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Citations for GE Life Sciences :
101 - 150 of 932 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... cleaved on the column with 3C protease and followed by size exclusion chromatography (HiLoad Superdex 200, GE Healthcare). The final product was collected in 25 mM HEPES pH 7.8 ...
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bioRxiv - Molecular Biology 2019Quote: ... Ni-NTA re-capture of the cleaved tag and size-exclusion chromatography (Superdex 75 16/60, GE Healthcare). Timeless 883-947 (DBD C-term ...
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bioRxiv - Biochemistry 2019Quote: ... cleaved proteins were concentrated and finally purified by gel size exclusion chromatography (Superdex G75 26/60, GE Healthcare) in buffer C.
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bioRxiv - Biochemistry 2021Quote: ... Cleaved DEPTOR protein was then collected and applied to a 5 mL HiTrap Heparin HP column (GE Healthcare) equilibrated with HEP-A buffer (50 mM HEPES pH 8 ...
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bioRxiv - Neuroscience 2019Quote: ... Cleaved protein was concentrated (50 kDa MWCO) and run on a Superdex 200 10/300 column (GE Healthcare) in SEC buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleaved Cas9 protein was separated from fusion tag on HiLoad Superdex 200 16/60 column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting tag-cleaved proteins were purified by ion exchange chromatography with a HiTrap SP column (GE Healthcare) and gel filtration with the final buffer (25 mM Tris-HCl buffer at pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The tag cleaved Ub was further purified by size exclusion chromatography through a Superdex 75 column (GE Healthcare) in buffer A.
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bioRxiv - Microbiology 2024Quote: ... Cleaved GFP and desthiobiotin were removed by anion exchange chromatography on a HiTrap Q HP column (GE Healthcare) connected to a FPLC system (ÄKTA purifier-10 ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... The flow-through containing the cleaved eIF2β was pooled and applied to a Superdex S200 column (GE Healthcare) equilibrated in a buffer containing 150 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... the protease was removed from (un)cleaved target protein using a HisTrap FF column (GE Healthcare Life Sciences). Remaining nucleic acids were removed using anion exchange chromatography (AIEX ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant proteins were purified using a HIS-Trap (GE Healthcare) or Amylose resin (New England Biosciences ...
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bioRxiv - Immunology 2021Quote: ... the mAb was purified using a 1 mL HiTrap Protein A HP column (GE Healthcare). The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... mAb followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2,000, NXA931, GE Healthcare). Periplasmic fractions ...
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bioRxiv - Cancer Biology 2019Quote: ... or anti-phospho hTERT mouse mAb (TpMab-1) and anti-mouse IgG HRP (GE Healthcare) were used for IB to detect phosphorylated hTERT proteins.
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified from serum-free culture supernatants using mAb Select SuRe resin (GE Healthcare) followed by rapid desalting using a HiPrep 26/10 Desalting column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Purification from serum-free culture supernatants was done using mAb Select SuRe resin (GE Healthcare) followed by rapid desalting using a HiPrep 26/10 Desalting column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... Cleaved protein was filtered (0.45 µm) and further purified by size-exclusion chromatography (Superdex S200 26/60; GE Healthcare) in buffer containing 20 mM Tris pH 7.5 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The cleaved protein was then concentrated to 2.0 ml final concentration and loaded on the S200 column (GE healthcare). S200 size exclusion chromatography has yielded a single peak around 230 KDa ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B and the cleaved topo VI collected in the flow through ...
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bioRxiv - Biochemistry 2020Quote: ... The cleaved Hsf1 was further separated by size-exclusion on Superdex™200 HiLoad 16/60 column (GE Healthcare), equilibrated with Hsf1 SEC buffer (25 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... KIX domain was cleaved from GST by incubation of resin-bound GST-KIX with 160 U thrombin (GE Healthcare) overnight at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4 °C in 50 mmol L−1 Hepes at pH 8 ...
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bioRxiv - Cell Biology 2021Quote: ... The cleaved protein was further purified through SEC in a Superose 6 increase 10/300 GL column (GE Healthcare), in buffer containing 50 mM phosphate pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... The TEV-protease cleaved sample was loaded onto a 5 mL Q-Sepharose HP column (GE Healthcare Life Sciences), equilibrated in low-salt buffer and maintained at ∼4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The histidine-tagged cleaved proteins where then loaded onto a 26/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with buffer C ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were cleaved overnight with 3C protease followed by gel filtration on Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved CHD4-C1bC2a was further purified by size exclusion chromatography on a Superdex 75 10/300GL column (GE Healthcare) and eluted with 20 mM HEPES pH 7.6 ...
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bioRxiv - Microbiology 2019Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 50 mM Hepes pH8 ...
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bioRxiv - Microbiology 2022Quote: ... The cleaved protein was collected and further purified using gel filtration (HiLoad 16/60 Superdex pg 200, GE Healthcare) with GF buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant containing the cleaved protein was removed and applied to a size exclusion (SEC) column (HiLoad_16/60_Superdex75 prep- grade, GE Healthcare) equilibrated with PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Both tagged and cleaved BtuG samples were then loaded on a NAP-10 desalting column (kinetic assays, GE healthcare) for buffer exchange to 20 mM Tris/HCl pH 7.5 and 100 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... uncleaved protein as well as cleaved GST were removed by applying everything onto 5 ml SP column (GE Healthcare) equilibrated with 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved KRas samples were further purified by SEC using a gel-filtration column (GE Healthcare, HiLoad Superdex 75) in the GF buffer (20 mM Tris-Cl (pH 7.65) ...
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bioRxiv - Microbiology 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 20 mM Hepes pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 50 mM Hepes pH 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved receptor was separated from eGFP by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with m7-glycerol+ buffer containing 6.7 mM DMCH ...
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bioRxiv - Cancer Biology 2023Quote: ... The reaction was run out on a 15% TBE-urea acrylamide gel to separate substrate oligo from cleaved product oligo and imaged on a Typhoon FLA 7000 with ImageQuant TL 8.2.0 software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Recombinant proteins were purified with a HisTrapTM HP (GE Healthcare, Sweden) by an AKTA pure system (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant RBD was captured by Ni-NTA resin (GE Healthcare) and eluted with 500 mM imidazole in HBS buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant expressed protein was purified using HisTrap column (GE Healthcare). The His-Sumo tag was digested by ulp1 protease and removed by a second HisTrap column ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant protein was purified using HiTrap DEAEFF (GE Healthcare, Chicago IL), HisTrapHP (Ni-NTA ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins in 10 mM acetate buffer (pH 4.5, GE Healthcare) was then injected over the chip surface at a flow rate of 30 μl/min to couple the amino groups of the recombinant proteins to the carboxymethyl dextran matrix ...
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bioRxiv - Molecular Biology 2021Quote: All recombinant proteins were purified firstly by Ni-NTA (GE healthcare). TOFU-6/PICS-1 was further purified by HiLoad 16/600 Superdex 75 column (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a GSTrap column and elution with 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a HiTrap TALON column and eluted with 50 mM sodium phosphate ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Recombinant protein was first purified on HisTrap HP column (GE Healthcare) using Buffer A (50mM Tris Base ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant proteins were purified by glutathione Sepharose 4B (GE Healthcare) or Amylose Resin High Flow (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... recombinant proteins were purified using Ni-Sepharose affinity chromatography (GE Healthcare). Final elution was carried out at 250 mM imidazole ...