Labshake search
Citations for GE Life Sciences :
1301 - 1350 of 1366 citations for Rabbit Anti Human IgG gamma chain Alexa Fluor 750 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... were used in combination with secondary anti-mouse antibodies conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). For visualization ...
-
bioRxiv - Immunology 2019Quote: ... anti-mouse immunoglobulin (Ig)-G horseradish peroxidase (HRP)-linked secondary antibody (Amersham catalogue number NA931, GE Healthcare, Buckinghamshire UK) diluted 1:4000 was incubated at RT for 2 hours before final washes and 3,3’,5,5’-Tetramethylbenzidine (TMB ...
-
bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used were horseradish peroxidase (HRP)-conjugated anti-mouse antibody (sheep polyclonal, used at 1:3000, GE Healthcare), HRP-conjugated anti-rabbit antibody (sheep polyclonal ...
-
bioRxiv - Biochemistry 2021Quote: GST-MLLE protein was applied to Series S sensor chip CM5 bound with anti-GST antibodies (GE Healthcare BR100223), in Biacore T100 ...
-
bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
-
bioRxiv - Immunology 2021Quote: Kinetics binding measurement for anti-spike mAbs in this study was performed using a Biacore T200 instrument (GE Healthcare). The system was flushed with filtered 1xHBS-P+ running buffer (0.01M HEPES ...
-
bioRxiv - Microbiology 2022Quote: ... For all primary mouse antibodies an anti-mouse-HRP in a dilution of 1:2000 was used (GE Healthcare). All blots were blocked with TBS-T 5% Milk and washed 3x for 15 minutes with TBS-T after each antibody incubation ...
-
A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... a CM5 sensor chip was covalently modified by an anti-GST polyclonal goat antibody (GST capture Kit, GE Healthcare). The surface of flow cell 1 and 2 was activated for 60 s with 50 mM NaOH (10 μL/min) ...
-
bioRxiv - Cancer Biology 2022Quote: ... using HRP-conjugated secondary anti-mouse antibody (2.5:10 000) and Amersham ECL prime detection reagent (GE healthcare lifescience).
-
bioRxiv - Cancer Biology 2023Quote: ... A875 cell extracts were incubated for 16 hr at 4°C on a rotating wheel with 0.8μg of anti-p75NTR antibody (ANT-007, Alomone) and then incubated with Sepharose protein-G beads (GE Healthcare). The beads were collected by brief centrifugation (2 min ...
-
bioRxiv - Genetics 2023Quote: ... Blots were washed 3 times with 1x TBS-T and incubated with anti-mouse HRP (GE Healthcare Cat # NXA931) or anti-rabbit HRP secondary antibody (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2024Quote: ... was used in combination with a secondary anti-mouse antibody conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). Primary rabbit anti-mCherry antibody (BioVision 5993 1:2000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... The following antibodies have been conjugated to fluorochromes using commercially available kits according to the manufacturer’s instructions: anti-S100B has been conjugated to FITC (FLUKA) using Illustra NAP-5 columns (GE Healthcare), anti-GD2 (ch14:18 ...
-
bioRxiv - Neuroscience 2019Quote: ... and HRP-labelled anti-mouse secondary antibody before bands being detected using an enhanced chemiluminescence kit (GE Healthcare, Buckingham, UK). β-actin (Sigma ...
-
bioRxiv - Cell Biology 2019Quote: ... The detection of tubulin using mouse anti-tubulin KMX-1 primary (1:100; Gift from Keith Gull, Oxford) and anti-mouse-HRP secondary (1:20,000, GE Healthcare) was used to verify equal sample loading ...
-
bioRxiv - Immunology 2019Quote: ... were captured via their histidine tags onto CM5 chips pre-coupled with ~9000 reflective units anti-His Ab (GE Healthcare) using standard amine chemistry ...
-
bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Western blot of the immunoprecipitated recombinant proteins was performed as described in the above section using anti-GST HRP (horseradish peroxidase) conjugate (1:10,000 Amersham GE Healthcare) for 60 minutes at room temperature under agitation ...
-
bioRxiv - Biochemistry 2021Quote: ... Polyclonal anti-GST antibody (Carl Roth, 3998.1) was covalently immobilized to all four flow cells of a CM5 sensorchip (GE Healthcare) to a level of 8,000 response units (RU ...
-
bioRxiv - Cell Biology 2019Quote: ... we coupled 130 μg of a mouse monoclonal anti-H4K12Ac (gift from Hiroshi Kimura) to 12.5 μl rProtein A Sepharose (GE Healthcare) slurry in antibody coupling buffer (10 mM K-HEPES pH=8 ...
-
bioRxiv - Biochemistry 2024Quote: ... The washing step was followed by 30 min incubation with HRP-conjugated Mouse Anti-M13 monoclonal antibody (GE Healthcare, 27942101) diluted in PBST in 1:5000 ratio ...
-
bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was mixed with pre-equilibrated anti-GFP nanobody (GFPnb)-coupled cyanogen bromide-activated Sepharose beads (GE Healthcare) at 4 °C for 2 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... Anti-Strep blots were imaged using an Amersham Imager 600 and Amersham ECL Prime Western Blotting Detection Reagent (GE Life Sciences).
