioRxiv - Microbiology 2020Quote: ... The protein was captured using a His-Trap column (GE Healthcare). The column was washed with 10 CV’s of wash buffer ...
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bioRxiv - Biochemistry 2023Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE Healthcare). Histones were eluted by a salt gradient from 200 to 600 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured on Nickel columns (His Trap HP, GE Healthcare) and eluted with 500 mM Imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... All other hexa-histidine-tagged proteins were purified using a HisTrap (GE Healthcare) affinity chromatography step followed by thrombin cleavage ...
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bioRxiv - Plant Biology 2022Quote: ... GST tagged proteins were purified with Glutathione Sepharose 4B beads (10250335, GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged Nlgn1- ECD were purified by rProteinA Sepharose Fast Flow (GE Healthcare), eluted with 10mM glycine ...
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bioRxiv - Cell Biology 2022Quote: ... tagged proteins were affinity-purified with a HisTrap HP nickel column (GE Healthcare) or glutathione Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... Tagged proteins were purified by affinity chromatography (HisTrap resp. StrepTrap, both GE Healthcare) with elution buffer containing 200 mM imidazole respectively 2.5 mM d-desthiobiotin ...
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bioRxiv - Cell Biology 2023Quote: GST-tagged proteins were affinity-purified using glutathione-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using glutathione agarose beads (GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (QIAGEN) ...