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Citations for GE Life Sciences :
1401 - 1450 of 1450 citations for Anti CD117 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Recombinant RBD protein at a concentration 20 μg/ml in 10 mM acetate buffer (pH 4.5) was immobilized on the surface of a CM5 sensor chip using a Amine Coupling Kit (GE Healthcare Bio-Sciences AB, Sweden). VHHs were serial 4-fold diluted from 417 nM to 0 nM and flowed over the captured RBD surface at 15 μl/min for 3 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The samples were transferred to a positively charged membrane using southern blot protocol from the Whatman Nytran SuPerCharge TurboBlotter kit (Whatman GE Healthcare, Little Chalfont, UK). Air dry the membrane ...
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bioRxiv - Microbiology 2022Quote: ... Multiple displacement amplification of genomic DNA was performed using the illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (GE Healthcare, Piscataway, NJ, USA). Paired-end library was constructed using NEXTFLEX Rapid DNA-Seq (Bioo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The remainder of the extraction was performed with a modified version of the Illustra RNAspin mini RNA isolation kit (GE Healthcare, #25-0500-71) protocol ...
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bioRxiv - Cell Biology 2024Quote: ... PCR samples were resolved on agarose gel and amplified fragments extracted from gel using GFX PCR DNA and Gel Band purification kit (GE Healthcare, 28-9034-70). The purified DNA or 1µg of plasmid DNA were digested with XmaI and XbaI for 1 h at 37°C in 1x CutSmart buffer and nuclease-free water ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibody solution was dumped and the membrane washed three times for 10 minutes each with 1xTBST before adding the polyclonal Anti-Rabbit IgG Peroxidase (GE Healthcare; NA9340V; 16908235) at a 1:5000 dilution in milk ...
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bioRxiv - Cancer Biology 2019Quote: ... Chemiluminescence detection was performed after incubation with the secondary antibody ECL (Anti-Mouse IgG Horsradish Peroxidase linked whole antibody: GE Healthcare UK limited) for 2h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... For detection of RNA polymerase (RNAP), anti-RNAP (Neoclone, #WP003) antibody and antimouse IgG antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, Code#: NA931). Immunoblots for ClpP or RNAP served as loading control ...
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bioRxiv - Developmental Biology 2021Quote: ... The bound protein was detected using rabbit anti-GH antibody at a final dilution of 1:40,000 (AFP5672099, NIDDK-NHPP) followed by horseradish peroxidase-conjugated antibody (anti-rabbit, IgG; GE Healthcare, Chicago, Illinois, USA) at a final dilution of 1:2000 ...
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bioRxiv - Molecular Biology 2019Quote: ... characterization of an N-terminal Aβ peptide DAE-EG (DAEFRHDSGYSGKQKSRNEGKGGC) binding to anti-mouse captured antibodies from hybridoma supernatants was performed using a BIAcore T-200 instrument (GE Healthcare, Marlborough, MA) [36] ...
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bioRxiv - Cell Biology 2019Quote: Binding affinities were measured using purified and immobilized GST-SH3Boi1 or GST-SH3Boi2 as ligands on an anti-GST chip on a Biacore X100 device (GE Healthcare, Freiburg, Germany). HBSEP buffer was used as running buffer in all experiments ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed to remove unbound phages and then probed with anti-M13-HRP secondary antibody (1:5000, Cytiva [formerly GE Healthcare], Marlboro, MA) followed by TMB as noted above.
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated in PBST containing a 1:2500 diluted secondary antibody (horseradish peroxidase-linked anti-mouse IgG from sheep; GE Healthcare, Cat. NA931V). After 3 rounds of 5-min wash with PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... Analysis was done by western blotting using the primary antibodies directed against H3K36me3 (ab9050, 1:2000) or H3K36me2 (ab9049, 1:2500) and as secondary antibody the anti-rabbit HRP (Na934v, GE Healthcare, 1:5000). The signal was detected by chemiluminescence after the addition of Pierce™ ECL Western Blotting substrate.
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed and incubated for 30 min with horseradish peroxidase-conjugated goat anti-rabbit IgG (GE Healthcare Bio-Sciences, NJ, USA). Labeling was performed using an enhanced chemiluminescence (ECL ...
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bioRxiv - Biochemistry 2023Quote: ... H2AX (#2595S; Cell Signaling technology]. The membranes were extensively washed with 1% TBST followed by incubation with anti-isotype secondary antibody (GE Healthcare) conjugated with horseradish peroxidase in 5% skimmed milk at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: Single cell suspension was collected by centrifugation (3 min at 300 g) and total RNA was extracted by Illustra™ RNAspin RNA Isolation Kit (GE Healthcare GE25-0500-72) according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 μL of the purified DNA was amplified by Multiple Displacement Amplification (MDA) with the Genomephi V3 kit (GE Healthcare Life Science, Marlborough, MA, USA) for 30 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Probes were labled with 32P-dCTP (Hartmann Analytic, Braunschweig) by the use of the Rediprime II DNA Labeling Kit (GE Healthcare Life Science, Little Chalfont) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were then extracted and purified using an illustra™ GFX™ PCR DNA and Gel Band Purification kit (GE Healthcare, Buckinghamshire, UK). The samples were sequenced using the general Sanger sequencing technique.
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bioRxiv - Plant Biology 2022Quote: ... chromosomal DNA was purified and amplified to 5.4 - 7.9 μg by multiple displacement amplification (Supplementary Table 3) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by Šimková et al ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with developing solution kit (Amersham™ ECL Select™ Western Blotting Detection Reagent) and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ ...
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bioRxiv - Cell Biology 2020Quote: ... Peroxidase-linked anti-mouse immunoglobulin G and peroxidase-linked antirabbit immunoglobulin G (both Cytiva, formerly known as GE Healthcare Bio-Sciences NA931 and NA934), Alexa Fluor 594-conjugated Donkey Anti-Mouse IgG (1:250 ...
