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Citations for GE Life Sciences :
1301 - 1350 of 2143 citations for 8 3 CARBOXYPROPYL 1 3 DIMETHYLXANTHINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Calorimetric titration of Mint3NT with FIH-1 was performed using a MicroCal™ iTC200 system (GE Healthcare) at 25°C ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal His6-tag was then removed by digestion with thrombin (≈ 1 U/mg; GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Japan) was subcloned into the BamHI and XhoI sites of pGEX-4T-1 vector (GE Healthcare, Inc.) using standard PCR-based protocols ...
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bioRxiv - Biochemistry 2020Quote: ... to < 1 mL and injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EGTA (pH 8.0)) and further purified on a Source 15Q anion exchange column (GE Healthcare) with the application of 50 mL buffer I (20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysates were incubated with glutathione sepharose (1 ml bed volume; GE Healthcare #17-0756-01), washed and eluted by the addition of 2 x 1.5 ml of 10 mM reduced L-glutathione (Sigma #T6066 ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP, 5 mL; GE Healthcare) that had been previously equilibrated with Ni-NTA Buffer (NB ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µM all samples) separately was loaded (300 µl) in a superdex-200 increase column (GE Healthcare) attached to a Malvern Panalytical Omnisec resolve-reveal system ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated for 1 min with HRP substrate (ECL Prime Western Blotting Detection Reagent (GE Healthcare) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo-Fisher-Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... the membranes were probed with 1:2,500 goat anti-rat HRP-linked secondary antibodies (GE healthcare, NA935) in 5% milk powder/PBS solution for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with 1 mL IgG beads (IgG Sepharose 6 Fast Flow, GE Healthcare, 17096902) for POT1 or 1 mL amylose beads (New England BioLabs ...
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bioRxiv - Physiology 2020Quote: ... All membranes were incubated with secondary horseradish peroxidase conjugated antibody (1:10000; GE Healthcare, Chicago, Illinois, USA) for 1-2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK). Band quantification was done using Image LabTM v ...
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bioRxiv - Cancer Biology 2022Quote: ... WT and mutant Ankrd26 N-Ank modules were generated by cloning into pGEX-6P-1 (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... generated by cloning of corresponding ORFs between BamHI and XhoI sites of pGEX 4T-1 (GE Healthcare), expressed PhoPN ...
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bioRxiv - Biochemistry 2019Quote: ... Cleared lysates were supplemented with 1 mM DTT and incubated with GSH-Sepharose 4B matrix (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Samples seeded on #1 thickness coverslips were imaged on a DeltaVision Core light microscopy system (GE Healthcare) using either a 60x/1.35na or 100x/1.4na objective fitted to an Olympus IX-71 microscope stand ...
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bioRxiv - Cell Biology 2019Quote: ... Siz1 (167-508) or Siz2 (130-490) was purified from MBPTrap HP 1 mL column (GE Healthcare) with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... FLAG eluate was loaded onto a 1 mL HisTrap™ High-Performance 5 mL columns (GE Healthcare) using a peristaltic bump and washed with 5 column volumes of 26S NiA buffer (30 mM HEPES pH 7.6 ...
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bioRxiv - Plant Biology 2019Quote: ... one of the 1 mL supernatant was desalted by PD-10 desalting columns (GE Healthcare, Pittsburgh, PA) according to manufacturer’s instructions and eluted with 3 mL IP buffer ...
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bioRxiv - Physiology 2019Quote: ... and subsequently with secondary anti-mouse/ -rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 μL (24-well plates) or 0.6 μl (96-well plates) Dharmafect-1 (GE Life Sciences) in serum-free Minimum Essential Medium Eagle (MEM ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblots were washed with TBS-T and incubated with HRP-conjugated secondary antibodies (GE Healthcare, 1:5,000) for one hour in room temperature before development in films ...
