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Citations for GE Life Sciences :
101 - 150 of 759 citations for Cow Serine protease HTRA2 mitochondrial HTRA2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... thrombin or PreScission protease and proteins were further purified by ion exchange chromatography (Source 15Q, GE Healthcare) and gel filtration chromatography (Superdex 200 ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved GST-tag and GST-tagged TEV protease was removed by Glutathione Sepharose 4B (GE Healthcare) and the remaining BMAL1 PAS-AB protein was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... the Fc tag was cleaved by O/N incubation with GST-tagged 3C PreScission Protease (GE Healthcare) in cleavage buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... cleaved MBP-6X-His and 6X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Developmental Biology 2022Quote: ... cleaved on the column with 3C protease followed by size exclusion chromatography (HiLoad Superdex 200, GE Healthcare). The final product was collected in 25 mM HEPES pH 7.8 ...
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bioRxiv - Biophysics 2023Quote: ... VD1 was separated from TEV protease and further purified on a HiTrap SP XL column (GE Healthcare). Flow-through fractions containing VD1 were pooled and concentrated to ∼ 1 mM by centrifugation in Amicon Ultra-15 ultrafiltration devices with a 10 kDa molecular weight cut-off (EMD Millipore ...
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bioRxiv - Biophysics 2023Quote: ... and Cdc6 was released from the beads by digestion with Precision protease (GE Healthcare #27-0843-01) at 4 °C for 16 h ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). MBP-CENP-E2605-2701 was purified using the same lysis buffer without imidazole and an MBP-Trap HP coloumn (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... the protein was cleaved with Tev protease and purified on a HiTrap Q HP column (GE Healthcare) using a salt gradient ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were isolated from the purified GST fusion proteins by cleaving with PreScission protease (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... cleaved on the column with 3C protease and followed by size exclusion chromatography (HiLoad Superdex 200, GE Healthcare). The final product was collected in 25 mM HEPES pH 7.8 ...
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bioRxiv - Cell Biology 2019Quote: GST tag was removed from GST-Ana2 by incubating the resin-bound protein with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Microbiology 2020Quote: ... cleavage products from Ulp protease cleavage were separated by additional ion-exchange column (GE healthcare, MonoS G5/50) using a KCl gradient of 0.1M to 1M in MonoS-Sucrose buffer (20% sucrose ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM PMSF and 1x protease-inhibitor cocktail) and loaded onto a Superose™ 6 Increase column (GE Healthcare). Twenty-four fractions of 0.5 mL were collected and precipitated as described (Esteve-Bruna et al. ...
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bioRxiv - Biophysics 2021Quote: ... The TEV-protease cleaved sample was loaded onto a 5 mL Q-Sepharose HP column (GE Healthcare Life Sciences), equilibrated in low-salt buffer and maintained at ∼4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were cleaved overnight with 3C protease followed by gel filtration on Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The GST-tag and Thrombin protease were removed via heparin affinity chromatography (HiTrap Heparin 5 ml FF, GE Healthcare). Cas12a was eluted via an elution gradient with 1 M NaCl (20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... In-gel fluorescence of the protease-protected proOmpA-FM was recorded and quantified (ImageQuant TL, Cytiva/GE Life Sciences). The background signal was subtracted using the implemented Local average algorithm ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine plus MBP tag was removed by TEV protease digestion followed by a second step HisTrap column (GE Healthcare). Protein was further purified using Q and Superdex G75 columns (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
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bioRxiv - Microbiology 2022Quote: ... The TEV protease treated suspension was then transferred into the new columns containing 500 μl Calmodulin Sepharose 4B (GE Healthcare) and 6 ml of CBB buffer [150 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Each GST-fusion protein was released from GST adsorbed to glutathione-sepharose by PreScission protease (GE Healthcare, 27-0843-01). The concentrations of purified proteins were determined by Bradford analysis (Bio-Rad 5000006) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were homogenised by bead beating at 4°C in 500 μl Tris-HCl lysis buffer (100 mM, pH 6.8) containing 1% Protease Inhibitor Mix (GE Healthcare). A full and detailed description of the protocol for 2-dimensional gel electrophoresis (2-DE ...
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bioRxiv - Plant Biology 2019Quote: ... The N-terminal 6xHis tag was cleaved with TEV protease and the protein further purified using a heparin (LUXMYB) or Superdex 200 (LUXFL) column (GE Healthcare).
