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Citations for GE Life Sciences :
101 - 150 of 184 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The dual-labeled heterodimer was then purified by cation exchange chromatography (Mono S 10/100, GE Healthcare) in 25 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Excess P32 gamma-ATP was then separated from labeled AAF DNA by G50 spin column (GE Healthcare). Labeled AAF DNA was mixed 1:4 with unlabeled AAF DNA prior to use for electromobility shift assays (EMSA).
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bioRxiv - Plant Biology 2020Quote: ... Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit; GE Healthcare). Hybridizations were performed at 65°C in Rapid-Hyb buffer (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... H2A.Z(K120C)-H2B-3xFlag and Swc7 were all cysteine-labeled with maleimide-functionalized Cy3 dye (GE Healthcare) using previously published protocols (53) ...
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bioRxiv - Biochemistry 2022Quote: ... Radioactive labeled mitochondrial translation products were detected using Phosphor screens and Amersham Typhoon imaging system (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... a probe was labeled using the Amersham Gene Images AlkPhos Direct Labelling and Detection System (GE Healthcare) according to the manufacturer’s protocol with a DNA fragment generated by PCR using primer pair S01/S02 (Supp ...
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bioRxiv - Biochemistry 2023Quote: ... Radio-labeled analytical gels were dried onto Whatman paper and exposed to Storage Phospho Screens (GE Healthcare). The phosphoscreens were scanned with the Typhoon Trio+ with an excitation wavelength of 633 nm and an emission filter of 390 nm ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled probes were then purified trough a G50 column from the Illustra MicroSpin G50 kit (GE Healthcare) and precipitated overnight with Salmon sperm DNA (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... Cy5-labeled cDNAs were detected by fluorescent mode using a Typhoon FLA 9500 (GE Healthcare Life Sciences) Gel Scanner and LPR Ch.2 filter and 635 nm laser.
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at 4°C overnight prior to removal of unreacted FITC by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The 25 μL sample was then fluorescently labeled with 10 μg Cy3-succinimidyl-ester (GE Healthcare, Buckinghamshire, UK) for 1 h at room temperature in the dark ...
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bioRxiv - Genetics 2020Quote: ... Fluorescently labeled glial cells were scored visually across optical stacks with a Deltavision Core imaging system (Applied Precision) and a PlanApo 60x/1.42 NA oil immersion objective.
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bioRxiv - Immunology 2021Quote: ... Labeled tetramers were purified by size exclusion on a HiPrep 16/60 Sephacryl S–300 column (GE Healthcare). To generate cytometric bead arrays (CBA) ...
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bioRxiv - Cell Biology 2020Quote: ... the labeled protein was separated from the solution by size exclusion chromatography using PD MiniTrap G25 (GE Healthcare). PLCγ1 nSH2-cSH2-SH3 and truncations were labeled with CoA 647 (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP and Input samples were labeled with mono-reactive NHS ester fluorescent Cy5 and Cy3 dyes (GE Healthcare) respectively ...
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bioRxiv - Biophysics 2022Quote: ... Labeled proteins were separated from free dye molecules by gel filtration using a Superdex 200 column (GE Healthcare)56.
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... The BG-labeled oligonucleotide was then purified twice through Illustra G-50 micro columns (GE Healthcare, Buckinghamshire, UK) equilibrated in 2 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... The labeled antibody was separated from free Cy7 using a PD-mini G-25 desalting column (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... the labeled KRAS protein was purified by size-exclusion chromatography using 16/60 Superdex75 increase column (GE Healthcare) pre-equilibrated with 20 mM HEPES (pH 7.0) ...
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bioRxiv - Biophysics 2024Quote: The yeast tRNAPhe from Sigma was labeled on its dihydrouridine (D) residues at the 16/17 positions using Cy3-hydrazide from GE Healthcare. This process involved first reducing the D residues with sodium borohydride (NaBH4 ...
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Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulationbioRxiv - Biochemistry 2023Quote: ... The spin-labeled proteins were further purified by HiLoad 26/600 Superdex 200 pg column (GE life sciences) in a buffer of 150mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The labeled probe was separated from unincorporated nuclides using MicroSpin G-25 columns (GE healthcare, #GE27-5325-01) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... These cells were then visualised using FITC (495/519 nm) and DAPI (358/451 nm) channel using IN Cell Analyzer 6000 (GE Life Sciences) at 40x magnification.
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bioRxiv - Microbiology 2022Quote: ... The FITC-TB_KKG6K bound to specific phosphoinositides was detected fluorometrically with a Typhoon FLA 9500 biomolecular imager (GE Healthcare, Chicago, IL, USA) equipped with a 473 nm laser and filters for excitation/emission wavelengths of 494/520 nm ...
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bioRxiv - Cell Biology 2020Quote: ... 35S-labeled proteins were separated by SDS-PAGE and detected with an Autoimaging analyzer (Typhoon FLA-9500; GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... The labeled proteins were separated from the free dyes on a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer.
