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Citations for GE Life Sciences :
101 - 150 of 2216 citations for 7 Hydroxy 4 methoxy 1 indanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... overnight at 4°C followed by a 1/10.000 dilution of anti-mouse IgG HRP-linked whole secondary antibody (GE Healthcare NA931), during 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... buffer-exchanged with DPBS buffer (pH 7) and purified over Superdex 200 Increase 10/300 GL column (GE Healthcare) in DPBS buffer ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Isoelectric focusing (IEF) was performed using 7-cm Immobiline DryStrips (pH 3–11 nonlinear) (GE Healthcare Life Sciences, USA) in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained in 1x DPBS containing 0.5% bovine serum albumin fraction (BSA, pH 7, GE Healthcare, Pasching, GER) and 0.05% sodium azide NaN3 (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... and mCherry (5% laser power, 0,05 exposure) were recorded at 7 minutes intervals and data were analyzed using SoftWoRx (GE Healthcare). The time from nuclear envelope breakdown (NEBD ...
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bioRxiv - Biophysics 2021Quote: ... The grids were blotted for 7 s at force 10 using single-layer Whatman filter papers (1442-005, GE Healthcare) before plunging into liquid ethane using an FEI MarkIV Vitrobot at 8 °C and 100% humidity ...
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bioRxiv - Immunology 2021Quote: ... supernatant was adjusted to pH=7 and loaded onto a High-Trap Prot G column (GE Healthcare, Chicago, Illinois, USA) using an Äkta system (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and then immobilized in 7 cm IPG strips (pH 3-10 : 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG ...
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Genetics 2021Quote: ... Cells were then disrupted by sonication and after centrifugation (30 min at 30000 × g at 4°C) the supernatant (soluble fraction) was purified by FPLC on a 1 ml HisTrap FF column (GE Healthcare; Milan, Italy) by stepwise elution ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated at 4°C for at least 1 hr with ∼40 µL of appropriate bead slurry (nProtein A Sepharose, GE Healthcare, 5280-01). Following incubation ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2024Quote: The OVA protein was first attached to an oligonucleotide using a succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) linker and purified using a NAP column (GE Healthcare Life Sciences). Unconjugated oligonucleotides were removed by 30K Amicon filtration (Sigma) ...
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bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared at 26,200g for 30 min at 4°C and supernatants were incubated with pre-washed Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 90 min at 4°C with mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was supplemented with 4 mM CaCl2 and stirred for 2 h in 4°C with CaM-Sepharose beads (GE Healthcare), pre-equilibrated with binding buffer (50 mM Tris/HCl pH 7.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-FAM-UGUUCUUCUGGAAGUCCAGTT-3’) was separated by gel electrophoresis (20% polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... 0.125 pmol of FAM-labeled RNA product were separated by gel electrophoresis (20 % polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were resolved on 10-14 % polyacrylamide gels containing 7 M urea and analyzed with a Typhoon TRIO Phosphorimager (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...
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bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
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bioRxiv - Cell Biology 2021Quote: ... 3D widefield datasets were acquired using a 100x 1.4NA Uplan S Apochromat objective on a DeltaVision 2 Ultra microscope equipped with 7-Color SSI module and sCMOS camera and controlled by Acquire Ultra acquisition software (GE Healthcare), computationally deconvolved using the enhanced ratio constrained iterative deconvolution algorithm ...
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bioRxiv - Microbiology 2020Quote: ... Germany) and differentiated into macrophages by culture for 7 days in RPMI1640 medium supplemented with 10% fetal bovine serum (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Low-molecular-weight RNAs were separated on 6% acrylamide/7 M urea gels and transferred to a Hybond-XL membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Microbiology 2022Quote: ... and analysed by denaturing 7% polyacrylamide gel electrophoresis and by phosphorimaging with a Typhoon FLA-9500 instrument and ImageQuant TL software (GE Healthcare). Potential Cu2+ scavenging-by-buffer effects (Mash et al. ...
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bioRxiv - Neuroscience 2022Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
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bioRxiv - Plant Biology 2024Quote: ... gel containing 7 M urea and buffered with 0.5 X TBE using an SE600 standard dual-cooled gel electrophoresis unit (GE Healthcare, USA) until the bromophenol blue dye reached the bottom of the gel ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl pH 7) and size exclusion chromatography was performed using HiLoad 16/600 Superdex 75 pg Column (GE28-9893-33, GE Healthcare) attached to BioLogic DuoFlow Chromatography system (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... and 5 μg of total RNA was separated by 15% (wt/vol) acrylamide/7 M urea gel and subsequently transferred to a Hybond-NX nylon membrane (GE healthcare) after gel electrophoresis ...
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bioRxiv - Biochemistry 2023Quote: ... Purification of His-tagged domains was achieved by nickel-affinity using purification buffer supplemented with 1 mM DTT and gel filtration chromatography in 50mM ammonium bicarbonate pH 7 using a Superdex 200 Increase 10/300 column (GE Healthcare) following published protocols67 ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was desalted by a Zeba spin desalting column (7 kDa MWCO) and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare). Fluorescein- labeling efficiency was analyzed by SDS-PAGE and fluorescence gel imaging (Amersham Typhoon FLA 9500 ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was desalted by a Zeba spin desalting column (7 kDa MWCO) and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare). Fluorescein-labeling efficiency was analyzed by SDS-PAGE and fluorescence gel imaging (Amersham Typhoon FLA 9500 ...
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bioRxiv - Molecular Biology 2024Quote: ... 140∼150 uL of 6∼7 mg/mL of each complex solution was injected into the superdex200 increase 10/300 (GE Healthcare) column ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...