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Citations for GE Life Sciences :
1351 - 1400 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Phosphorylated proteins were recovered by gel filtration on Superdex 200 Increase (GE Healthcare) in 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were initially purified using Ni Sepharose 6 Fast Flow resin (GE Healthcare). The protein tags were cleaved with His-tagged TEV-protease overnight at 4 °C while dialyzing against TEV cleavage buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Purified recombinant proteins were loaded onto Superdex 75 10/300 column (GE Healthcare) in 150mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Buffer exchanged protein was applied to a 5mL HiTrap QHP column (GE Healthcare,) using a 30% gradient of QB buffer (20mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated and loaded onto a Superose 6 column (GE Healthcare) pre-equilibrated in purification buffer and peak fractions were pooled and concentrated to 5-6 mg/mL.
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bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were visualized by chemiluminescent detection (ImageQuant LAS 4000, GE Healthcare Life Sciences) of peroxidase substrate activity (SuperSignal West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on SDS– polyacrylamide gel were transferred onto a nitrocellulose membrane (GE Healthcare). The bands were visualized utilizing Enhanced Chemiluminescence (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... and purified by fast protein liquid chromatography using Calmodulin-Sepharose (GE Healthcare, USA). TAT-CaM was expressed in BL21(DE3)pLysS E ...
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bioRxiv - Cell Biology 2021Quote: ... GST-fusion proteins were purified by incubation with glutathione-Sepharose beads(GE Healthcare). The precipitates were washed 3X with lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... Excess dye was removed by purifying protein over NAP-5 columns (GE Healthcare). Labeling was confirmed using NanoDrop 2000c (Thermo Fisher).
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified by Ni2+-IMAC on HisTrap columns (GE Healthcare). Fractions ...
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bioRxiv - Microbiology 2020Quote: ... Spike protein was finally purified using Superose 6 HR10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES (pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... the protein solution was applied to a HiTrapTM Q HP column (GE Healthcare) and eluted with linear gradient of 0.15-2.0 M of NaCl in 10 mM Tris (pH 8.0) ...
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bioRxiv - Immunology 2020Quote: ... The recombinant GST fusion proteins were affinity purified on GSH-Sepharose (GE Healthcare) from bacterial cultures incubated with 0.25 mM isopropyl-β-D-thiogalactopyranoside (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... mRNA was purified by Fast Protein Liquid Chromatography (FPLC) (Akta Purifier, GE Healthcare), as described 74 ...
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bioRxiv - Immunology 2021Quote: ... by incubating sera with a resin of protein G Sepharose beads (GE Healthcare) for 2h ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by gel filtration chromatography (Superdex-200, GE Healthcare) with columns equilibrated in storage buffer (50 mM Tris-HCl 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to nitrocellulose membranes (GE Healthcare Life Sciences – REF 10600003), which were incubated with the appropriate antibodies according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant proteins were purified with a fast flow Ni-NTA column (GE Healthcare) and eluted by 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biophysics 2020Quote: ... the reduced protein was bound to a Ni2+-Sepharose resin (GE Healthcare, UK) and washed with 10 column volumes of 50 mM KPi (pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were wet transferred to nitrocellulose membrane (0.45 μm, GE Healthcare Life Sciences) at 100 V for 75 min ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were visualized using the ImageQuant LAS500 chemidocumenter (GE Healthcare, Chicago, USA). The ECL signal was visualized in the chemiluminescent channel ...
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bioRxiv - Cancer Biology 2022Quote: ... Buffer replacement in protein purification used Column PD 10 desalting column (GE Healthcare). All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were purified on a Ni-NTA HiTrap HP column (GE Life Sciences) and the His tag was removed by overnight cleavage with TEV protease (homemade) ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was desalted using a PD10 column (GE Healthcare, Cat. no. 17085101) in 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... NQO1 proteins were purified using immobilized nickel affinity chromatography columns (IMAC, GE Healthcare) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... Tagged proteins were purified by affinity chromatography (HisTrap resp. StrepTrap, both GE Healthcare) with elution buffer containing 200 mM imidazole respectively 2.5 mM d-desthiobiotin ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified by SEC on a Superdex 75 (GE Healthcare) column ...
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bioRxiv - Microbiology 2022Quote: ... The dimeric ACE2 protein was purified by GammaBind Plus Sepharose beads (GE Healthcare), followed gel filtration chromatography on a Superdex 200 Increase 10/300 GL column ...
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bioRxiv - Microbiology 2022Quote: ... Materials and equipment for protein purification were obtained from GE Healthcare (Munich, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... GST fusion proteins were immuno-precipitated using Glutathione Sepharose 4B beads (GE Healthcare). Fusion protein-bound beads were used directly in GST pull-down assays ...
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bioRxiv - Microbiology 2022Quote: ... protein was loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) with TBS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins separated on gels were electroblotted onto nitrocellulose (Protran 0.2 μm, GE Healthcare) with a constant current of 100 mA for 10 h using wet transfer ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were precleared by incubating with protein G-Sepharose beads (GE Healthcare), and reacted with 100 μL of Strep-Tactin Sepharose beads (IBA Lifesciences ...
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bioRxiv - Molecular Biology 2022Quote: ... on an ÄKTA explorer fast protein liquid chromatography (FPLC) system (GE Life Sciences) in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... were applied along with Protein G Sepharose 4 Fast Flow beads (GE Healthcare). All ChIP extracts were pre-cleaned for 1 hr with 30 μl beads on a rotor at 4°C in absence of the antibody ...
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bioRxiv - Immunology 2022Quote: ... The separated proteins were transferred on Amersham Protran nitrocellulose membrane (GE Healthcare Lifescience). After blocking with 5 % skimmed milk in TBS-T (0.1 % Tween-20 (Sigma-Aldrich ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...
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bioRxiv - Neuroscience 2024Quote: ... and subsequently incubated with Protein A Sepharose beads (#17-0780-01, GE Healthcare). After washing with a NOD2 protein purification buffer containing 0.1% Triton X-100 ...
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Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... Immunoreactive proteins were detected with ECL Prime Western Blotting Detection Reagent (GE Healthcare) using an ImageQuant LAS 500 device (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatants were combined with Protein G Sepharose beads (GE Healthcare, Illinois, USA) and incubated at 4 °C for an hour ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences).
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bioRxiv - Neuroscience 2024Quote: ... recombinant IgLON5 or LG1 protein was spotted onto a nitrocellulose membrane (GE Healthcare), membranes were blocked with 1% BSA in PBS ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-Fc was purified using a Protein-A affinity column (GE Healthcare). NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... GST fusion proteins were purified on Glutathione-Sepharose® 4B beads (GE Healthcare) and eluted using a solution of 50LmM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2024Quote: ... Lysate pre-cleaning steps were performed using Protein G Sepharose beads (GE Healthcare). To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... supernatants were harvested and IgG was purified on Protein G Sepharose (GE Healthcare)
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified using size exclusion chromatography (Superdex S75; GE Healthcare).