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bioRxiv - Microbiology 2020Quote: ... and revealed with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000; GE Healthcare, USA). Blots were developed using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... The plexin protein in the supernatant was captured using a 1 mL HisTrap FF column (GE Healthcare) and eluted by Buffer B containing 10 mM Tris (pH 8.0) ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with anti-Rabbit HRP-conjugated secondary antibody (1:5000 of NA934, GE Healthcare) for 1 hour at room temperature and subsequently washed with PBST thrice ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was loaded onto a 1 ml HiTrap Heparin HP column (GE Healthcare, 17-0406-01) and eluted with a linear gradient from buffer A (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... and secondary horseradish peroxidase-conjugated antibodies 1:10 000 in PBS 0.1% Tween-20 (Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Plant Biology 2021Quote: ... and secondary horseradish peroxidase-conjugated antibodies 1:10 000 in PBS 0.1% Tween-20 (Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated goat against mouse IgG and rabbit IgG (1:1000 for WB) were from GE Healthcare. Rabbit pAb against SYVN1 (# 13473-1-AP ...
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bioRxiv - Cell Biology 2020Quote: ... and secondary antibodies HRP-coupled anti-mouse or anti-rabbit (GE Healthcare and Jackson Laboratories, 1:5000). The cell cycle analysis was performed using HMLE cells that were fixed in 70% ethanol for 1 h on ice ...
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bioRxiv - Microbiology 2020Quote: ... while the secondary antibody (1/10,000) was a commercial Amersham ECL-HRP linked rabbit IgG (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... to < 1 mL and injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Genetics 2021Quote: ... incubated for 1 h at 65°C and dot-blotted on Amersham HybondTM-XL membrane (GE Healthcare). DNA was fixed by UV using a UV crosslinker BLX-254 (Analytik Jena) ...
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bioRxiv - Microbiology 2020Quote: ... Eggs were collected on a wet filter-paper (Grade 1 filter paper, Whatman plc, GE healthcare, UK). Eggs were desiccated for 5 days and later hatched in deionized water containing 1g/L bovine liver powder (MP Biomedicals ...
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bioRxiv - Microbiology 2020Quote: The heterologous proteins containing His-tags were purified via 1 ml Ni-NTA HisTrap columns (GE Healthcare). The cells were lysed in 50 ml lysis buffer containing 50 mM Na-phosphate buffer pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... 0.03 % Triton-X 100) and proteins were eluted directly into a 1 mL HisTrap column (GE Healthcare) using purification buffer supplemented with Desthiobiotin 2.5 mM (IBA Solutions For Life Science™) ...
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bioRxiv - Molecular Biology 2021Quote: ... and then detected with sheep anti-mouse IgG-HRP (GE Healthcare, Little Chalfont, UK; NA931V, 1:5,000), and donkey anti-rabbit IgG-HRP (GE Healthcare ...
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bioRxiv - Genetics 2020Quote: ... These primary antibodies were detected with HRP-conjugated anti-mouse or anti-rabbit (1:5000; GE HealthCare), using the SuperSignal™ West Pico chemiluminescent substrate (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT and further purified by ionic exchange chromatography using a Mono-Q column (GE Healthcare). Protein was eluted with a linear gradient of a buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... The sample was loaded onto an immobilized metal-affinity column (1 mL, HIStrap HP column, GE Healthcare), which was washed with 50 mM sodium phosphate (NaPi ...
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bioRxiv - Microbiology 2022Quote: ... Eggs were collected on a wet filter-paper (Grade 1 filter paper, Whatman plc, GE healthcare, UK), desiccated for 5 days and later hatched in deionized water containing 1g/L bovine liver powder (MP Biomedicals ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes incubated with an anti-mouse HRP-linked secondary antibody (1:5000, GE Healthcare, NXA931, RRID:AB_772209). Either Pierce ECL Western Blotting Substrate (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... GST tagged Mcl-1 was purified using GST-affinity chromatography (Glutathione-Sepharose resin by GE Healthcare, Fastflow). Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma ...
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bioRxiv - Microbiology 2024Quote: ... were used together with horseradish peroxidase-conjugated sheep α-mouse immunoglobulin G (dilution: 1:2000) (GE Healthcare) as a secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) and separated at 0.25 ml min-1 using an AKTA Pure instrument (GE Healthcare). One ml fractions were collected ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was added to equilibrated glutathione-Sepharose 4B beads (GE Healthcare; 1 ml/100 g cells) and gently rotated for 4 h at 4°C ...