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Citations for GE Life Sciences :
101 - 150 of 3428 citations for 2 Bromo 1 5 bromofuran 2 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice, GE Healthcare, 1:10000, Chicago, Illinois, USA). The bands were detected using chemiluminescence methods (ECLPrime® ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
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bioRxiv - Cell Biology 2020Quote: LC3B (amino acids 1-120) was expressed in BL21 pLysS DE3 E.coli cells from a pGEX-6P-2 plasmid (GE Healthcare) in 250 ml LB medium ...
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bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Images were taken at 2 second intervals for 1 minute and deconvoluted with Applied Precision softWorx imaging software (GE Healthcare). The microtubule comet tracking and quantification was performed with the MTrackJ plugin in ImageJ/Fiji (Meijering et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined with 2-D Quant kit (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... and captured using a HiTrap IMAC FF Ni+2 (GE Healthcare) affinity column followed by size exclusion chromatography purification with a Superdex 200 10/300 increased column (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM PMSF) on a Q sepharose fast flow (GE Healthcare) and incubated over-night ...
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bioRxiv - Microbiology 2020Quote: ... ∼2 μL mixture was blotted onto nitrocellulose membrane (Amersham; GE Healthcare) and allowed for diffusion and drying ...
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bioRxiv - Microbiology 2020Quote: ... ~2 μL mixture was blotted onto nitrocellulose membrane (Amersham; GE Healthcare) and allowed for diffusion and drying ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We used the 2-D Quant Kit (GE Healthcare, Uppsala, Sweden) to determine protein concentrations.
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bioRxiv - Microbiology 2020Quote: ... coli Rosetta 2 from pGEX-6P1 expression vector (GE Healthcare, USA). To improve the solubility of the full-length protein ...
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bioRxiv - Synthetic Biology 2020Quote: ... Blots were blocked with 2% ECL Prime blocking agent (GE Healthcare) in PBS-T (PBS buffer supplemented with 0.1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... 2 ml of EAH Sepharose 4B resins (GE Healthcare Life Sciences) were washed with distilled water three times and mixed with 10 mg of DS (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Contaminants were removed with 2-D Clean-up kit (GE Healthcare), and in-tube trypsin digestion was performed ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg carboxylate modified magnetic beads (GE Healthcare Sera-Mag™) at 1:1 (hydrophilic/hydrophobic ...
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bioRxiv - Molecular Biology 2023Quote: ... 2) SEC on Superdex 200 Hiload26/600 (GE Healthcare Life Sciences) in 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM β-mercaptoethanol) using gel-filtration NAP column (GE Healthcare) and concentrated by ultrafiltration to 1.2 ...
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bioRxiv - Physiology 2024Quote: ... utilizing a tandem arrangement of 2 Superose 6 columns (GE Healthcare). The elution process entailed the collection of fractions in 0.5 ml increments ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were loaded on 2 ml HisTrap beads columns (GE Healthcare) functionalized with Nickel and equilibrated with Buffer A ...
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for 1 hour prior to purification on a Superdex 200 10/300 size-exclusion column (GE Healthcare). Rac1 (G12V ...
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bioRxiv - Biophysics 2021Quote: ... After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare) on an FPLC (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: 50 μl of the N protein samples (1 mg/ml) purified according to protocols 1 or 2 were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Peak fractions were diluted with 2 volumes of buffer H and loaded onto a 1-mL SP HP column (GE Healthcare) and washed with buffer C (50 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Immunology 2022Quote: ... mononuclear cells were isolated by density gradient centrifugation of PBS-diluted buffy coat/blood (1:2) over Ficoll-Paque Plus (GE Healthcare). The PBMC layer was carefully removed and washed 3 times with PBS.
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bioRxiv - Cancer Biology 2022Quote: ... Blood was diluted 1:2 with 1× phosphate-buffered saline (PBS) and transferred to a 50 ml falcon tube with Ficoll-Paque PLUS (GE Healthcare) at a 1:2 ratio ...
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bioRxiv - Biochemistry 2021Quote: ... purified OxlT was mixed with purified 20D033-Fv at a 1:2 molar ratio at 4 °C overnight and purified using Superdex200 Increase 10/300 GL (GE healthcare) in 20 mM MES-KOH ...
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bioRxiv - Cell Biology 2023Quote: ... were used at a dilution of 1:10,000 and the HRP-linked sheep F(ab’)2 fragment to mouse IgG (GE Healthcare, NA9310) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Biophysics 2022Quote: ... The TOP3A-RMI1/2 complex was assembled by incubating purified TOP3A and RMI1/2 (1:3 ratio) followed by purification using a Superdex 200 Increase (GE Healthcare) in buffer I.
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bioRxiv - Biochemistry 2022Quote: ... and passed through two subsequent gel filtration steps using a: 1) Hiload 16/60 Superdex 75 column (Cytiva #28989333) and 2) and Sephacryl S-300 column (GE Healthcare). Buffers used for this sizing step and subsequent labeling reactions were followed as for full-length proteins ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a dilution of 1:10,000 and the HRP-linked donkey F(ab’)2 fragment to rabbit IgG (GE Healthcare, NA9340) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... both extracts (Triton and SDS) were diluted 2:1 in 50 nM Tris-HCl and incubated with 50 µl Streptavidin Sepharose (GE Healthcare) overnight at 4°C while rotating ...
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bioRxiv - Molecular Biology 2024Quote: An appropriate amount (0.5 – 2 µg) of total RNA was separated in 1% MOPS-formaldehyde agarose gels and transferred to Hybond-N + membranes (GE Healthcare). Membranes were exposed to either randomly [32P]-labelled dsDNA probes ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM dithiothreitol) and loaded onto Superdex S200 column (GE Healthcare) The column was equilibrated and the protein was eluted with buffer C (20 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... and subcloned into the pGEX 4T-2 vector (GE Healthcare Life Sciences) for expression in BL21 bacteria ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.2 using a 2×5ml HiTrap desalting column (GE Life Sciences) and concentrated to 10 mg/mL in a centrifugal concentrator ...
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bioRxiv - Biophysics 2022Quote: ... the supernatants were incubated with 2 mL glutathione-Sepharose beads (GE Healthcare) at 4°C for 3 hrs ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT) was passed through HisTrapTM FF (GE Healthcare Life Sciences) column ...
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bioRxiv - Biochemistry 2021Quote: ... and applied to tandem fast-flow Ni+2-NTA HisTrap (GE Healthcare) columns ...
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bioRxiv - Immunology 2022Quote: ... 2 mM CaCl2 using a HiPrep 26/10 Desalting column (GE Healthcare) and subsequently treated with enterokinase overnight at room temperature to remove the strep-tag ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice ...
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bioRxiv - Immunology 2023Quote: ... C4BPα1-2 was applied to a HiTrap Q HP column (GE Healthcare) and eluted using a 0-1 M NaCl gradient in 50 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Images for Figure 2 were obtained using a DeltaVision Elite (Applied Precision) maintained at 37°C and 5% CO2 equipped with a 40x and 63x PLANApo S lens (Olympus ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with 2 mL Ni-NTA resin (GE Healthcare), equilibrated with buffer A (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... RMI1/2 was further purified using a Superdex 200 Increase (GE Healthcare) in buffer I ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP) and applied to a 25 ml Source15Q (GE Healthcare) strong anion exchange column equilibrated in buffer A ...