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Citations for GE Life Sciences :
1401 - 1450 of 4447 citations for 8 Chloro 2 3 4 5 tetrahydropyrido 3 2 f 1 4 oxazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... A 20-μl droplet of virus inoculum containing 8X106 plaque-forming unit (PFU) of PRV-Becker, resuspended in medium (Dulbecco modified Eagle medium, 2% fetal calf serum and antibiotics) (Hyclone, GE Healthcare life sciences), was applied onto the abraded area of the skin ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Cell Biology 2023Quote: ... was used according to manufacturer’s guideline to clone cDNAs into pUASTattB (kindly provided by K. Basler, University of Zurich) and pGEX-6P-2 (GE Healthcare Life Sciences). Full length (FL ...
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bioRxiv - Microbiology 2024Quote: ... MUC5B was purified using a Bio-Rad NGC fast protein liquid chromatography (FPLC) system equipped with an XK 50 column packed with 2 L of Sepharose CL-2B resin (GE Healthcare Bio-Sciences). Mucin-containing fractions were identified using a periodic acid-Schiff’s reagent assay and analysis of UV absorbance at 280 nm from FPLC ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 8% heat-inactivated fetal bovine serum (HyClone, GE Healthcare Life Sciences), and maintained at 37°C under 5% CO2.
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bioRxiv - Neuroscience 2020Quote: ... positions 8 and 85 for γSyn) were captured on GS4B beads (GE Healthcare). GST-synucleins were reduced with 1 mM DTT for 20 min at 4 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... pH 8) and loaded on a Superdex 75 10/300 GL (GE Healthcare) size exclusion column to remove s10 ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 8% heat-inactivated fetal bovine serum (HyClone, GE Healthcare Life Sciences), 0.075% sodium bicarbonate (Gibco Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... 20 mM Tris-HCl pH 8 using PD-10 desalting columns (GE Healthcare). 10 μM CAD was hydroxylated by incubation with 10 μM FIH ...
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bioRxiv - Bioengineering 2023Quote: ... Nitrocellulose membranes (8 μm) were from Cytiva (formerly GE Healthcare Life Sciences, UK) and silicon metal powder (3 μm ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP, 5 mL; GE Healthcare) that had been previously equilibrated with Ni-NTA Buffer (NB ...
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bioRxiv - Biochemistry 2019Quote: ... FLAG eluate was loaded onto a 1 mL HisTrap™ High-Performance 5 mL columns (GE Healthcare) using a peristaltic bump and washed with 5 column volumes of 26S NiA buffer (30 mM HEPES pH 7.6 ...
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bioRxiv - Cell Biology 2019Quote: Time lapse imaging of cultured primary neurons (in Schneider’s/FCS) and fibroblasts (in Ham’s F-12/FCS) was performed on a Delta Vision Core (Applied Precision) restoration microscope using a [100x/1.40 UPlan SAPO (Oil)] objective and the Sedat Quad filter set (Chroma #89000) ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBST for 5 min and incubated for 1 h at room temperature with HRP-conjugated secondary anti-rabbit IgG (GE Healthcare, NA934, 1/2000) or anti-mouse IgG (Amersham ...
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bioRxiv - Bioengineering 2019Quote: ... 2 mL of human buffy coat/fresh blood mixed well with 2mL of phosphate-buffed saline (PBS) and then carefully layered on top of 2 mL of Ficoll (GE Healthcare Life Sciences, US) inside a 10ml centrifuge tube without mixing ...
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bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
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bioRxiv - Molecular Biology 2020Quote: ... Protein were separated on 4-15 % Tris-glycine (TG) gels (#45101080003-2, SMOBIO, Hsinchu, Taiwan) and electro-transferred onto polyvinylidene fluoride (PVDF) membranes (#A16646282, GE Healthcare, Chicago, IL, USA) in TG buffer ...
