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Citations for GE Life Sciences :
1301 - 1350 of 3998 citations for Borrelia burgdorferi sensu stricto B31 P35 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The protein was passed over a size exclusion column (Superdex 200, GE Life Sciences) in buffer GF (buffer A plus 300 mM NaCl and 1 mM dithiothreitol (DTT)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant proteins were purified by affinity chromatography using glutathione (GSH) agarose beads (GE Healthcare) and dialyzed in mild lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were further purified by SEC using a Superdex 200 column (GE Healthcare, USA), in running buffer (10 mM Tris-HCl ...
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bioRxiv - Physiology 2022Quote: ... Separated proteins were transferred onto 0.2 µm Nitrocellulose membranes (catalog no 10600015, GE Healthcare) in Tris-Glycine (25 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was purified on a Ni Sepharose 6 Fast Flow column (GE Healthcare) under gravity and eluted with the elution buffer (300 mM Imidazole ...
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bioRxiv - Microbiology 2022Quote: ... the purified protein was loaded into a HiPrep 26/10 desalting column (GE Healthcare), and the buffer was exchanged to Tris 20 mM ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing target protein were desalted from imidazole using PD10 desalting column (GE Healthcare) and incubated with 50 μM ONO-5430608 and a His-tagged PreScission protease (homemade ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Protein A Sepharose™ 4 Fast Flow sepharose beads were purchased from GE Healthcare Life Sciences (Pittsburgh ...
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bioRxiv - Microbiology 2022Quote: GST-tagged protein was purified using Glutathione-based affinity chromatography (GSTrap FF GE Healthcare). Cells were thawed on ice and resuspended in Buffer F (20 mM HEPES pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Trimeric spike protein was further purified by Superose 6 size-exclusion chromatography (GE Healthcare) in 10 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed proteins were loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare) and eluted with a linear gradient of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2022Quote: ... the proteins were loaded onto a HiLoad Superdex-S200 size-exclusion column (GE Healthcare) using AKTA purifier system (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... The GST-tagged proteins were absorbed with Glutathione-Sepharose 4B affinity matrix (GE Healthcare) for 1.5hrs and recovered by 3C protease cleavage at 4°C overnight in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... purified proteins were passed through HiLoadTM 16/60 SuperdexTM 200pg (GE Healthcare Life Sciences). The purified protein was then stored in -80°C in buffer containing 25 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate containing recombinant proteins was passed through a PD-10 column (GE healthcare) to exchange buffer with Store Buffer (50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 ml column (GE Healthcare), equilibrated with buffer A (50□mM NaH2PO4 (pH 8) ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoreactive proteins were visualized using the ECL Select Western Blotting Detection System (GE Healthcare). Signals were detected on a Las-4000 mini instrument (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... 50 μl of pre-washed protein-G sepharose-4 fast flow (GE Life Sciences) was added to each tube and tumbled end-over-end for 4 h at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The protein was further purified on a Superose 6 gel filtration column (GE Healthcare) using an AKTA Pure FPLC instrument (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... The protein was first purified with a Ni2+-nitrilotriacetic acid (NTA) resin (GE Healthcare,) using an elution buffer consisting of 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... proteins were blotted onto a nitrocellulose membrane (Hybond-C Extra, GE Healthcare, Munich, Germany) Ponceau S staining of the blotted membrane was performed to assure equal amounts of proteins ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transferred onto PVDF membranes (Amersham Hybond-LFP membranes [GE Healthcare, Buckinghamshire, UK]), or nitrocellulose membranes (Amersham Protran ...
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bioRxiv - Neuroscience 2020Quote: ... The eluted proteins were further purified via gel filtration (Superdex200, GE Healthcare Chicago, IL).
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bioRxiv - Biophysics 2020Quote: ... The labelled protein was further analysed by size-exclusion chromatography (Superdex 200, GE Healthcare) to enrich the double-labelled fraction and remove potential aggregation material131 ...
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bioRxiv - Microbiology 2021Quote: ... Protein bands were developed using the ECL™ Western Blotting Detection Reagent (GE Healthcare) and chemiluminescence was visualized using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... The protein was finally purified by Superdex 75 10/300 GL column (GE Healthcare) with buffer B.
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bioRxiv - Microbiology 2020Quote: ... The full-length ectodomain proteins were purified using HisTrap FF column (GE Life Sciences) in elution buffer containing 20 mM sodium phosphate ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive protein was visualized using an enhanced chemiluminescence analysis kit (GE HealthCare, Piscataway, NJ).
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bioRxiv - Biochemistry 2021Quote: ... The eluted proteins were further purified by gel filtration (Superdex 200 column; GE Healthcare) in PBS with 5 mM β-mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant protein was desalted with PD-10 column (SephadexTM G-25M, GE Healthcare), purified using a cation exchange column [HiTrap carboxymethyl (CM ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were buffer-exchanged with PBS pH 7.4 using PD-10 columns (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The protein was then loaded onto a 5 mL HiTrap Q column (GE Healthcare) equilibrated with 50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was run through the HiLoad 16/600 Superdex 200 column (GE Healthcare) in buffer A and dialyzed against 20 mM Tris-HCl (pH 8.0 at 25 °C) ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing the eluted protein were loaded onto a HiPrep desalting column (GE Healthcare) equilibrated with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Intensities of the resultant protein bands were quantified using ImageQuant TL software (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... Immunoreactive proteins were detected with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) using an ImageQuant LAS 500 device (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were then wet-transferred to a 0.2 μm Nitrocellulose membrane (Amersham GE Healthcare) at 150 V for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... and proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 nitrocellulose, GE Healthcare) in transfer buffer (50 mM Tris-OH ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Cancer Biology 2022Quote: ... The BCA assay kit was used to quantify the isolated protein samples (GE Healthcare). The proteins were then heat-denatured and resolved on SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... IgG was isolated using 5 ml HiTrap Protein G High Performance column (GE Healthcare), whereas IgM was isolated using POROS™ CaptureSelect™ IgM Affinity Matrix (ThermoScientific ...
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bioRxiv - Immunology 2022Quote: ... and the antibodies were purified by protein A affinity chromatography (GE Healthcare Life Sciences) as previously described31 ...
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bioRxiv - Biophysics 2019Quote: ... The labelled proteins were further purified by size-exclusion chromatography (Superdex 200, GE Healthcare) using buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... Phosphorylated proteins were visualized using an FLA7000IP Typhoon Storage Phosphorimager (GE Healthcare Life Sciences) and quantified in ImageJ ...
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged proteins were purified with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01), washed and either eluted with glutathione of cleaved with PreScission protease (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Polyclonal antibody 2134 supernatent was purified using a Protein A affinity column (GE Healthcare). Serum was neutralized with 1/10 volume 1 M Tris pH 8 ...
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bioRxiv - Biophysics 2019Quote: ... The diluted protein was applied to a heparin column (HiTrap Heparin HP, GE Healthcare), washed with 20mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was further purified by cation exchange with Source S column (GE Healthcare), eluted with a linear KCl gradient of 0.1 – 1 M KCl ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were then incubated with protein G sepharose beads (GE Healthcare, 17-0618-01) for 2–3 h at 4°C and beads were washed 3 times with diluted lysis buffer (dilution of 1:3 in dH2O ...