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bioRxiv - Developmental Biology 2020Quote: ... The membranes were washed with Tris buffered saline containing Tween 20 (AppliChem; TBST) and incubated with secondary anti-rabbit (GE Healthcare; Chicago, IL, US) or anti-mouse (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was then incubated with primary antibody diluted in TBST/BSA for one hour followed by a one hour incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:10,000, GE Healthcare life sciences, Piscataway, NJ). Primary antibodies used were ...
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bioRxiv - Immunology 2020Quote: Approximately 200 μL of normal mouse serum or anti-CD200 serum day 157 (after 4X immunizations) were purified using a protein G SpinTrap (GE Healthcare # 28-4083-47) according to the manufacturer’s instrutions ...
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bioRxiv - Neuroscience 2021Quote: ... IRDye® 680LT goat anti-rabbit IgG (H + L) (LiCor, cat. #926-6802, 1:10,000) and horseradish peroxidase (HRP)-conjugated goat anti-chicken IgG (GE Healthcare, cat. #AS09603, 1:10,000).
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bioRxiv - Plant Biology 2021Quote: ... or anti-S8H (Tsai et al., 2018) as primary antibodies followed by detection with secondary antibodies (Agrisera; AS09 602, and GE Healthcare; A934-100UL, respectively). The chemiluminescence signal detection was performed using Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... and anti–α-catenin shRNA (α-cat-KD) (both courtesy of G.D. Longmore, Washington University) were cultured in DMEM/F12 (GE Healthcare Life Sciences), supplemented with 5 % horse serum (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... Amplified phage particles at 10-fold dilution were assayed against 20 nM biotinylated membrane proteins in nanodiscs using horse radish peroxidase (HRP)-conjugated anti-M13 monoclonal antibody (GE Healthcare, #27-9421-01). Assays were performed in library sorting buffer (200 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBST for 5 min and incubated for 1 h at room temperature with HRP-conjugated secondary anti-rabbit IgG (GE Healthcare, NA934, 1/2000) or anti-mouse IgG (Amersham ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 1:1000 diluted Amersham ECL anti-rat IgG horseradish peroxidase-linked species-specific whole antibody (NA935, GE Healthcare UK Limited, UK) or Amersham ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody (NA931 ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were washed (3x) with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, dilution 1:10.000) or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Physiology 2023Quote: ... with TBS-T and incubated (1-hour, room temperature) with HRP-linked anti-rabbit secondary antibody (1:10,000; GE Healthcare Bioscience, Baie d’Urfe, Canada). Chemiluminescence was assessed using the Luminata™ Crescendo Western HRP Substrate (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed with TBS containing 0.1% Tween 20 (3 times for 15 min under shaking at RT) and probed with an anti-rabbit HRP conjugated antibody (NA934V, GE healthcare, Piscataway, NJ, USA) for 2 h at RT ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and successful triplicate reactions were pooled and cleaned using the Illustra ExoProStar PCR and Sequence Reaction Clean-Up Kit (GE Healthcare Life Sciences, Pittsburgh PA, USA). Indexing adaptors were added via PCR (8 cycles ...
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bioRxiv - Plant Biology 2023Quote: ... after which their DNA was amplified by multiple displacement amplification (MDA) (Table S1) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by (13) ...
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bioRxiv - Microbiology 2024Quote: The same mixture of phage DNA as mentioned above was amplified by Multiple Displacement Amplification (MDA) using the Genomephi V3 kit (GE Healthcare Life Science, Marlborough, MA, USA) with 10 ng input and incubated at 30°C for 0.5 h (MDA_0.5h ...
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bioRxiv - Biophysics 2019Quote: ... The membrane was rinsed three more times in TBS-Tw and incubated for 30 min with secondary antibody (HRP-conjugated sheep anti-mouse IgG, GE Healthcare Life Sciences; Freiburg, Germany) diluted 1:20,000 in TBS-Tw + 1% milk ...
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bioRxiv - Physiology 2021Quote: ... Results were normalized to the expression of β-actin (cat#: A3854, source: mouse, 1:80,000, Sigma-Aldrich; anti-mouse IgG (GE Healthcare; dilution: 1:25,000). Immunoreactive bands were visualized with an enhanced chemiluminescence detection system (ChemiDoc Imaging System ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution in 3% milk overnight at 4°C before incubation with secondary HRP-linked sheep anti mouse (NA931-1ML, GE Healthcare Life Sciences, Buckinghamshire, UK) at a 1:3000 dilution in 5% milk for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare, Chicago, IL, United States). The signal was visualized in a Chemidoc MP imaging system (Bio Rad)s).
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bioRxiv - Genomics 2019Quote: ... cell lysis and whole-genome amplification steps were carried out in Fluidigm C1 system using illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences cat: 25-6600-30). DNA concentrations of amplified single-cell genomes were determined using either a Qubit dsDNA HS Assay kit (Invitrogen cat:Q32854 ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, cat. #NA932, dilution 1:10,000), or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were washed three times with TBST for 10 minutes each and exposed to donkey anti-rabbit secondary antibody conjugated to horse radish peroxidase (GE Healthcare; NA934V; LOT: 16976257; 1:10,000) in TBST containing 5% (w/v ...
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bioRxiv - Developmental Biology 2019Quote: ... PVDF membranes were subsequently incubated for 1 hour at room temperature with donkey HRP-linked anti-rabbit secondary antibody (1:5000 for FATP1, FATP4, FAT/CD36; 1:10000 for FABPpm, GE Healthcare, Bio-Science, Baie d’Urfe, QC, Canada) or donkey anti-goat secondary antibody (1:5000 for β-actin ...