-
bioRxiv - Molecular Biology 2021Quote: ... RNAi efficiency testing for ELAV was done from dissected central brains by Western blotting according to standard protocols as described 73 using a polyclonal rat anti-ELAV antibody generated against Drosophila ELAV (1:800) 45 and secondary HRP-coupled goat anti-rat antibody (1:5000, GE Healthcare) by chemoluminescence detection (Pierce ...
-
bioRxiv - Biophysics 2019Quote: ... The remaining lysate was then incubated overnight at 4°C with anti-GFP (Rudner et al., 1999) or anti-SMC (Lindow et al., 2002) antibodies and subsequently incubated by Protein A-Sepharose resin (GE HealthCare) for 1 hour at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were purified at 4°C on the anti-FLAG resin at a flow rate of 20 ml/h with a P1 pump (GE Healthcare), and eluted by competition with a FLAG peptide solution at 200 μg/ml in 10 mM Hepes ...
-
bioRxiv - Biophysics 2021Quote: ... Total protein was collected and incubated with anti-p75NTR (Alomone, ANT-007, 1ug) overnight at 4 °C and then incubated with Sepharose protein-G beads (GE Healthcare). Samples were then prepared for immunoblotting as described below.
-
bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h with 5 μL of anti-MYC antibody (Gentex) followed by 1 h incubation with an additional 50 μL of GammaBind plus Sepharose beads (GE Healthcare) to capture protein complexes ...
-
bioRxiv - Microbiology 2020Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare). The signal was visualized in a Chemidoc MP imaging system (BIO-RAD).
-
bioRxiv - Microbiology 2022Quote: ... Aldolase was detected using the above protocol with 1:10,000 mouse anti-aldolase antiserum (a kind gift from David Sullivan) and 1:2,500 sheep anti-mouse HRP-linked secondary antibodies (GE healthcare, NA931).
-
bioRxiv - Molecular Biology 2022Quote: Purified NiV F was immobilized to a single flow cell of either (i) a CM5 sensor functionalized with an anti-StreptagII antibody or a NiNTA sensor in a Biacore X100 (GE Healthcare). Binding kinetics for Fabs 4H3 and 1H8 were measured using a CM5 sensor ...
-
bioRxiv - Cell Biology 2019Quote: ... G2 and G1 phases based on the signal intensity of anti-CENPF antibody staining with software (IN Cell Investigator, GE Healthcare).
-
bioRxiv - Neuroscience 2019Quote: ... The blots were exposed to a peroxidase-conjugated anti-mouse secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare, Piscataway, NJ). The resulting chemilluminescent signal was acquired with a Molecular Imager Chemidoc EQ system (Bio-Rad ...
-
bioRxiv - Microbiology 2021Quote: ... between 400 to 600 RU of biotinylated anti-IFX monoclonal antibodies were immobilised on a streptavidin-coated sensor chip prepared using the Biotin CAPture kit (GE Healthcare); a biotinylated mouse monoclonal antibody (OX68 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Simultaneously 3 μg of the anti-FLAG antibody (described above) was subjected to incubation with protein-G sepharose beads (60 ul, 17061801, GE Healthcare) for 1 hour with tumbling ...
-
bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
-
bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
-
bioRxiv - Microbiology 2021Quote: ... were used for immunoblotting along with sheep anti-mouse-HRP NXA931V secondary antibody (GE Healthcare, 1:5,000 or 1:10,000, respectively). All yeast growth and Western blot data shown are representative of at least two biological replicates.
-
bioRxiv - Microbiology 2019Quote: ... clarified lysates were mixed with 1 µg anti-HA monoclonal antibody and rotated with 30 µl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 3 hours at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... to prevent activation of the alternative complement pathway and complement consumption) and anti-FH mAb (35H9) on Hi-Trap HP-NHS columns (GE Healthcare). FH protein was purified from EDTA-plasma using an anti-FH mAb (35H9 ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed and incubated with DAPI and anti-bleach reagents prior to imaging on a DeltaVision Elite Microscope (GE Healthcare). For all experiments ...
-
bioRxiv - Biochemistry 2023Quote: ... Bound proteins were eluted by boiling in SDS–PAGE sample loading buffer and analyzed by western blot using an anti-GST antibody (GE Healthcare).
-
bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
-
bioRxiv - Biochemistry 2023Quote: ... Affinity purification was carried out using a high affinity anti-GFP nanobody covalently coupled to agarose beads (NHS-activated beads, GE Healthcare) in a 3:1 ratio (mg:ml) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were then incubated with anti-BrdU (BU-1; 1:100; GE Healthcare, RPN202) and phospho-histone H2AX (Ser139 ...
-
bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies coupled to horseradish peroxidase (HRP) were applied for 1 h: sheep anti-mouse (#NA9310V, GE Healthcare, 1:4000), donkey anti-rabbit (#NA9340V ...
-
bioRxiv - Immunology 2022Quote: ... anti-PD-L1 and LH02 were all purified by affinity chromatography using a protein A affinity column (GE Healthcare, Piscataway, NJ, USA) and analyzed in reducing condition on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
-
bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
-
bioRxiv - Synthetic Biology 2022Quote: ... they were soaked in TBS-t for 30 min at room temperature with HRP-conjugated anti-mouse (GE Healthcare, Chicago, IL, USA) or anti-rabbit antibody (GE Healthcare ...