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Citations for GE Life Sciences :
101 - 150 of 4899 citations for DNA Mismatch Repair Protein Msh3 MSH3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Microbiology 2020Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with corresponding antibody and chemiluminescence was visualized according to the manufacturer’s instructions (ECL Advanced; GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-chromatin complexes were purified with Protein A conjugated to sepharose beads (GE Healthcare, 30 µL GE17-5280-01) for 2 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified using MabSelect Protein A followed by Capto Q according to manufacturer protocols (GE Healthcare, Chicago, IL). Purified antibodies were concentrated and formulated to ≥25 mg/mL in PBS.
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bioRxiv - Biochemistry 2021Quote: GST-MLLE protein was applied to Series S sensor chip CM5 bound with anti-GST antibodies (GE Healthcare BR100223), in Biacore T100 ...
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bioRxiv - Systems Biology 2020Quote: ... Secondary horseradish peroxidase-coupled antibodies (anti-rabbit HRP, anti-goat HRP, Protein A HRP) were purchased from GE Healthcare. Immunoblots were incubated with ECL or ECL advance substrate (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein-antibody complexes were detected on X-ray films following addition of Enhanced Chemiluminescence (ECL) substrate (GE Healthcare, UK). The relative density of the pCREB and CREB bands was quantitated using ImageJ software (NIH ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies (GE Healthcare Life Sciences, Marlborough, MA).
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies (anti-rabbit IgG, anti-mouse IgG or protein-A) coupled with horseradish peroxidase were from GE Healthcare, an anti-goat IgG secondary antibody was from Santa Cruz.
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bioRxiv - Cancer Biology 2023Quote: ... A875 cell extracts were incubated for 16 hr at 4°C on a rotating wheel with 0.8μg of anti-p75NTR antibody (ANT-007, Alomone) and then incubated with Sepharose protein-G beads (GE Healthcare). The beads were collected by brief centrifugation (2 min ...
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bioRxiv - Microbiology 2022Quote: ... IgG1 and IgG2 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... For monoclonal antibody production, the DNA encoding mouse KCTD19 (residue 654-793 aa, NP_808459.1) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies and associated DNA were then isolated using Gammabind G Sepharose beads (GE Healthcare Bio, catalog # 17-0885-01). Crosslinks were reversed by incubating samples and input at 65°C for at least 6 hours ...
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and the others were incubated with the indicated antibodies overnight at 4°C.Then mixed with the Protein G sepharose beads (GE Healthcare) for 2 h at 4°C.The immunoprecipitates were washed four to six times with the 293T lyses buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was collected and the antibodies were purified on a HiTrap Protein-A column (GE healthcare, Little Chalfont, UK).
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bioRxiv - Immunology 2023Quote: IgG was purified from plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Immunology 2023Quote: ... The products were filtered by 10 kDa-cutoff Amicon Ultra centrifugal filters to remove DNA and proteins and then analyzed by a Mono Q 5/50 GL column (GE Healthcare Life Sciences).
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bioRxiv - Cell Biology 2024Quote: ... protein G-Sepharose or protein A-Sepharose beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with secondary antibodies conjugated to horseradish peroxidase (RPN4201 and RPN4301) and the ECL detection kit (both GE Healthcare). Visualization of protein bands was realized by LAS-4000 mini (Fujifilm) ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Neuroscience 2021Quote: ... S9.6 antibodies were purified according to standard procedures (Stork et al., 2016) by using a HiTrap Protein G HP column (GE Healthcare), followed by extensive washing with 20 column volumes of PBS and elution with five column volumes of elution buffer (0.1 M glycine-HCl ...
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bioRxiv - Bioengineering 2020Quote: ... The antibody expression vectors were co-transfected into 293F cells and the cell culture supernatants containing secreted antibody IgG were purified by columns packed in-house containing Protein A Sepharose beads (GE Healthcare).
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bioRxiv - Systems Biology 2020Quote: ... the target-specific antibody was added to the cellular lysates together with 25 μl of Protein A or G sepharose (GE Healthcare) depending on the species of target antibody and the mixture was incubated overnight rotating at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... After incubation with primary antibodies overnight at 4°C followed by the addition of Protein A or G Sepharose beads (GE Healthcare) for 2-3 hrs ...
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bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Biochemistry 2021Quote: The binding affinities of full IgG antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated with rotation at 4°C first for 45 min with antibodies to FLAG and then for 45 min with protein A–Sepharose (GE Healthcare). The resulting immunoprecipitates were subjected to immunoblot analysis.
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bioRxiv - Cancer Biology 2020Quote: ... Simultaneously 3 μg of the anti-FLAG antibody (described above) was subjected to incubation with protein-G sepharose beads (60 ul, 17061801, GE Healthcare) for 1 hour with tumbling ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation was performed with the indicated primary antibodies and 50 μl of a 50% slurry of protein-A sepharose beads (GE Healthcare) for 2 h at 4°C ...
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Immunology 2021Quote: ... All antibody formats were expressed using ExpiCHOTM Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE.
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bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Molecular Biology 2022Quote: ... were used in immunoprecipitation experiments with the anti-Cdc45 rabbit antibodies and control rabbit IgG bound to Protein A Sepharose beads (GE Healthcare). Mixtures were incubated for a minimum of 3 hr (or overnight ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... except that total extracts were subjected to pull-down using monoclonal antibodies against Cherry bound to protein G-Sepharose 4 Fast Flow beads (GE Healthcare). The suspensions were end-over-end mixed on a rotating wheel for 2 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...