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Citations for GE Life Sciences :
1351 - 1400 of 1534 citations for Anti CD130 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... except that total extracts were subjected to pull-down using monoclonal antibodies against Cherry bound to protein G-Sepharose 4 Fast Flow beads (GE Healthcare). The suspensions were end-over-end mixed on a rotating wheel for 2 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3x for 10 min with TBST and subsequently incubated for 1 hr at room temperature with appropriate horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) diluted (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualized by incubation with the indicated antibodies and developed using the chemiluminescence function on an Amersham Imager 600 (GE Healthcare) or a Compact 2 developer (Protec) ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were incubated with indicated primary and HRP-conjugated secondary antibodies then detected using Amersham ECL Western blotting reagents (GE Healthcare) or for more sensitive detection SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... was used as the secondary antibody. Protein was detected using the chemiluminescent substrate ECL (Cat. # 32209, Pierce) in an Amersham Imager 680 (GE Healthcare) detector.
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bioRxiv - Developmental Biology 2023Quote: ... Then it was incubated with a secondary antibody and detected using a Medical Film Processor (SRX-101A) or ImageQuant LAS 4000 (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were washed three times in PBST for 15 min and were then incubated with HRP-conjugated secondary antibodies (1:2,500 dilution; GE Healthcare, NA9340V) for 45 min at room temperature ...
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bioRxiv - Immunology 2023Quote: The binding kinetics and affinity of monoclonal antibodies to the SARS-CoV-2 spike trimer were analyzed by SPR (Biacore T100, GE Healthcare). Specifically ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were subsequently washed with TBST (0.1% Tween-20) and incubated with an HRP-conjugated secondary antibody (GE Healthcare Bio-Sciences). After washing with TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were detected with host-specific secondary antibodies and developed with a chemiluminescent substrate (GE Healthcare Amersham, Piscataway, NJ, USA). After stripping ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was loaded into gravity column containing anti-FLAG M2 affinity gel (GE Healthcare). The resin was washed with wash buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2020Quote: ... The bound HY5 was detected by anti-GST-HRP conjugate (RPN1236; GE Healthcare Bio-Sciences). Membranes were developed and visualized as described above ...
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bioRxiv - Systems Biology 2020Quote: ... washed in TBST and detected with Sheep anti-Mouse IgG-Horseradish peroxidase (HRP; GE Healthcare Life Science ...
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bioRxiv - Biochemistry 2020Quote: ... and HRP-linked anti-mouse (GE Healthcare, #NA931-1ML, at a dilution of 1:5000) as the secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... mAb followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2,000, NXA931, GE Healthcare). Periplasmic fractions ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories, 315-035-003 or GE Healthcare, #NA931).
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bioRxiv - Immunology 2022Quote: ... followed by staining with the horseradish peroxidase (HRP)-conjugated anti-rabbit (1:50,000; GE healthcare) and anti-mouse (1:25,000 ...
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bioRxiv - Cell Biology 2019Quote: ... Blots were incubated with horseradish peroxidase (HRP)-coupled sheep anti-mouse IgG (GE Healthcare, NA931VS) and (HRP)-coupled goat anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... or ECL Horseradish Peroxidase-linked anti-mouse (NA931) (GE Healthcare Life Sciences, Little Chalfont, UK) secondary antibody diluted 1:20000 ...
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bioRxiv - Microbiology 2020Quote: ... followed by goat anti-rabbit IgG–HRP and detection with ECL detection Reagent (GE Healthcare) or Coomassie blue staining were used as loading controls.
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bioRxiv - Biochemistry 2020Quote: ... Horseradish peroxidase-conjugated anti-rabbit IgG (1:10,000, GE Healthcare Life Sciences, Fairfield, CT, USA) was used as the secondary antibody ...
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bioRxiv - Microbiology 2021Quote: ... or Amersham ECL peroxidase-linked Sheep anti-Mouse IgG (GE Healthcare Bio-Sciences, Uppsala, Sweden) as secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated for 1 hour with anti-rabbit (NA934, GE Healthcare; RRID: AB_772206) antibody diluted in TBS-T containing 5% milk ...
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bioRxiv - Biophysics 2023Quote: ... Horseradish-peroxidase (HRP)-conjugated secondary Abs: sheep anti-mouse IgG (1:2000, GE Healthcare, #NXA931), goat anti-Rat IgG (1:2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Then they were incubated with horseradish peroxidase (HRP) conjugated Sheep anti- Mouse IgG (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... they were incubated with an anti-rabbit HRP-conjugated (1:2500, Amersham NA934, GE Healthcare) secondary antibody in 1% bovine serum albumin in Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 and anti-rat IgG HRP secondary at 1:2500 (GE Healthcare, NA935).
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bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
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bioRxiv - Immunology 2022Quote: IgG was purified from cell culture media or plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Cell Biology 2021Quote: ... Postnuclear supernatants were incubated with 3 μg of antibodies coupled to protein A– or protein G–Sepharose beads (GE Healthcare, Solingen, Germany) for 4 h at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... The conjugated antibody was purified by SEC (Superdex 200 increase 10/300 GL, GE Healthcare, solvent: PBS, flow rate = 0.6 mL/min) to afford an antibody–linker conjugate [1.91 mg ...
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bioRxiv - Bioengineering 2021Quote: ... Slides were then washed twice with TBST (TBS with 0.05 % wt/v Tween 20) before incubating with the appropriate biotinylated secondary antibodies (GE Healthcare, 1:500) for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
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bioRxiv - Immunology 2019Quote: Total IgG antibodies were captured from 2 µL of plasma using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Developmental Biology 2019Quote: ... Antibodies used for immunoblotting are described in Table S3 HRP-conjugated anti-rabbit secondary antibody was used (Table S3) and protein detection was performed with ECL Select Western Blotting Detection Reagent (GE Healthcare, RPN2235) on an ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were detected using horseradish peroxidase-conjugated secondary antibodies and detected using ECL (RPN2106) or ECL-select (RPN2235) reagents (Amersham, GE Healthcare, UK) and a cooled digital camera (LC33000 ...
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bioRxiv - Immunology 2023Quote: FH was isolated from human plasma using an affinity column made up of a monoclonal antibody against human FH (MRCOX23; MRC Immunochemistry Unit, University of Oxford) coupled to CNBr-activated Sepharose (GE Healthcare, UK) as described previously (21) ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution; CtNo_NA931, GE Healthcare, Tokyo, Japan), developed with Immobilon Western Chemiluminescent HRP Substrate (CtNo_WBKLS0100 ...
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bioRxiv - Microbiology 2023Quote: ... Anti-rabbit immunoglobulin AP-conjugated secondary antibody was used and reaction results were obtained using CDP Start Detection Reagent kits (GE Healthcare, USA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was washed with 0.1% TBST and incubated with corresponding secondary antibody conjugated to Horse Radish Peroxidase (HRP) at 1:10000 (GE life sciences; NA934) dilutions for one hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then washed in TBST and incubated for 1 hour at room temperature with HRP-conjugated secondary antibody (1:10000 in PBS; GE Healthcare/Biocat) Membranes were washed three times in TBST before bands were visualised using ECL (GE Healthcare) ...