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Citations for GE Life Sciences :
101 - 150 of 5300 citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Glutathione-sepharose 4 beads (GE Healthcare) were added to remove the cleaved tags and non-cleaved protein for another 2 h at 4°C in a column under gravity flow ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were rinsed twice and washed 4 x 5 minutes in 1X TBST and developed using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare RPN2232), according to the manufacturer’s suggestions ...
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bioRxiv - Biochemistry 2022Quote: ... The combined eluants were incubated overnight at 4 °C with 5 mL of Glutathione-Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were placed in a column and washed 3 times with 50 mL of Buffer B (480 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... The fusion protein was cleaved with 10% (w/w) TEV protease overnight at 4°C and the mixture was loaded on a 5 ml HisTrap™FF column (GE Healthcare). 6xHis-tag-labeled disulfide bond isomerase and TEV protease were retained ...
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bioRxiv - Microbiology 2024Quote: ... The crude cell extract was cleared by centrifugation for 30 min at 16,000 ×rpm (4 °C) and loaded onto a 5 ml HisTrap™ HP affinity column (GE Healthcare, USA) equilibrated with buffer B ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was cleaved for 16 h at 4°C using 1U of thrombin (GE Healthcare) per 100 μg of total protein ...
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
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bioRxiv - Synthetic Biology 2024Quote: The OVA protein was first attached to an oligonucleotide using a succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) linker and purified using a NAP column (GE Healthcare Life Sciences). Unconjugated oligonucleotides were removed by 30K Amicon filtration (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... GFP or mCherry fluorescence was measured 4-5 days post-infiltration (see Table S3 for raw data) using the ImageQuant LAS 4000 luminescent imager (GE Healthcare Life Sciences) with the appropriate excitation and emission settings (EX ...
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bioRxiv - Biophysics 2020Quote: ... DCS experiments with working buffer solutions containing 4 μM of an SBD-mutant and 5 mM substrate were conducted on a VP-DSC Calorimeter (Micro Cal™, GE Healthcare, Buckinghamshire). The melting temperature Tm was determined by ORIGIN 8 (Origin Lab Corp ...
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bioRxiv - Biochemistry 2021Quote: ... followed by hydrophobic interaction chromatography (HIC) using a 5 mL HiTrap Phenyl FF (HS) column (GE Healthcare). For the ion exchange procedure ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Immunology 2020Quote: Columns of 1 ml Protein G sepharose 4 fast flow (GE Healthcare) were prepared and equilibrated with 5 volumes of binding buffer (0.02 M sodium phosphate ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM DTT and 4 ml of Q Sepharose resin (GE Healthcare) was mixed to capture GLH-1 protein molecules ...
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bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
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bioRxiv - Immunology 2020Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification ...
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Plant Biology 2024Quote: ... at a 1 to 20 ratio in Buffer A overnight at 4°C followed by a subtractive His trap purification using a 5 mL HisTrap HP column (GE Healthcare, Little Chalfont, UK) (GE Healthcare ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_R: 5’-TAGAGGTACCctcgagCTACTCAATGCCGAACGTGTTG-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI WWI_F: 5’-ccgGAATTCCCACCATTGCCGCCTG-3’ CG42797XhoI WWII_R: 5’-ccgCTCGAGTCAACGAGGATCCATGAA-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_Nterm_R: 5’-CCGctcgagTCATTCGCTGGGCTGC-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare).
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bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... pH 4 to 7 (GE Healthcare, United Kingdom), and left for passive rehydration for 16 h ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
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bioRxiv - Biochemistry 2021Quote: ... purified OxlT was mixed with purified 20D033-Fv at a 1:2 molar ratio at 4 °C overnight and purified using Superdex200 Increase 10/300 GL (GE healthcare) in 20 mM MES-KOH ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared at 26,200g for 30 min at 4°C and supernatants were incubated with pre-washed Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 90 min at 4°C with mixing ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: ... and on one 5 ml StrepTrap HP column (GE Healthcare, US) equilibrated in binding buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: Custom siRNAs targeting 5’-CACCAUGAGUGGCAGUCAG-3’ 33 for FRA1 and 5′-GUUCACUGCUGGCCUAUAA-3’ 34 for FLI1 were ordered from GE Healthcare Dharmacon ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Biophysics 2021Quote: ... Reactions were incubated overnight at 4°C and resolved over a Superose 6 Increase 10/300 GL column (GE Healthcare) preequilibrated with assembly buffer ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Biophysics 2023Quote: ... CFTR was purified by gel filtration chromatography at 4 °C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with 0.006% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... 1 ml of NHS-Activated Sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) was washed with 10-15 column-volumes of 1 mM HCl ...