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Citations for GE Life Sciences :
101 - 150 of 2500 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was cleaved with Precission™ protease for 2 hours at room temperature and applied to a 5 mL HiTrap Q HP column (GE Healthcare). Protein was eluted from the Q HP column with a 2M NaCl buffer in a 100 mL gradient ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 500 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA FPLC (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3 mL fractions ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 250 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA Purifier (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3-ml fractions ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Immunology 2024Quote: ... to 2 ml and subsequently loaded on a Sepharose 4 Fast Flow resin column (10 mm x 300 mm; GE Healthcare, Little Chalfont, UK), connected to the ÄKTA pure system (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...
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bioRxiv - Immunology 2024Quote: ... IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare, Life Sciences) dependent on culture volume ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted peak fractions were loaded at 2 mL/min in Buffer A onto a 5 mL HiTrap Heparin column (GE Healthcare Life Sciences) and eluted at 2 mL/min with a 50 mL gradient from 30% to 100% Buffer B ...
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bioRxiv - Microbiology 2023Quote: ... 17µl (∼ 5 µg) of each Cy-5-labelled (GE Healthcare) cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... 5 % glycerol into two 5 mL MBP-trap cartridges (GE Healthcare) connected in tandem before removal of the Ni-NTA cartridges ...
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bioRxiv - Cell Biology 2020Quote: ... The mixture was incubated for 2 hours at RT and antibodies were isolated using illustra™ NAP-5 columns (GE Healthcare Life Sciences™), resulting in an average labeling rate between 0.8 and 1.
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated in Buffer B ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was then filtered through a 0.2 µm syringe filter before being loaded onto 2 x 5 mL StrepTrap HP Columns (GE Healthcare Life Sciences 28-9075-47), equilibrated in Step A Buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
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bioRxiv - Biochemistry 2023Quote: ... Glutathione-sepharose 4 beads (GE Healthcare) were added to remove the cleaved tags and non-cleaved protein for another 2 h at 4°C in a column under gravity flow ...
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bioRxiv - Microbiology 2024Quote: ... 2 µMnt ssDNA (Ovio 45) and 2 mM ATP (GE Healthcare) in a 40 µL reaction solution containing 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... diluted 1:10,000 in 2% TBST with 2% Blocking (GE Healthcare), was applied for 1 h.
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bioRxiv - Immunology 2024Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0-0.5 M NaCl in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0–0.5 M NaCl in 20 mM Tris (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... Cy®5 (GE Healthcare) anti-mouse secondary antibody was used ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% FBS (Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30 min at 37°C and 120 rpm ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% FBS (Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30-60 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluate was further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were pooled and protein purity was assessed by SDS–PAGE and Coomassie staining ...
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bioRxiv - Biophysics 2021Quote: ... followed by ion exchange purification using a mono-Q HR 5/5 column (GE Healthcare). Purified Nap1 was snap frozen in buffer containing 10 mM HEPES pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... packed in water in a 5 mm inner diameter Tricon 5/100 Column (GE Healthcare, USA). Packing occurred by combined sedimentation and a steady flow of 100 µl/min through the column ...
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bioRxiv - Microbiology 2020Quote: ... GSTrap columns (5 mL; GE Healthcare) were used with an Akta Prime FPLC system (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... 5 ml of HisTrap (GE Healthcare) was used for the affinity purification and Gel filtration was performed on HiLoad 16/60 Superdex 200 (GE Healthcare) ...
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bioRxiv - Physiology 2022Quote: ... including 5% serum (Hyclone, GE Healthcare), 1% penicillin-streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted and further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 and 1 mM CaCl2 and applied to a 5 mL HisTrap Excel (GE Healthcare). Afterwards the column was washed with 40 mL of 20 mM TRIS pH 8 ...
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bioRxiv - Biophysics 2021Quote: ... the H3 and H4 monomers were purified using a mono-Q HR 5/5 column (GE healthcare) and SP ion exchange resin (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... All the nucleosomal DNA fragments were purified using ion-exchange chromatography (Source Q 5/5, GE Healthcare) and isopropanol precipitation ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluate was further purified by chromatography on a Mono Q HR 5/5 column (GE Healthcare) with a linear gradient of 0.1-1.0 M sodium chloride in Nap1 chromatography buffer (20 mM Tris-Cl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... see SI Table 5): Clarified lysate was applied to a 5 mL HisTrap FF column (GE Healthcare) equilibrated with His-binding buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... The reduced 5′ thiol-modified ssDNA was purified into water using NAP-5 columns (GE Life Sciences). The reduced 5’ thiol-modified ssDNA was spin vacuum dried ...
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bioRxiv - Neuroscience 2020Quote: ... pGEX-4T-2 (GE Healthcare), pGIPZ control shRNA ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
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bioRxiv - Plant Biology 2020Quote: ... pGEX6p-2 (GE healthcare, IL) and pBE2113-GFP (Yoshida et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare), 0.5% penicillin-streptomycin (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... pH 4 to 7 (GE Healthcare, United Kingdom), and left for passive rehydration for 16 h ...