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bioRxiv - Biochemistry 2023Quote: ... labeled enzymes were visualized by in-gel fluorescence using a Typhoon FLA 9500 imaging system (GE Healthcare Life Sciences) (Rhodamine channel for probe ...
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bioRxiv - Biophysics 2020Quote: ... which is complementary to the tracrRNA 5’-end was labeled with ATTO647N-NHS (1:60 DNA:dye molar ratio) and purified using G-25 micro spin column (Illustra, GE Healthcare). The measured labeling efficiency of ATTO647N-oligonucleotide was >80% ...
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bioRxiv - Biophysics 2021Quote: ... The dye-labeled protein was purified using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50000 rpm for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were incubated with the appropriate horseradish peroxidase (HRP)-labeled secondary antibody (1:5000 dilution; GE Healthcare UK Ltd, UK). Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo ...
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bioRxiv - Neuroscience 2021Quote: ... NBD-labeled lipids were visualized using an Amersham Typhoon5 imager and quantified by ImageQuantTL (GE Healthcare, Chalfont St Giles, UK). All solvents were of analytical grade and were purchased from Bio-Lab (Jerusalem ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... the labeled proteins were verified by running an SDS-PAGE and scanning using a Typhoon scanner (GE Healthcare, Danderyd, Sweden).
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bioRxiv - Cancer Biology 2021Quote: ... was performed according to a published protocol 63 except that P32-labeled TS primers were used and the signals were quantified on a Phosphor scanner (FLA7000, GE Healthcare). To determine relative telomerase levels between samples ...
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bioRxiv - Biochemistry 2021Quote: ... All labeled and unlabeled G-actins were further purified by size-exclusion chromatography on Sephacryl S200-HR (GE Healthcare, Chicago, IL), stored on ice in G-buffer ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction mixture was incubated at 37°C for 1 h and labeled EVs purified using a PD-10 size exclusion chromatography column (GE Healthcare). Instant thin layer chromatography (iTLC ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction took place for 3 h and the radio labeled kCR probe purified in a Sephadex G25 column (Amersham, GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The gels were exposed to imaging plates and the images of separated tracer-labeled proteins were obtained by reading the plate using a Typhoon 9410 (GE Healthcare) image analyzer ...
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bioRxiv - Biophysics 2021Quote: ... The amount of recovered protein was determined by fluorescence intensity of the labeled FisB ECD band on the gel using a Typhoon FLA 9500 (GE Healthcare). The dissociation constant Kd was determined following ref ...
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bioRxiv - Cell Biology 2020Quote: ... The individual TMT labeled samples were pooled and then fractionated using strong cation exchange chromatography on an AKTA Pure 10 (GE Healthcare) equipped with a Luna 5 μm 100 angstrom 150 x 2.1 mm strong cation exchange (SCX ...
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bioRxiv - Cell Biology 2020Quote: ... Cosmid DNAs that harbor 30-40 kb of sequence around the chosen genomic target were labeled after linearization by nick translation using cy3dUTP (GE Healthcare) as described 93 ...
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bioRxiv - Biophysics 2022Quote: ... After that the labeled protein was purified from the excess of free dye by a PD SpinTrap G-25 column (GE Healthcare), divided into aliquots and stored at -80 °C.
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were visualized using rat anti-HA antibody and detected using peroxidase-labeled anti-rat IgG antibody and ECL Plus (GE Healthcare).
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bioRxiv - Developmental Biology 2022Quote: ... in presence of 32P end-labeled telomeric primers that has been purified using a micro-spin G-25 column (GE healthcare). PCR reactions were resolved by 9% polyacrylamide gel electrophoresis at room temperature ...
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bioRxiv - Physiology 2020Quote: ... The labeled protein was subsequently purified using size exclusion chromatography on a Sephadex™ G-25 Medium column (GE Healthcare, UK). To remove excess free dyes from the labeled proteins and also avoid hydrolysis of extra fluorophore ...
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bioRxiv - Biochemistry 2021Quote: ... A radioactively labeled probe corresponding to the coding region of TbGPI2 was generated using a Megaprime DNA-labeling system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was inactivated at 75 °C for 10 min and the labeled RNA/DNA was purified by gel filtration (Illustra MicroSpin G-25 columns; GE Healthcare). Recombinant ...
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bioRxiv - Cancer Biology 2020Quote: ... the reaction was quenched by addition of the same EDTA solution and the labeled construct was purified using gel-filtration chromatography (Sephadex G-25, PD10 desalting column; GE Healthcare) into 0.9% saline ...
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bioRxiv - Molecular Biology 2021Quote: ... The cap-labeled RNA was then purified from unincorporated nucleotides by using a ProbeQuant G-50 gel filtration column (GE Healthcare).