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bioRxiv - Immunology 2021Quote: ... The blots were washed in TBS-T and incubated in secondary antibody in antibody buffer for 2 hours before washing again in TBS-T before visualising with ECL (GE Healthcare, Chicago, IL, USA) and imaging in an ALLIANCE 6.7 Chemiluminescence Imaging System (UVITEC ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following the manufacturer’s protocol using 15 µl of PCR product and 2 µl Streptavidin Sepharose High Performance beads (GE Healthcare, cat. GE17-5113-01).
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bioRxiv - Molecular Biology 2023Quote: ... 300 μM 5’ thiol-modified 18-nt DNA oligonucleotides—comprised of an AAA base sequence and a 15-base secondary probe binding sequence (Eurofins)—were reduced with 50 mM dithiothreitol in PBS at room temperature for 2 hours and purified using NAP5 columns (GE Healthcare 17-0853-01). Maleimide-activated antibodies were then combined with 11 equivalents of the reduced form of the thiol-modified DNA oligonucleotides in PBS and incubated at 4℃ overnight ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biochemistry 2022Quote: The HMWP2-Cy2 supernatant was loaded at 1 ml/ min onto a 5 mL HisTrap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Filtered lysate was loaded onto a pre-equilibrated 1 mL or 5 mL HisTrap HP column (GE Healthcare) charged with Ni2+ ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) and then loaded on a HiTrap Heparin column (CV, column volume 5 ml; GE Healthcare), equilibrated with buffer A ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Neuroscience 2022Quote: ... equipped with an 8-channel receive-only head array coil (GE Healthcare, Milwaukee, WI). First ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions of 8 ml were collected in an automated AKTA FPLC system (GE Healthcare) and purity was determined with SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... Enrichments were performed in test tubes containing 5 mL of M63 minimal media and a 1 x 10 cm strip of Whatman #1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA) pressed against the side of the tube (30) ...
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bioRxiv - Microbiology 2021Quote: ... equilibrated in PBS pH 7.4 and NiV-F was purified using a Superose 6 Increase 10/300 GL column (GE healthcare) equilibrated in 10 mM Tris and 150 mM NaCl pH 8.0.
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bioRxiv - Genomics 2023Quote: ... and lysed by resuspension in 20 ml cold F Buffer (18% w/v Ficoll-PM400 (GE Healthcare 17-0300-50), 40 mM potassium phosphate ...
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bioRxiv - Immunology 2019Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0-0.5 M NaCl in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0-0.5 M NaCl in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0–0.5 M NaCl in 20 mM Tris (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... Cy®5 (GE Healthcare) anti-mouse secondary antibody was used ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was collected and incubated for 5 min on 1 mL Ni Sepharose® 6Fast Flow (GE Healthcare) equilibrated with 10 mL binding buffer ...
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bioRxiv - Biophysics 2021Quote: ... 10 μM ZnCl2 and 1 mM DTT) and loaded into a HiTrap Heparin HP 5 ml column (GE Healthcare) equilibrated with 6.25% of buffer HepB (buffer HepA supplemented with 2 M NaCl) ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was loaded at 1 ml/min on a His-Trap FF-crude 5 mL column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mM DTT and loaded directly onto a 5 ml HiTrap SP Sepharose fast flow column (GE Healthcare). Bound Nup84-Nup133 was eluted from the column using a 15 column volume (CV ...
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Biochemistry 2019Quote: ... The water phase was loaded to a MonoQ 5/50 GL (1 ml) column (GE17-5166-01, GE Healthcare). ppGpp was eluted with a linear gradient from 0.5 mM to 600 mM LiCl in buffer C (25 mM Tris-HCl pH 8.3 ...
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bioRxiv - Neuroscience 2023Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (1-5 mg/ml) were loaded individually on a Superdex 200 Increase 10/300 column (GE Healthcare), and the elution profiles were obtained using a tandem of sequentially connected UV-Vis Prostar 335 (Varian ...
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bioRxiv - Cell Biology 2020Quote: ... were activated by 100 mM DTT for 2 hours at room temperature in the dark and then purified using NAP5 columns (GE Healthcare Life Sciences, 17-0853-02) to remove excessive DTT ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...