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bioRxiv - Plant Biology 2019Quote: ... Purification tags were removed by incubation with 3C protease (10 μg/mg fusion protein) followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/60 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare). The target protein was further purified using MonoQ and Superdex G75 columns (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... The solubility tag was cleaved with TEV protease and the protein further purified by size exclusion chromatography (Superdex75 GL300/10/300; GE Healthcare) at 4°C in a SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were digested with PreScission protease to remove tags and further purified by superose-6 increase column (GE Healthcare) in TBS buffer with 0.1% digitonin or 40 μM GDN ...
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bioRxiv - Biochemistry 2020Quote: ... Tag and TEV protease removal was achieved by re-loading the dialyzed sample onto the 5 mL HisTrap Excel column (GE Healthcare), and collecting the unbound tagless sample ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was cleaved with 3C protease overnight and Nes2G-SR11/12 was further purified by cation exchange chromatography on a HiTrap SP-FF column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... It was first purified by a glutathione-affinity step and then subsequent to tag removal following incubation with 3C protease further purified on a MonoS ion-exchange (GE Healthcare) column equilibrated into 25 mM MES pH 6.5 ...
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bioRxiv - Plant Biology 2020Quote: ... The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare) again ...
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bioRxiv - Plant Biology 2021Quote: ... The GST tag was cleaved from purified protein by elution of the tag-free protein from the column by overnight incubation with PreScission Protease (GE Healthcare) or alternatively by elution with 15 mM glutathione-containing purification buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by the addition of PreScission Protease (~100 μL at 76 mg/mL) (GE Healthcare) and the eluted ATAD2B bromodomain protein was concentrated to a total volume of approximately 3 mL ...
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bioRxiv - Biophysics 2021Quote: ... The polyhistidine tag was removed by overnight incubation with TEV protease and further purified by Superdex-75 size-exclusion column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... GST-tag was cleaved with Prescission protease overnight and removed by molecular sieving using an analytical Superdex 75 10/300 chromatography column (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Further polishing of the complex prior SEC and after tag cleavage with PreScission protease was carried out by loading onto a cation-exchange (CE) Resource-S column (GE Healthcare) equilibrated in 10mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was passed through the HisPur resin to remove eGFP-His8 tag and TEV protease and further purified using Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) in SEC buffer containing 0.06% UDM /0.2% DM ...
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bioRxiv - Molecular Biology 2022Quote: ... the gel was washed with the wash buffer and the IF3-bound complexes were eluted by additional incubation of 2 h with the PreScission protease (GE Healthcare) (2 U/μl).
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bioRxiv - Immunology 2022Quote: ... the proteins were treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by a combination of chromatography using HiTrap Heparin (GE Healthcare), Hitrip SP (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The former vector encodes a glutathione S-transferase (GST) fusion protein that can be removed proteolytically with Prescission Protease (GE Healthcare) after affinity purification ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT in the presence of TEV protease and subsequently purified using a 5 mL HiTrap HP Heparin column (GE Healthcare), eluting with a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... was separated from the remaining tag protein and protease by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). The unbound tag-free protein was collected and protein concentration was determined by absorbance (A280 ...
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bioRxiv - Biophysics 2020Quote: ... including an N term extension containing His9-HRV 3C protease site was cloned into the BamHI EcoRI sites in the MCS of pGEX-6P-1 vector (GE Healthcare). Site-directed mutagenesis was performed on the His9-SARS-CoV-2 Nucleocapsid pGEX vector to create the N protein constructs (SI Table XX and sequences were verified using Sanger sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... Ube2V2 and Ube1 were expressed with N-terminal GST-tag followed by a TEV protease cleavage site and purified via glutathione sepharose resin (GE Healthcare) equilibrated in 50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... cleavage was achieved after elution of GbpA from the column by adding 1 unit of PreScission protease enzyme and incubation overnight at 4°C per the instructions of the manufacturer (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... the His6-tag was removed using TEV protease and the protein was subjected to size exclusion chromatography (HiPrep 26/60 Sephacryl S-200; GE Healthcare) using a buffer consisting of 40 mM HEPES pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The liberated affinity-tag and the His-tagged Senp1 protease were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75 or 200 pg ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted proteins were digested with 3C proteases at room temperature and further purified by size exclusion chromatography on HiLoadTM Superdex 75 16/60 prepgrade columns (GE Healthcare) equilibrated with 10 mM Tris-HCl pH 7.5 ...